**Author details**

Yauheniya Osbon and Manish Kumar\* Department of Biology, Texas State University, San Marcos, Texas, USA

\*Address all correspondence to: m\_k135@txstate.edu

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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*Biocatalysis and Strategies for Enzyme Improvement DOI: http://dx.doi.org/10.5772/intechopen.85018*

> [8] Minteer SD. Enzyme Stabilization and Immobilization: Methods and Protocols. New York, N.Y.: Humana Press, Springer; 2017. DOI:

[9] O'Fagain C. Enzyme stabilization—

[10] Turanli-Yildiz B, Alkim CZ, Cakar P.

Engineering Methods and Applications, Protein Engineering. Rijeka: InTech; 2002. ISBN: 978-953-51-0037-9

[11] Barrozo A, Borstnar R, Marloie G, Lynn Kamerlin SC. Computational protein engineering: Bridging the gap between rational design and laboratory evolution. International Journal of Molecular Sciences [Internet]. 2012;**13**(10):12428-12460. DOI: 10.3390/

[12] Zaks A, Klibanov AM. The effect of water on enzyme action in organic media. Journal of Biological Chemistry. 1988;**263**:8017-8021. DOI: 10.1073/

[13] Klibanov A. Why are enzymes less active in organic solvents than in water? Trends in Biotechnology. 1997;**15**(3):97-101. DOI: 10.1016/

[14] Liang Y-R, Wu Q, Lin X-F. Effect of additives on the selectivity and reactivity of enzymes. Chemical Record.

2017;**17**(1):90-121. DOI: 10.1002/

[15] Lee CS, Ru MT, Haake M, Dordick JS, Reimer JA, Clark DS. Multinuclear NMR study of enzyme hydration in an organic solvent. Biotechnology and Bioengineering. 1998;**57**(6):686. DOI: 10.1002/(SICI)

10.1007/978-1-4939-6499-4

Recent experimental progress. Enzyme and Microbial Technology. 2003;**33**(2-3):137-149. DOI: 10.1016/

In: Kaumaya P, editor. Protein

S0141-0229(03)00160-1

ijms131012428

pnas.82.10.3192

tcr.201600016

S0167-7799(97)01013-5

[1] Benkovic S, Hammes-Schiffer S. A perspective on enzyme catalysis. Science. 2003;**301**(5637):1196-1202. DOI: 10.1126/science.1085515

[2] Glazer AN, Nikaido H. Microbial Biotechnology : Fundamentals of Applied Microbiology [Internet]. Cambridge; New York: Cambridge University Press; 2007. pp. 398-429. DOI: 10.1017/CBO9780511811227.012

[3] Tunon I, Moliner V. Simulating Enzyme Reactivity: Computational Methods in Enzyme Catalysis, RSC Theoretical & Computational Chemistry Series. England: Royal Society of Chemistry; 2017. DOI: 10.1039/9781782626831-FP001

[4] Abir BB, Nadine MSM, Alaa A-A. An organic solvent-stable lipase from a newly isolated *Staphylococcus aureus* ALA1 strain with potential for use as an industrial biocatalyst. Biotechnology

2016;**63**(3):378. DOI: 10.1002/bab.1381

and Applied Biochemistry.

[5] Matsuzawa T, Watanabe M, Yaoi K. Improved thermostability of a metagenomic glucose-tolerant betaglycosidase based on its X-ray crystal structure. Applied Microbiology and Biotechnology. 2017;**101**(23-24):8353-8363.

DOI: 10.1007/s00253-017-8525-9

[6] Martínez AT, Ruiz-Dueñas FJ, Camarero S, Serrano A, Linde D, Lund H, et al. Research review paper: Oxidoreductases on their way to industrial biotransformations.

Biotechnology Advances. 2017;**35**:815-831. DOI: 10.1016/j.

[7] Kaul P, Asano Y. Strategies for discovery and improvement of enzyme function: State of the art and opportunities. Microbial Biotechnology. 2012;**5**(1):18-33. DOI: 10.1111/j.1751-7915.2011.00280.x

biotechadv.2017.06.003

**References**

*Biocatalysis and Strategies for Enzyme Improvement DOI: http://dx.doi.org/10.5772/intechopen.85018*
