Meet the editors

Masashi Arita received his PhD degree in Solid State Physics from ETH Zurich, Switzerland, in 1987. He is now an associate professor at the Graduate School of Information Science and Technology, Hokkaido University, Sapporo, Japan. His major subject at present is in-situ electron microscopy of electronic devices.

Norihito Sakaguchi received his PhD degree in Materials Science from Hokkaido University, Japan, in 1999. He is now an associate professor at the Graduate School of Engineering, Hokkaido University, Sapporo, Japan. His major subject at present is nano-analysis using analytical transmission electron microscopy in metals and semiconductors.

Contents

Electron Pulses *by Jinfeng Yang*

of Langerhans Islets

*by Abeer A. Abd El Samad*

*by Ronald E. Gordon*

*by Sei Saitoh*

of Medicine

**Preface III**

**Chapter 1 1**

**Chapter 2 21**

**Chapter 3 35**

**Chapter 4 59**

**Chapter 5 69**

Ultrafast Electron Microscopy with Relativistic Femtosecond

Electron Microscopic Recording of Myosin Head Power and Recovery Strokes Using the Gas Environmental Chamber

Correlative Light-Electron Microscopy (CLEM) and 3D Volume

Imaging of Serial Block-Face Scanning Electron Microscopy (SBF-SEM)

Transmission Electron Tomography: Intracellular Insight for the Future

Analytic Analyses of Human Tissues for the Presence of Asbestos and Talc

*by Haruo Sugi, Tsuyosi Akimoto and Shigeru Chaen*

## Contents


Preface

Since the inventions of the transmission electron microscope (TEM) and the scanning electron microscope (SEM), these instruments have greatly contributed to the progress of various research fields, such as crystallography, metallurgy, materials science and engineering, biology, medical science, etc. Intensive

operation software.

contaminants are discussed.

other research fields.

research and development of instrumentation, electron optical theory, and methodology have been performed by many researchers and technicians, and imaging quality has been improved. In the last few decades, because of developments in field emission (FE) electron sources and aberration correctors, highly resolved imaging is now widely available by using up-to-date electron microscopes such as the aberration-corrected TEM/STEM and FE-SEM. In addition, analytical imaging has become possible in less time because of the development of detectors and

In this tide of microscopy, various microscopic methods have also been developed to make clear many unsolved problems. For example, high-speed investigations are possible using a pulse beam TEM and environmental microscopy enables observations in the atmosphere. Other examples include electron microscopy combined with other measurement systems like in-situ TEM and correlative microscopy, and tomography giving 3D information of engineering materials and biological tissues. In addition, development of methodologies in sample preparation is also an impor-

In this book, we have collected a number of examples concerning these subjects. In Chapter 1, a relativistic ultrafast electron microscope with femtosecond electron pulses and ultrahigh voltage microscopy is reviewed. In addition to details of instrumentation, observation results to evaluate its performance are shown. In Chapter 2, an example of environmental microscopy applied to biology is reviewed. Using a specially designed system, clear movement of a myosin head under different conditions in a gas atmosphere is demonstrated. In Chapter 3, correlative light-electron microscopy and serial block face scanning electron microscopy are reviewed. Following a general introduction of these methods and detailed experimental procedures, results of human and mouse pancreases are demonstrated. In Chapter 4, applications of high-voltage transmission electron tomography to biomedical investigations are reviewed. Various examples to investigate cell organelles are shown. In Chapter 5, TEM applications in environmental medicine are reviewed. Specified topics here are the diseases caused by asbestos and talc fibers. Procedures and protocols of the sampling of these powders contained in human tissues without

Although this book includes a limited number of topics, we think that the content in each chapter will be impressive to the reader. We hope this book will contribute to future advances in electron microscopy, materials science, biomedicine as well as

tant issue to achieve results reflecting original sample characteristics.
