**3. The spectrum of microarray applications on endometriosis**

A large number of microarray gene-specific cDNA are fixed on a glass or silica using the hybridization principles to detect the mRNA of the different sources. This study shows the different organization, different cells and tissues in different developmental stages that have differentially expressed genes. Development of molecular mechanisms provide theoretical basis for gene diagnosis and treatment of cutting-edge biotechnology. The theory proposed by Sampson in 1927 suggests that endometrial tissue is released into the peritoneal cavity via retrograde menstruation. The shed tissue then implants and grows ectopically. This theory is supported by the fact that up to 76% -90% of women experience retrograde menstruation ; and yet, endometriosis only affects 10% -15% of women. Reference to foreign literature, different individuals sample of patient with endometriosis geometric mixed, different individuals sample of patient without endometriosis geometric mixed, to eliminate non-specific genetic differences between individuals, and search for specific associated genes with endometriosis. By the gene microarray expression profiling 22,000 points compare ectopic endometrium and normal ectopic endometrial of the Uyghur and Han chinese with endometriosis, 11 differential genes expressed in ectopic endometrium were

7979 PAEP -5.57639 Progestagen-associated endometrial protein (placental

1813 TIMP3 -2.748361 Tissue inhibitor of metalloproteinase 3 (erythroid

Table 2. Han with and without endometriosis ectopic endometriosis differential genes.

The incidence of endometriosis has no precise information. Researchs have found that Asian women with endometriosis have a higher prevalence, and its disease risk :OR:8.6(95%CI ll.4一20.7). There was an exploratory study suggested that there might be an associated risk of endometriosis for those women who have worked as a flight attendant, service station attendant, or health worker, particularly a nurse. But they have not been reported the

Clinical and epidemiological survey found that endometriosis has a genetic predisposition and significant family aggregation, and it loss of heterozygosity of 40% -70%. Dingyan(researcher in Xinjiang) found that no evidence was found to suggest an association between GSTM1-null genotype and endometriosis in the Hans chinese and Uyghurs. An association was found between GSTT1 -null genotype and endometriosis in the Hans chinese, but not in the Uyghurs. The two ethnic groups have different genetic predisposing factors to the development of endometriosis. There were significant difference in the frequencies of these two points among the Han chinese, European and Uyghur in Xinjiang. In Uyghur the distribution of CYP 1 A 1 /MspI genotypes were different from Han chinese

A large number of microarray gene-specific cDNA are fixed on a glass or silica using the hybridization principles to detect the mRNA of the different sources. This study shows the different organization, different cells and tissues in different developmental stages that have differentially expressed genes. Development of molecular mechanisms provide theoretical basis for gene diagnosis and treatment of cutting-edge biotechnology. The theory proposed by Sampson in 1927 suggests that endometrial tissue is released into the peritoneal cavity via retrograde menstruation. The shed tissue then implants and grows ectopically. This theory is supported by the fact that up to 76% -90% of women experience retrograde menstruation ; and yet, endometriosis only affects 10% -15% of women. Reference to foreign literature, different individuals sample of patient with endometriosis geometric mixed, different individuals sample of patient without endometriosis geometric mixed, to eliminate non-specific genetic differences between individuals, and search for specific associated genes with endometriosis. By the gene microarray expression profiling 22,000 points compare ectopic endometrium and normal ectopic endometrial of the Uyghur and Han chinese with endometriosis, 11 differential genes expressed in ectopic endometrium were

**2. The incidence of endometriosis of Uyghur ethnic group in Xinjiang** 

correlation between nationality, religion and other factors with endometriosis.

**3. The spectrum of microarray applications on endometriosis** 

**Description**

protein 14, pregnancy-associated endometrial a

potentiating activity, collagenase inhibitor)

**ID Name Rate of** 

(Continuation)

and European.

**Cy5/Cy3**

screened out between Uyhgur women with or without endometriosis respectively, FOS, DCN, VIN, GNGS, XCL 1, IGFBP7, PRS23, TIMP3, COL3A1, PRL29, GAPD; GAPD expression in the three loci, including FOS, DCN, VIN, GNGS, XCL1, IGFBP7, PRS23 were up-regulated, and TIMP3, COL3A1, PRL29, GAPD were down-regulated. The Han chinese group were significantly different genes, 58 of which TIMP3, PAEP, GADP were downregulated, but GADP expressed in three loci shows different range. And from a different CD74, ACTA2, GPX3 and other 55 genes were upregulated, ACTB appear in 17 loci, GNGS appear in two loci. The same genes difference between the two groups is VIM, GNGS, PRS23, GAPD, TIMP3, including GAPD, TIMP3 are down-regulated. We get different genes according to their main function and are divided into the following categories: immunerelated genes,proto-oncogenes and tumor suppressor genes,cell receptor,ion channels and transport protein; cytoskeleton and sports-related protein, apoptosis-related protein; DNA synthesis and repair, recombinant protein, DNA binding, transcription and transcription factors,cell signaling and transmission white and some unknown functional genes.
