**Abstract**

Microsoft Excel was recently added to the list of software applications for signal and image processing. The use of Excel as a powerful tool for teaching signal and image data processing techniques as demonstrated in agriculture and natural resource management can be easily adopted for biomedical applications. In the same vein, Excel's proven utility as a research tool can also be harnessed. This chapter expands the methodology of signal and image formation, visualization, enhancement, and image data fusion using Excel. Different types of techniques used in biomedical imaging are introduced, including: X-ray radiography (X-rays), computerized tomography (CT), ultrasound (U/S), magnetic resonance imaging (MRI), and optical imaging. However, the chapter mainly focuses on optical imaging involving a single spectrum or multiple spectra such as RGB. Specific illustrations of corresponding outputs from different techniques are discussed in the chapter for a better appreciation by the reader.

**Keywords:** optical imaging, multispectral imagery, image visualization, image enhancement, RGB image data, RGB2X, RGBExcel
