**3.2 Selectivity**

It was determined by elimination of noise at the retention time of the compound, which is performed by fixing two transitions of MS/MS for individual molecule of analyte by considering the adequate precursor and product ions.

### **3.3 Sensitivity**

Detection limit (LOD) in the chromatogram was calculated by using peak signal of the analyte molecule concentration to the three times background noise in the chromatogram. The quantification limit (LOQ ) in the chromatogram was set as the lowest concentration with very good recovery range (65–100%) and precision (RSD ≤ 20%). The ion ratio (Q/q) was used for the criterion of confirmation in positive samples. The Q/q is the ratio of the intensity quantification (Q ) and confirmation transition (q) (**Table 1**).

Typical S/N acceptance criteria: LOD—3:1 and LOQ—10:1.
