**6. Conclusion**

Grapes contaminated with residues of pesticides pose a major health hazard. Therefore we have developed effective method for the detection of contaminated grapes. Hence, for the simultaneous confirmation and quantification of 35 pesticides in grape samples, a mulitresidue method has been developed and validated. For multi-class pesticide residue determination, GC-MS/MS with triple quadrupole analyzer played an important role. Within 22 min of run time, all the closely eluted and co-eluted peaks were separated with higher sensitivity. The two MRM transitions, one for confirmation another for quantification, achieved very good

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provided the original work is properly cited.

© 2019 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/ by/3.0), which permits unrestricted use, distribution, and reproduction in any medium,

1 P.G. Department of Chemistry, Karnataka University, Dharwad, Karnataka, India

, Mahadev B. Chetti<sup>2</sup>

2 Indian Council of Agricultural Research, New Delhi, India

\*Address all correspondence to: drbilehal@gmail.com

and Dinesh C. Bilehal1

\*

*Gas Chromatographic-Mass Spectrometric Detection of Pesticide Residues in Grapes*

sensitivity and selectivity for possible safe identification by the use of Q/q ratio parameter. The limit of detection was lower than the MRL prescribed. Solid-phase extraction with acetonitrile solvent was employed. Finally, the method was successfully validated for two concentrations, viz. 0.02 and 0.05 mg/kg grape sample. The validated method reduces the overall cost of analysis and also offers lowuncertainty measurement. Further, this method was successfully employed for the

Authors acknowledge the ASIDE, Govt. of India and University of Agricultural

Sciences, Dharwad, Karnataka, for providing grants for the establishment of

Pesticide Residue Testing and Quality Analysis Laboratory.

There are no conflicts of interest to declare.

*DOI: http://dx.doi.org/10.5772/intechopen.80438*

analysis of real-world grape samples.

**Acknowledgements**

**Conflicts of interest**

**Author details**

Mahadev C. Khetagoudar1

*Gas Chromatographic-Mass Spectrometric Detection of Pesticide Residues in Grapes DOI: http://dx.doi.org/10.5772/intechopen.80438*

sensitivity and selectivity for possible safe identification by the use of Q/q ratio parameter. The limit of detection was lower than the MRL prescribed. Solid-phase extraction with acetonitrile solvent was employed. Finally, the method was successfully validated for two concentrations, viz. 0.02 and 0.05 mg/kg grape sample. The validated method reduces the overall cost of analysis and also offers lowuncertainty measurement. Further, this method was successfully employed for the analysis of real-world grape samples.
