**4. Conclusions**

This chapter describes the qualitative phytochemical screening of two aqueous extracts prepared from dried fruits of sea buckthorn and gooseberry, plants with the important pharmacological properties and rich in nutrients. The qualitative screening consists of standard methods that are able to determine whether a phytochemical is present or not in the aqueous extracts.

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The two aqueous extracts are obtained after a cold infusion at a constant temperature of 4°C for 24 h and are kept at the refrigerator for more than 12 weeks without alteration.

The general screening of carbohydrates revealed that, in the case of sea buckthorn aqueous extract, the solution turns purple-red and a brown precipitate is obtained from gooseberry aqueous extract. Molisch's test revealed that both aqueous extracts contain different classes of carbohydrates. Specific qualitative test for carbohydrates reveal the presence of monosaccharides in gooseberry aqueous extract and of di-, oil- and polysaccharides in both sea buckthorn and gooseberry aqueous extracts.

Alkaloids are absent from both extracts, while cardiac glycosides are present. The test for cysteine gives a positive reaction in the case of sea buckthorn, while ninhydrin test is negative for both aqueous extracts.

The results obtained when aqueous extract from sea buckthorn reacts with Millon reagent is an opalescent orange solution and, in the case of gooseberry aqueous extracts, a red-brownish precipitate is formed, thus confirming that small amounts of proteins and/or amino acids are present in gooseberry.
