**A. Appendix (supplementary materials)**

**Table S1.** Proximate analysis, total phenolic and flavonoid in the seeds of 24 soybean genotypes seeds (on a dry weight basis).

**Sr.** 

**Compound**

**Other name**

Urea 3-Methoxy-propanal

**Nature** Amide Aldehyde

Alkane Amide Heterocyclic

compound

Alcohol

Ether Carboxylic acid

Carboxylic acid

Antimicribial

Antibacterial

Antibacterial

**Activity**

**RT** 3.67 60 3.75 88

3.8 3.86 74

3.92 3.93 4.02 4.17 60

4.24 4.54

4.6 4.73 4.73 4.89 4.92 86

5.3 5.61 94

http://dx.doi.org/10.5772/intechopen.78035

222

101

Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis

123

268

146

146

222

498

104

192

86

**MW**

**no**

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

2-Propenamide

1,2,4-Triazole, 4-(4-methoxybenzylidenamino)-5-

methyl-3-(3,5-dimethylpyrazol-1-yl

L-Galactose, 6-deoxy-

Pyrimidine, 2-methyl-

Cyclotrisiloxane, hexamethyl

2,2-Bioxirane

2-Propanone, 1-(dimethylamino)-

2-Pyridinecarboxylic acid

Silane, triethylmethoxy-

Butanoic acid, 4,4-dithiobis[2-amino-,[S-(R\*,R\*)]

Picolinic acid (Dimethylamino)acetone

Butane1,2:3,4-diepoxy-

Dimethylsiloxane cyclic trimer

2-Methylpyrimidine

6-Deoxyhexose

Acrylamide

Heterocyclic

compound

Heterocyclic

compound

Sugar moiety

Amide Heterocyclic

compound

Preservative

6.39 6.43 71

7.54

310

151

164

Ketone

compound

Heterocyclic

compound

Ethyl(dimethyl)isopropoxysilane

Acetic acid, 2,2-[oxybis(2,1-ethanediyloxy)]bis

(2-[2-(Carboxymethoxy)ethoxy]

ethoxy)acetic acid

Ethyl(dimethyl)silyl isopropyl ether

Methyl trethylsilyl ether

Ether Ether Carboxylic acid

Carboxylic acid

Natural chelator

Acetic acid

1,3-Dioxolane-4-methanol

1-Monolinoleoyglycerol trimethylsilyl ether

Glycerol formal

Acetamide, oxime

1,2-Naphthalenedione, 4 chloro

n-Hexane

Propanal, 3-methoxy

Carbamide


maximum ketone compounds while Giza 111 contributes more in heterocyclic compounds. Some genotypes may have good therapeutic potential and could be served as a potential source in drug wdevelopment as a health supplement. This study also provides a platform for isolating and under-

> **Total fat (g/100 g)**

**Carbohydrate (g/100 g)**

**Total phenolic content mg/g**

**Total flavonoid content mg/g**

standing the properties of each compound for it pharmacological properties.

**Ash (g/100 g)**

Admaril 37.84 4.56 5.27 21.65 30.68 1.30 0.975 Romal-1 40.93 4.79 4.55 20.35 29.38 1.75 1 NARC-2 38.01 4.84 5.79 21.16 30.2 1.50 1.25 Williams 82 38.23 4.97 5.65 22.79 28.36 1.42 1.05 X 32 39.8 4.31 5.54 21.04 29.31 1.25 0.875 Holladay 37.04 4.35 5.55 23.61 29.45 1.35 0.75 Giza 22 39.82 4.45 5.55 21.91 28.27 1.37 0.675 Giza 21 39.84 4.4 5.56 21.72 28.48 1.40 0.8 X2 L 12 38.26 4.26 5.29 21.96 30.23 1.42 1.2 Giza 83 38.29 3.08 5.39 21.42 31.82 1.38 0.925 Crawford 39.43 4.84 5.58 22.38 27.77 1.30 1.125 Giza 35 38.8 3.77 5.49 21.78 30.16 1.32 1.025 X 30 40.05 4.99 5.64 21.78 27.54 1.70 1.0375 Giza 111 36.89 5.34 6.28 22.07 29.42 1.15 1.75 Clark 43.13 5.41 5.77 19.58 26.11 1.35 1.375 3803 40 5.12 5.45 22.94 26.49 1.33 1.625 A − 1 39.01 5.19 4.8 20.69 30.31 1.37 1.45 Ijen 41.7 5.54 5.54 18.66 28.56 1.65 1.25 Indo-black 42.71 5.88 5.36 16.92 29.13 1.65 1.025 Indo-I 42.74 5.88 5.7 19.33 26.35 1.62 1.775 Indo-II 37.87 5.51 5.14 21.17 30.31 1.32 1.375 USA-1 36.89 5.34 5.24 21.34 31.19 1.77 2.125 Indian 36.59 5.43 5.25 20.88 31.85 1.65 1.7625 Chinese 35.98 5.19 5.26 21.06 32.51 1.50 1.35 Argentinian 35.63 5.12 5.3 20.77 33.18 1.37 1.325

**Table S1.** Proximate analysis, total phenolic and flavonoid in the seeds of 24 soybean genotypes seeds (on a dry weight

**A. Appendix (supplementary materials)**

150 Phytochemicals - Source of Antioxidants and Role in Disease Prevention

**Moisture (g/100 g)**

**Genotype name Crude** 

basis).

**protein (g/100 g)**

#### Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis http://dx.doi.org/10.5772/intechopen.78035 151


**Compound**

**Other name** Glutaconic anhydride

Heterocyclic

compound

Heterocyclic

compound

Ketone

compound

Aldehyde

Ketone

compound

Ketone

compound

Sugar moiety

Phenolic

Antimicrobial, antioxidant, antiinflammatory, analgesic

13.83 124

14.24 116 14.24 116

Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis

14.36 98 14.68 126

compound

Ester

Antibacterial

12 12.06 120 12.28 128 12.81 504

120

**Nature**

**Activity**

**RT** 10.75 112 11.54 122

11.86 99

**MW**

**no**

37 38 39 40 41 42 43 44 46 45 formate)

47 48 49 50 51 52 4-one

3,5-Dihydroxy-6-methyl-2,3-dihydro-4H-pyran-

2-Acetyl-2,3,5,6-tetrahydro-1,4-thiazine

1-(3-Thiomorpholinylethanone

Ketone

compound

Heterocyclic compound

Antimicrobial, anti-inflammatory, anti-proliferative

16.61 144 153

5-Hepten-3-one, 5-methyl-

4H-Pyran-4-one,3-hydroxy-2-methyl-

Maltol

Cyclopentane,

(1,1-dimethylethyl)-{Tert-Butylcyclopentane}

1,5-Hexadien-3-ol

Formic acid, 3-methylbut-2-yl ester

1-Butanol,3-methyl-, formate (isopentyl alcohol,

Isopentyl alcohol, formate

Fatty acid ester

Alkene Alkane Heterocyclic

Flavor enhancer

14.78 126 15.09 126

http://dx.doi.org/10.5772/intechopen.78035

15.85 145

compound

Ketone

compound

Antibacterial

Tert-Butylcyclopentane

Antimicrobial activity

Phenol, 2-methoxy-

a-D-Glucopyranoside, O-a-D-glucopyranosyl-(1.

fwdarw.3)-a-D-fructofuranosyl

2,5-Dimethyl-4-hydroxy-3(2H)-furanone

Benzeneacetaldehyde

2,4,6,-Cycloheptatrien-1-one,4-methyl-

2-Pyrrolidinone, 1-methyl

M-Pyrol

1H-Indazole, 4,5, 6, 7-tetrahydro

2H-Pyran-2,6(3H)-dione


**Compound**

**Other name**

**Nature**

**Activity**

**RT** 7.57 7.58 7.67 7.77 86

152 Phytochemicals - Source of Antioxidants and Role in Disease Prevention

7.88 86

7.96 7.98 98

8.02 8.09 98 8.18 90

8.54

9.1 9.33 92

9.74 10.26 114 10.26 238

144

146

104

236

130

108

192

131

**MW**

**no**

21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 37

HEPES[4-(2-Hydroxyethyl)-1-

piperazineethanesuffonic acis)]

Oxirane, [(2-propenyloxy)methyl}-

Propane, 1-(allyloxy)2,3-epoxy-

Heterocyclic

compound

Heterocyclic

compound

1,2,3-Propanetriol

2,4-Dihydroxy-2,5-dimethyl-3(2H)-furan-3-one

Propanedioic acid, Propyl-

2-Propylmalonic acid

Glycerin

Butanoic acid, 4-hydroxy-

Dihydroxyacetone

6-Oxa-bicyclo[3.1.0]hexan-3-one

Pyran-4-carboxylic acid,

4-(4-methoxyphenyl)-tetrahydro-

1,2-Cyclopentanedione

4-Methyl-2-haptanol

Butyrolactone

Oxirane, 2-ethyl-2-methyl

Pyrazine, ethyl-

2H-1-Benzopyran,

3,5,6,8a-tetrahydro-2,5,5,8a-tetramethyl-,(2S-cis)-

Acetic acid, 2-(dimethylamino)ethyl ester

Dimethylaminoethanol acetate

Edulan II Ethylpyrazine Butane, 1,2-epoxy-2-methyl

Heterocyclic

compound

Ketone

compound

Alcohol Ketone

Antioxidant

compound

Heterocyclic compound

Ketone

compound

2-Propanone, 1,3-dihydroxy-

Ketone

compound

Carboxylic acid

Carboxylic acid

Alcohol Ketone

compound

Ester Heterocyclic

compound

Heterocyclic

compound

#### Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis http://dx.doi.org/10.5772/intechopen.78035 153


**Compound**

**Other name**

**Nature**

**Activity**

**RT**

**MW**

**no**

71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 **Table S2.**

Phthalic acid, butyl undecyl ester

1,2-Benzenedicarboxylic acid, dibutyl ester

Methyl 10 trans, 12-cis-octadecadienoate

List and basic features of identified phytocomponents in the methanolic extract of soybean genotypes by GCMS analysis.

Dibutyl phthalate

5,8, 11-Heptadecatriynoic acid methyl ester

Hexadecanoic acid, methyl ester

Palmitic acid, methyl ester

Ester Ester Ester Ester Ester

Plasticizer, antimicrobial, antifouling

47.37 278 48.53 294 155

Antioxidant, flavor,

hypocholesterolemic, nematicide

5-Tert.butyloxy

carboxamido-2,3,3-trimethyl-3H-indole

Phthalic acid, hex-3-yl-isobutyl ester

Dodecyl acrylate

Cyclopenta [1,3][cyclopropa [1,2]cyclohepten-3(3ah)one, 1,2,3b,6,7,8-hexahydro-6,6-dimethyl-

Tert-butyl 2, 3,3-trimethyl-3H-indole-

Heterocyclic compound

Ester

5-ylcarbamate

n-Lauryl acrylate

2,6-Diisopropylnapthalene

3-O-methyl-d-glucose

a-D-Galactopyranoside, methyl

Galactopyranoside, methyl, a-D-

3-O-methylhexose

Sugar moiety Sugar moiety

Hetercyclic

compound

Ester Ketone compound

Preservative

Preservative

a-Methyl-D-mannopyranoside

3,5-Dimethoxyacetophenone

Pentanoic acid, 2,2-4-trimethyl-3-

carboxyisopropyl,isobutyl ester

3,4-0-Isopropylidene-d-galactose

3,4,0-(1-Methylethylidene)

hexopyranose

Benzoic acid, 4-ethoxy-, ethyl ester

5-Tert-butyl-1,2,3-benzenetriol

5-Tert-butylpyrogallol

Phenolic

Antioxidant, antiseptic antibacterial,

31.91 182 32.12 194 32.35 220 32.97 286

anti-dermatitic fungicide, pesticide

compound

Ester Sugar moiety

Ester Ketone

antioxidant

33.65 180

compound

Sugar moiety

Preservative

34.35 194 34.61 194 37.68 194 37.71 212

38.17 240 40.31 190

41.6 42.4 46.13 270

46.2 47.36 376

272

http://dx.doi.org/10.5772/intechopen.78035

306

274

Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis

Preservative


**Compound**

**Other name** 1-Methyl-2-oxoethyl benzoate

Aldehyde Carboxylic acid

Heterocyclic

compound

**Nature**

**Activity**

**RT** 16.69 178 16.76 122 16.95 102 18.26 236 19.29 162

154 Phytochemicals - Source of Antioxidants and Role in Disease Prevention

**MW**

**no**

53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70

Phenol, 2,4-bis(1,1-dimethylethyl)-

Phenol, 2,4-di-tert-butyl-

1-Undecanol alcohol

Phenol,2,6-bis(1,1-dimethylethyl)-4-methyl-

Phenol, 2,6-dimethoxy-

2-Acetamido-2-deoxy-d-mannolactone

Pyrogallol 1,3-dimethyl ether

Undecyl alcohol

Butylated hydroxytoluene

Phenolic

compound

Alcohol Phenolic

Antimicrobial, antioxidant, antiinflammatory, analgesic

Antimicrobial, antioxidant, antiinflammatory, analgesic

31.19 206

compound

Phenolic

compound

(p-Hydroxyphenyl)glyoxal

2-Methoxy-4-vinylphenol

Isosorbide

1,2-Benzenediol,3-methoxy

Benzeneacetaldehyde, 3-methyl

m-Tolualdehyde

Pyrocatechol, 3-methoxy

D-Glucitol, 1,4,3,6-dianhydro

phenol, 4-ethenyl-2-methoxy-

Benzeneacetaldehyde,

4-hydroxy-a-0x0

Phenolic

Antimicrobial, antioxidant, antiinflammatory, analgesic

compound

Aldehyde Sugar moiety

Anti-bacterial

Antimicrobial, antioxidant, antiinflammatory, analgesic

29.83 172 30.99 220

24.8 24.99 154

217

Antibacterial

23.71 150

Alcohol

Benzofuran, 2,3-dihydro

Benzene, (ethenyloxy)-

Benzaldehyde, 3,4-dimethyl-

Phenol, 4-ethenyl-, acetate

Pentanoic acid, 2-isopropoxyphenyl ester

2-Isopropoxyphenyl pentanoate

4-Vinylphenyl acetate

3,4-dimethylbenzaldehyde

Ether, phenyl vinyl

Coumaran

Ester Phenolic

Antimicrobial, antioxidant, antiinflammatory, analgesic

compound

Aldehyde

Ether Heterocyclic

Antihelminthic, anti-inflammatory,

compound

Aldehyde

Phenolic

Antioxidant

compound

Antimicrobial

20.34 120 21.01 140 22.61 146 23.35 150

anti-diarrhoeal

Antibacterial

19.3 19.31 120 20.16 120

134

N-aminomorpholine

4-Aminomorpholine

Benzoic Acid

Propanal, 2-(benzoyloxy)-,®

**Table S2.** List and basic features of identified phytocomponents in the methanolic extract of soybean genotypes by GCMS analysis.

Phytochemical Profiling of Soybean (*Glycine max* (L.) Merr.) Genotypes Using GC-MS Analysis http://dx.doi.org/10.5772/intechopen.78035 155
