**3. Gene therapy**

When developing a gene therapy-based medicinal product, one should keep in mind that no active substance will become a drug product unless it can be properly formulated and administered. Compared to more conventional small molecules, gene therapy strategies based on nucleic acids are faced with new constraints linked to their chemical nature, the size of the molecule, and the coding sequence composition.

Different pharmaceutical designs for gene therapy could be considered. In the context of CM and CHF, we will focus more precisely on how to restore the functional allele in the context of inherited CM and more broadly how to restore or improve myocardial contractility.

#### **3.1. Inherited CM**

In the context of inherited CM, most frequently a monogenic transmission profile has been identified, expression profiles being either dominant or more frequently recessive. Sometimes CMs are part of a larger clinical context of a systemic myopathy, but usually cardiac and neuromuscular disorders are not proportional and thus would need separate and specific treatment, even if the genetic origin can be unique.

Considering the situation of the single causative gene acting in a recessive mode, it might be tempting to restore a normal phenotype through addition of a functional allele. So conceptually at this level, gene therapy is mainly derived from gene transfer techniques largely used in cell biology by introducing an exogenous sequence of nucleic acids into a eukaryotic cell to express new information on these cells. Over time, several independent laboratories have demonstrated that the concept of transferring an exogenous gene into the myocardium of mammals was possible, leading to the expression of a new protein not coded by the intrinsic genes. However, to transform a laboratory technique of gene transfer into a therapeutic option, additional steps had to be considered. To assess the therapeutic capabilities of gene transfer, protein expression cannot be the primary outcome measure. More subtle integration of the pathophysiology of each CM is mandatory. Considering inherited diseases where a certain phenotypic latency exists, it was possible to demonstrate that gene transfer of a functional allele was able to delay the onset of an overt CM. More generally, when designing a gene therapy strategy, one should consider whether the defective gene should be rescued or whether other genes involved in heart failure development and progression should be targeted.

## **3.2. Preclinical models of CM**

ill patients. Thereby, in terms of therapeutic efficiency and risk/benefit ratio, initial evaluations will refer to outcomes of surgical procedures. The gene therapist should be aware of the

So far, none of the existing treatments have definitively changed the fate of CM and CHF. There is thus space for new drug developments and gene therapy might help to solve some of the intrinsic hurdles of CM and CHF. For instance, none of the existing treatments really change

Advances in gene transfer vectors, development of new vector delivery methods, and discovery of new gene targets continue to fuel our motivation to use this approach in routine

When developing a gene therapy-based medicinal product, one should keep in mind that no active substance will become a drug product unless it can be properly formulated and administered. Compared to more conventional small molecules, gene therapy strategies based on nucleic acids are faced with new constraints linked to their chemical nature, the size of the

Different pharmaceutical designs for gene therapy could be considered. In the context of CM and CHF, we will focus more precisely on how to restore the functional allele in the context of

In the context of inherited CM, most frequently a monogenic transmission profile has been identified, expression profiles being either dominant or more frequently recessive. Sometimes CMs are part of a larger clinical context of a systemic myopathy, but usually cardiac and neuromuscular disorders are not proportional and thus would need separate and specific

Considering the situation of the single causative gene acting in a recessive mode, it might be tempting to restore a normal phenotype through addition of a functional allele. So conceptually at this level, gene therapy is mainly derived from gene transfer techniques largely used in cell biology by introducing an exogenous sequence of nucleic acids into a eukaryotic cell to express new information on these cells. Over time, several independent laboratories have demonstrated that the concept of transferring an exogenous gene into the myocardium of mammals was possible, leading to the expression of a new protein not coded by the intrinsic genes. However, to transform a laboratory technique of gene transfer into a therapeutic option, additional steps had to be considered. To assess the therapeutic capabilities of gene transfer, protein expression cannot be the primary outcome measure. More subtle integration of the pathophysiology of each CM is mandatory. Considering inherited diseases where a certain phenotypic latency exists, it was possible to demonstrate that gene transfer of a functional allele

inherited CM and more broadly how to restore or improve myocardial contractility.

myocardial contractility without excessively increasing oxygen consumption.

competing therapeutic strategies.

174 In Vivo and Ex Vivo Gene Therapy for Inherited and Non-Inherited Disorders

bedside care [25,26].

**3. Gene therapy**

**3.1. Inherited CM**

molecule, and the coding sequence composition.

treatment, even if the genetic origin can be unique.

Delta-sarcoglycan (dSG)-deficient hamsters represent a well-characterized genetically determined model of a CM. Phenotypically these animals develop a dilated cardiomyopathy (DCM) with terminal heart failure over a rather short time span as they die as mid-aged adults [27,28]. From the perspective of gene therapy they represent a very useful model. Beyond the clear phenotype, the causal genetic mutation is known, coding sequences are readily available, and transmission is autosomal recessive. A single allele correction can correct or at least clearly improve the phenotype when administered in young animals [29]. Some authors observed even more than a simple phenotypic rescue as the lifespan of these animals seemed to increase [30,31].

However, unlike dSG, coding sequences of some normal alleles can be very extensive, the most extreme case being dystrophin with a full-length cDNA of more than 11 kb. Several strategies can be considered. Given some structural specificities, reengineering of the active pharmaceutical ingredient (API) can be performed while retaining therapeutic potential. Thus, gene therapy should not simply be considered as a substitution of defective alleles. Hence, truncated forms of dystrophin have proven to alleviate pathologic phenotypes in several experiment models [32,33].

Similarly, it was possible to show that editing the intrinsic messenger RNA can lead to coding of a functional protein. Exon skipping is used to restore the reading frame within a gene. The mechanism behind exon skipping is a mutation-specific antisense oligonucleotide. An antisense oligonucleotide is a synthesized short nucleic acid polymer that will bind to the mutation site in the pre-messenger RNA to induce exon skipping. In the context of Duchenne muscular dystrophy (DMD) the genetic mutation that leads to Becker muscular dystrophy (BMD) is an in-frame deletion. Exon skipping can induce the expression of a truncated but functional dystrophin protein and thus switch the phenotype of some DMD-type mutations to the phenotype of a BMD-type mutation [34,35].

Multiple arguments in favour of the feasibility of cardiac gene therapy have been generated over time. However, these experiments raise new questions. Most non-clinical studies were carried out on well-characterized model-rescuing defective genotypes and avoiding or delaying the development of a pathological phenotype. So referring to clinical settings, this mimics mainly presymptomatic situations.

In this setting, gene therapy would basically be a prophylactic option to avoid development of a pathological phenotype, but are we ready for a gene therapy that would be mainly preventive? What would an acceptable risk/benefit ratio be in that case? Ideally in the context of preventive medicine, gene therapy of an inherited monogenic disorder should by homologous recombination correct most if not all of the affected cells without any off-target adverse effects. Gene therapy has not yet reached this level of maturity. Nevertheless, this does not mean that such options cannot be tested in the future.

#### **3.3. Heart failure**

If gene therapy offers the possibility to interfere intimately and subtly with the molecular pathways governing the pathological processes, then introducing genetic material into cells should be able not only to compensate for abnormal genes but also to influence pathways involved in the development and progression of the disease.

Besides structural changes of the myocardium, influencing cardiac function could represent further targets for gene therapy strategies. Taking advantage of the well-known hamster model, one can reformulate the clinical question as the progressive decline of contractility and development of patent heart failure. We considered animals at an early symptomatic stage to mimic as closely as possible a clinically relevant situation. We compared the efficiency of administering either a functional cDNA of dSG (rescuing the causal genetic defect) or a cDNA coding for a Ca2+-handling protein, for instance SERCA2a. SERCA2a holds a key role in the development and progression of heart failure, so after the initial work by Schwartz and coworkers, it was rather obvious to test its therapeutic potential [38–40]. Briefly, we could demonstrate that from a therapeutic perspective at a clinical stage of patent heart failure, great benefits could be obtained by targeting cardiomyocyte Ca2+ homeostasis through SERCA2a gene expression than rescuing the initial causative genetic defect [41]. These findings as well as results from several other labs strongly support the strategy of cardiac gene therapy for heart failure based on restoring appropriate Ca2+ handling [42–44]. At this stage, one should cite the pioneering work led by Hajjar that led to a clinical trial (CUPID) using an expression cassette coding for SERCA2a [45]. This phase IIa study retained some intrinsic limitations due to the low number of patients. Therefore, a larger phase IIb study (CUPID2) with a double-blinded, placebo-controlled, and randomized event-driven schema and based on multinational, multicenter recruitment (n = 250) was needed to confirm the initial results described in the CUPID1 study. This phase IIb CUPID2 trial did not meet its primary and secondary endpoints. Nevertheless, multiple useful data were generated by this clinical trial. Gene therapy is a realistic therapeutic strategy in the field of CMs. Patient selection is always a difficult task in those very innovative steps, but the trial allowed refining the criteria. It also became apparent how important formulation of the API and administration are. Before discussing these aspects, one should acknowledge the research done by K. Hammond and coworkers that explored the therapeutic potential of adenylyl-cyclase type 6 (AC6) [46–48]. These authors showed that activation of cardiac AC6 expression improves impaired function of aged hearts through improved calcium uptake. AC6 determines cAMP formation. However, favourable effects on cardiac function through abrogation of hypertrophy, increased cell survival, and improved calcium handling appear to be cAMP independent. The main goal of the trial based on AC6 administration in CHF patients is to evaluate the safety and efficacy of human AC6 gene product as a new therapeutic option. To reach this goal, 56 patients were (or have been if the trial is still ongoing - please check) included in this study, in which gene delivery was based on a drug formulation where human AC6 was

Gene Therapy for Cardiomyopathies http://dx.doi.org/10.5772/intechopen.80478

Expression of a peptide inhibitor of GRK2 (βARKct) can improve the contractility of failing

Inhibition with antimiR-34a/antimiR-34 has emerged as a promising therapeutic strategy, as silencing of miR-34a attenuates cardiac dysfunction in a setting of moderate HCM. However, the beneficial effect does not appear in severe HCM [49]. Thus, it appears important to make appropriate staging of the clinical symptomatology, hence the cardiac phenotype. Therapies that inhibit miR-34a alone may have limited potential in settings of established cardiac pathology [50]. For instance, miR-133, which is enriched in cardiac and skeletal muscle, is involved in cell specification, differentiation, and development. Furthermore, miR-a33 is

myocardium and promote reverse remodelling of the left ventricle.

carried by an adenovirus serotype 5.

In the context of inherited CMs, we postulate that several steps might occur sequentially. The causative genetic defect can be inherited or be a neo-mutation and will trigger a cascade of deleterious effects that will lead to the appearance of a patent cardiac disease. Progressively the genetic features of heart failure will dominate and one might consider that at a later stage these changes will be almost independent of the original genetic defect. Moreover, we might consider that genetic modifications at this stage are similar to those that occur in the context of acquired CM. While any disease is a potential target for gene therapy, some treatments are easier to achieve in the clinic. To test this working hypothesis experimentally, we used mainly the same dSG-deficient hamster model. However, to mimic symptomatic disease, animals were included at a later age.

Many molecular targets could be considered at this level, but several candidates might be more prominent in the present context. We have already discussed the case of gene addition of a functional allele in the context of an autosomal recessive disorder. The candidate gene will of course depend on a proper identification of the genetic disease involved in the CM. On the other side, considering the heart failure phenotype as such, multiple options appear.

Rather basically, we evaluated genes preserving myocardial structure. In the experimental setting that we considered as a model, it is known that hamsters develop a DCM. However, in some substrains carrying the very same mutation but in slightly different genetic backgrounds, animals can develop firstly a phase of HCM and have a less severe phenotype. So the question became, can we mimic this feature by introducing exogenous genetic information?

Physiologic remodelling is a compensatory change in the dimensions and function of the heart in response to physiologic stimuli such as exercise and pregnancy. The remodelling process frequently includes increases in myocardial mass. The heart can respond to environmental stimuli by growth (increased myocardial mass) or shrinkage (atrophy) with a rather large dynamic range. Remodelling is induced by changes in gene expression, which, in turn, alter the expression of key regulatory proteins, the distribution and function of subcellular organelles, the size and morphology of individual cells, the properties of the extracellular matrix, and ultimately those of the entire organ. IGF-1 is a key player in this context and prior to developing a gene therapy option we could demonstrate that administration of a recombinant IGF-1 protein can exert several beneficial effects of the cardiac phenotype of dSG-deficient hamsters [36,37]. However, a recombinant protein with pleiotropic effects will inevitably lead to extracardiac adverse effects. Therefore, a gene therapy option might offer a more targeted treatment, especially when associating local delivery with tissue-specific regulatory sequences. IGF-1 served as a role model to highlight some of the innovative differences between gene therapy and conventional treatments, but of course other APIs could be developed along a similar strategy. Various pathophysiological processes could be targeted, such as interfering with the fibrosis–cell death axis and promoting cell survival.

Besides structural changes of the myocardium, influencing cardiac function could represent further targets for gene therapy strategies. Taking advantage of the well-known hamster model, one can reformulate the clinical question as the progressive decline of contractility and development of patent heart failure. We considered animals at an early symptomatic stage to mimic as closely as possible a clinically relevant situation. We compared the efficiency of administering either a functional cDNA of dSG (rescuing the causal genetic defect) or a cDNA coding for a Ca2+-handling protein, for instance SERCA2a. SERCA2a holds a key role in the development and progression of heart failure, so after the initial work by Schwartz and coworkers, it was rather obvious to test its therapeutic potential [38–40]. Briefly, we could demonstrate that from a therapeutic perspective at a clinical stage of patent heart failure, great benefits could be obtained by targeting cardiomyocyte Ca2+ homeostasis through SERCA2a gene expression than rescuing the initial causative genetic defect [41]. These findings as well as results from several other labs strongly support the strategy of cardiac gene therapy for heart failure based on restoring appropriate Ca2+ handling [42–44]. At this stage, one should cite the pioneering work led by Hajjar that led to a clinical trial (CUPID) using an expression cassette coding for SERCA2a [45]. This phase IIa study retained some intrinsic limitations due to the low number of patients. Therefore, a larger phase IIb study (CUPID2) with a double-blinded, placebo-controlled, and randomized event-driven schema and based on multinational, multicenter recruitment (n = 250) was needed to confirm the initial results described in the CUPID1 study. This phase IIb CUPID2 trial did not meet its primary and secondary endpoints. Nevertheless, multiple useful data were generated by this clinical trial. Gene therapy is a realistic therapeutic strategy in the field of CMs. Patient selection is always a difficult task in those very innovative steps, but the trial allowed refining the criteria. It also became apparent how important formulation of the API and administration are. Before discussing these aspects, one should acknowledge the research done by K. Hammond and coworkers that explored the therapeutic potential of adenylyl-cyclase type 6 (AC6) [46–48]. These authors showed that activation of cardiac AC6 expression improves impaired function of aged hearts through improved calcium uptake. AC6 determines cAMP formation. However, favourable effects on cardiac function through abrogation of hypertrophy, increased cell survival, and improved calcium handling appear to be cAMP independent. The main goal of the trial based on AC6 administration in CHF patients is to evaluate the safety and efficacy of human AC6 gene product as a new therapeutic option. To reach this goal, 56 patients were (or have been if the trial is still ongoing - please check) included in this study, in which gene delivery was based on a drug formulation where human AC6 was carried by an adenovirus serotype 5.

**3.3. Heart failure**

were included at a later age.

If gene therapy offers the possibility to interfere intimately and subtly with the molecular pathways governing the pathological processes, then introducing genetic material into cells should be able not only to compensate for abnormal genes but also to influence pathways

In the context of inherited CMs, we postulate that several steps might occur sequentially. The causative genetic defect can be inherited or be a neo-mutation and will trigger a cascade of deleterious effects that will lead to the appearance of a patent cardiac disease. Progressively the genetic features of heart failure will dominate and one might consider that at a later stage these changes will be almost independent of the original genetic defect. Moreover, we might consider that genetic modifications at this stage are similar to those that occur in the context of acquired CM. While any disease is a potential target for gene therapy, some treatments are easier to achieve in the clinic. To test this working hypothesis experimentally, we used mainly the same dSG-deficient hamster model. However, to mimic symptomatic disease, animals

Many molecular targets could be considered at this level, but several candidates might be more prominent in the present context. We have already discussed the case of gene addition of a functional allele in the context of an autosomal recessive disorder. The candidate gene will of course depend on a proper identification of the genetic disease involved in the CM. On the other side, considering the heart failure phenotype as such, multiple options appear.

Rather basically, we evaluated genes preserving myocardial structure. In the experimental setting that we considered as a model, it is known that hamsters develop a DCM. However, in some substrains carrying the very same mutation but in slightly different genetic backgrounds, animals can develop firstly a phase of HCM and have a less severe phenotype. So the question became, can we mimic this feature by introducing exogenous genetic information?

Physiologic remodelling is a compensatory change in the dimensions and function of the heart in response to physiologic stimuli such as exercise and pregnancy. The remodelling process frequently includes increases in myocardial mass. The heart can respond to environmental stimuli by growth (increased myocardial mass) or shrinkage (atrophy) with a rather large dynamic range. Remodelling is induced by changes in gene expression, which, in turn, alter the expression of key regulatory proteins, the distribution and function of subcellular organelles, the size and morphology of individual cells, the properties of the extracellular matrix, and ultimately those of the entire organ. IGF-1 is a key player in this context and prior to developing a gene therapy option we could demonstrate that administration of a recombinant IGF-1 protein can exert several beneficial effects of the cardiac phenotype of dSG-deficient hamsters [36,37]. However, a recombinant protein with pleiotropic effects will inevitably lead to extracardiac adverse effects. Therefore, a gene therapy option might offer a more targeted treatment, especially when associating local delivery with tissue-specific regulatory sequences. IGF-1 served as a role model to highlight some of the innovative differences between gene therapy and conventional treatments, but of course other APIs could be developed along a similar strategy. Various pathophysiological processes could be targeted,

such as interfering with the fibrosis–cell death axis and promoting cell survival.

involved in the development and progression of the disease.

176 In Vivo and Ex Vivo Gene Therapy for Inherited and Non-Inherited Disorders

Expression of a peptide inhibitor of GRK2 (βARKct) can improve the contractility of failing myocardium and promote reverse remodelling of the left ventricle.

Inhibition with antimiR-34a/antimiR-34 has emerged as a promising therapeutic strategy, as silencing of miR-34a attenuates cardiac dysfunction in a setting of moderate HCM. However, the beneficial effect does not appear in severe HCM [49]. Thus, it appears important to make appropriate staging of the clinical symptomatology, hence the cardiac phenotype. Therapies that inhibit miR-34a alone may have limited potential in settings of established cardiac pathology [50]. For instance, miR-133, which is enriched in cardiac and skeletal muscle, is involved in cell specification, differentiation, and development. Furthermore, miR-a33 is downregulated during cardiac hypertrophy. Specific knockdown of miR-133 via antisense targeting can be sufficient for inducing cardiac hypertrophy and reinduction of the foetal gene programme [51]. In the context of DCM it might be useful to induce a compensatory mechanism by reengaging the foetal gene programme. The miR-22 should also be considered as a critical regulator of cardiomyocyte hypertrophy and cardiac remodelling [52]. Systemic inhibition of miR-21 has proven effective against myocardial fibrosis and dysfunction [53].

the presence of preexisting neutralizing antibodies precludes a number of patients from participation. Furthermore, immune elimination of infected cells often limits gene expression *in vivo*. Readministration remains a major challenge, because single shot solutions are counterintuitive in the era of precision or personalized medicine. Further work is therefore needed to improve viral vectors, more specifically, developing stealthier AAV vectors with the aim of optimizing vector–host interactions [59–61]. Low-grade immune stimulation by the vector system appears as an important point in terms of drug development to avoid severe adverse

Gene Therapy for Cardiomyopathies http://dx.doi.org/10.5772/intechopen.80478

Optimal gene therapy vectors should meet the following criteria: retaining the safety profile of naked DNA while displaying increased efficiency and decreased variability. From this perspective, non-viral methods of transfection present certain advantages such as relative ease of large-scale production, low risk of an adaptive immune response, versatility, and high safety

Most of the non-viral vectors currently developed are based on polycationic molecules, which form interpolyelectrolyte complexes with the polyanionic nucleic acids. The complexes obtained generally allow for (1) efficient condensation of nucleic acids into small particles, (2) protection against degradation from nucleases, and (3) promotion of cellular uptake. These non-viral vectors usually consist of cationic lipids/liposomes (lipoplexes), cationic polymers

Among these, vectors based on lipids are especially attractive due to the biocompatibility and biodegradability of lipids and phospholipids [64]. However, the toxicity displayed by cationic lipids, as well as the rapid clearance of positively charged lipoplexes, hampers further use *in vivo* of first-generation lipoplexes [64]. Coating the surface of lipoplexes, with hydrophilic polymers such as polyethylene glycol (PEG) can efficiently decrease their toxicity while increasing their circulation half-life [65]. Nonetheless, PEGylated lipoplexes often display reduced transfection efficiency due to diminished cellular uptake and can trigger anti-PEG IgM production, thus leading to accelerated blood clearance after readministration [66].

The tremendous diversity of shape, composition, and charge ratio of cationic polymers is a great asset when formulating polyplexes. Cationic polymers, which have been most widely used for cardiac gene delivery, include polyethylenimine, poly-(l-lysine), and dendrimers [67]. Despite their capacity to efficiently condense nucleic acids while preventing their degradation by nucleases and improving endosomal escape, the resulting *in vivo* gene expression

Although non-viral vectors have dramatically improved over the past decades, they remain underrepresented for cardiac gene delivery. Further improvements to increase transfection

From this perspective, polymers displaying few or no positive charges could be the much needed formulation for cardiac gene therapy [69]. Poloxamers, which are non-ionic amphiphilic

remains too low and, for some of them, cytotoxic effects are detected [62,67,68].

efficiency while reducing their cytotoxicity are much needed.

(polyplexes), or a combination of both lipids and polymers (lipopolyplexes) [62,63].

reactions.

profile.

**3.5. Non-viral vectors for cardiac gene therapy**

Substantial advances in the understanding of the cellular and molecular basis of CMs and CHF highlight the potential utility of gene therapy as a novel therapeutic approach. However, successful clinical translation is still limited by the lack of safe, efficient, and selective delivery systems.

Naked DNA has remained the preferred method of gene delivery to the myocardium and has been explored extensively in clinical trials mainly in the setting of ischemic heart disease. The results from these trials have demonstrated efficacy with regards to secondary endpoints of reduced symptomatology, but have failed to establish significant increase in angiogenesis or an improvement in myocardial function [54].

#### **3.4. Viral vectors for cardiac gene therapy**

Viruses have evolved to become highly efficient at nucleic acid delivery to specific cell types while mostly avoiding immunosurveillance by an infected host. Several types of viruses, including retrovirus, adenovirus, adeno-associated virus (AAV), and herpes simplex virus, have been modified in the laboratory for use in gene therapy applications. Adenoviruses are an efficient gene delivery system in a broad range of cell and tissue types. However, the adverse immune reactions represent an important drawback for its development. Over time, multiple viral vector systems have been tested, but more recently AAVs have become most popular. AAVs are nonenveloped parvoviruses, which can rather easily be engineered to deliver DNA cargo to target cells. AAV vectors have demonstrated good potential for in vivo delivery of genetic material into various cells, thus appearing as a vector of choice for different therapeutic applications beyond cardiac diseases. Nevertheless, and even if some promising clinical outcomes have been reported, the current potential of viral vectors for gene therapy still faces significant restrictions, largely due to manufacturing challenges, including the absence of an efficient and scalable platform purification process [25, 55–58]. At least in the setting of murine models, AAV1, AAV6, AAV8, and AAV9 have been identified as the most cardiotropic serotypes after systemic delivery.

The concept of gene therapy seems straightforward, but this is clearly an oversimplification, and numerous problems and risks exist that prevent gene therapy using viral vectors. Due to the structure of the viral particles, AAV vectors retain limited DNA cargo capacity necessitating the need to optimize the therapeutic sequence. Multiple cells can be infected by AAVs, but overall the transduction efficiency remains low leading to increased multiplicities of infection, hence putting greater pressure on large-scale vector production. Moreover, AAVs' tropism lacks cell-type selectivity resulting in off-target transduction. Regulation of the transgene expression remains difficult and frequently results in decreased expression efficiency. Hence, to achieve optimal clinical outcome, high vector doses are required, but the presence of preexisting neutralizing antibodies precludes a number of patients from participation. Furthermore, immune elimination of infected cells often limits gene expression *in vivo*. Readministration remains a major challenge, because single shot solutions are counterintuitive in the era of precision or personalized medicine. Further work is therefore needed to improve viral vectors, more specifically, developing stealthier AAV vectors with the aim of optimizing vector–host interactions [59–61]. Low-grade immune stimulation by the vector system appears as an important point in terms of drug development to avoid severe adverse reactions.

#### **3.5. Non-viral vectors for cardiac gene therapy**

downregulated during cardiac hypertrophy. Specific knockdown of miR-133 via antisense targeting can be sufficient for inducing cardiac hypertrophy and reinduction of the foetal gene programme [51]. In the context of DCM it might be useful to induce a compensatory mechanism by reengaging the foetal gene programme. The miR-22 should also be considered as a critical regulator of cardiomyocyte hypertrophy and cardiac remodelling [52]. Systemic inhibition of miR-21 has proven effective against myocardial fibrosis and dysfunction [53].

Substantial advances in the understanding of the cellular and molecular basis of CMs and CHF highlight the potential utility of gene therapy as a novel therapeutic approach. However, successful clinical translation is still limited by the lack of safe, efficient, and selective delivery

Naked DNA has remained the preferred method of gene delivery to the myocardium and has been explored extensively in clinical trials mainly in the setting of ischemic heart disease. The results from these trials have demonstrated efficacy with regards to secondary endpoints of reduced symptomatology, but have failed to establish significant increase in angiogenesis or

Viruses have evolved to become highly efficient at nucleic acid delivery to specific cell types while mostly avoiding immunosurveillance by an infected host. Several types of viruses, including retrovirus, adenovirus, adeno-associated virus (AAV), and herpes simplex virus, have been modified in the laboratory for use in gene therapy applications. Adenoviruses are an efficient gene delivery system in a broad range of cell and tissue types. However, the adverse immune reactions represent an important drawback for its development. Over time, multiple viral vector systems have been tested, but more recently AAVs have become most popular. AAVs are nonenveloped parvoviruses, which can rather easily be engineered to deliver DNA cargo to target cells. AAV vectors have demonstrated good potential for in vivo delivery of genetic material into various cells, thus appearing as a vector of choice for different therapeutic applications beyond cardiac diseases. Nevertheless, and even if some promising clinical outcomes have been reported, the current potential of viral vectors for gene therapy still faces significant restrictions, largely due to manufacturing challenges, including the absence of an efficient and scalable platform purification process [25, 55–58]. At least in the setting of murine models, AAV1, AAV6, AAV8, and AAV9 have been identified as the most cardiotropic serotypes after systemic delivery.

The concept of gene therapy seems straightforward, but this is clearly an oversimplification, and numerous problems and risks exist that prevent gene therapy using viral vectors. Due to the structure of the viral particles, AAV vectors retain limited DNA cargo capacity necessitating the need to optimize the therapeutic sequence. Multiple cells can be infected by AAVs, but overall the transduction efficiency remains low leading to increased multiplicities of infection, hence putting greater pressure on large-scale vector production. Moreover, AAVs' tropism lacks cell-type selectivity resulting in off-target transduction. Regulation of the transgene expression remains difficult and frequently results in decreased expression efficiency. Hence, to achieve optimal clinical outcome, high vector doses are required, but

systems.

an improvement in myocardial function [54].

178 In Vivo and Ex Vivo Gene Therapy for Inherited and Non-Inherited Disorders

**3.4. Viral vectors for cardiac gene therapy**

Optimal gene therapy vectors should meet the following criteria: retaining the safety profile of naked DNA while displaying increased efficiency and decreased variability. From this perspective, non-viral methods of transfection present certain advantages such as relative ease of large-scale production, low risk of an adaptive immune response, versatility, and high safety profile.

Most of the non-viral vectors currently developed are based on polycationic molecules, which form interpolyelectrolyte complexes with the polyanionic nucleic acids. The complexes obtained generally allow for (1) efficient condensation of nucleic acids into small particles, (2) protection against degradation from nucleases, and (3) promotion of cellular uptake. These non-viral vectors usually consist of cationic lipids/liposomes (lipoplexes), cationic polymers (polyplexes), or a combination of both lipids and polymers (lipopolyplexes) [62,63].

Among these, vectors based on lipids are especially attractive due to the biocompatibility and biodegradability of lipids and phospholipids [64]. However, the toxicity displayed by cationic lipids, as well as the rapid clearance of positively charged lipoplexes, hampers further use *in vivo* of first-generation lipoplexes [64]. Coating the surface of lipoplexes, with hydrophilic polymers such as polyethylene glycol (PEG) can efficiently decrease their toxicity while increasing their circulation half-life [65]. Nonetheless, PEGylated lipoplexes often display reduced transfection efficiency due to diminished cellular uptake and can trigger anti-PEG IgM production, thus leading to accelerated blood clearance after readministration [66].

The tremendous diversity of shape, composition, and charge ratio of cationic polymers is a great asset when formulating polyplexes. Cationic polymers, which have been most widely used for cardiac gene delivery, include polyethylenimine, poly-(l-lysine), and dendrimers [67]. Despite their capacity to efficiently condense nucleic acids while preventing their degradation by nucleases and improving endosomal escape, the resulting *in vivo* gene expression remains too low and, for some of them, cytotoxic effects are detected [62,67,68].

Although non-viral vectors have dramatically improved over the past decades, they remain underrepresented for cardiac gene delivery. Further improvements to increase transfection efficiency while reducing their cytotoxicity are much needed.

From this perspective, polymers displaying few or no positive charges could be the much needed formulation for cardiac gene therapy [69]. Poloxamers, which are non-ionic amphiphilic block copolymers, were first reported by Lemieux et al. [70] as efficient formulations for muscle gene delivery. Contrary to polycationic molecules, these delivery systems do not condense DNA into small particles and display no or weak interactions with nucleic acids [69,71]. Direct intramyocardial injection of poloxamer/DNA formulations showed no toxic effect towards the myocardium although gene expression remained limited and restricted to the injection site [71]. To increase the diffusion of poloxamer/DNA formulations into the myocardium, further experiments conducted *in vivo* on larger animals, through a clinically relevant administration route, were performed. As seen in **Figure 3**, this resulted in similar gene expression rate compared to that of the same transgene delivered using an AAV1 vector. To provide more insight into poloxamer-based delivery systems, further studies addressing their mechanism of action as well as experiments evaluating the possibility to readminister these formulations should be carried out.

local gene transfer by a topical administration. Proof-of-concept studies have shown the efficiency of this approach in atrial fibrillation. Strategies based on ultrasound-targeted microbubble destruction could be a promising method for gene delivery [73]. Microbubbles are small (<5 μm) gas-filled voids that are generally stabilized by phospholipids or synthetic polymers. The use of microbubbles as gene vectors is based on the paradigm that destruction of the DNA-loaded microbubbles by ultrasounds will result in local transduction and still spare non-target areas. Percutaneous antegrade coronary injections are among the least invasive cardiac selective gene delivery methods and are rather broadly available. Intracoronary delivery allows diffuse transduction throughout the myocardium, but as such it is a highly inefficient process. However, dense regional gene transfer (>80% of myocytes in the target territory) is possible. Pharmacological manipulations to induce vasodilation and maximize vascular permeability in a specific coronary perfusion territory can greatly improve transfection efficiency [74]. Given the high perfusion velocity and the submaximal extent of the vascular bed, one has to maximize the duration of vector exposure to the local vasculature while minimizing the systemic distribution. Several options have been tested such as pharmacologically induced coronary artery dilation, blocking the venous return or developing a cardiac recirculation approach. Delivery methods based on cardiopulmonary bypass (CPB) with a closed-loop system can be used for cardiac gene therapy [75,76]. It might seem excessive to selectively prescribe CPB for gene delivery given the clearly invasive nature of such a procedure. Nevertheless, one should not forget that many of the CM/ heart failure patients might need invasive procedures due to their clinical condition. Gene therapy should also be evaluated in the context of combination therapies. CM/heart failure presents as a syndrome with multiple pathophysiological facets. Early treatment of some specific aspects like atrial fibrillation by gene therapy might be as efficient as conventional cardioversion. Targeting the autonomous nervous system through gene therapy should be evaluated with reference to current beta-blockers. Inotropism might be improved by means of additive gene therapy, for example. However, beyond the diversity of gene therapy targets, combination with more conventional drugs might be improved by reinforcing the

Gene Therapy for Cardiomyopathies http://dx.doi.org/10.5772/intechopen.80478

Gene therapy is emerging as a suitable alternative, with substantial progress in preclinical models of cardiovascular disorders. Despite the fact that none of the clinical trials, which investigated new treatments for CMs, has met their primary efficacy endpoints, subanalysis, however, has demonstrated potential efficacy. Inadequate gene delivery remains one of the underlying causes behind failures seen in clinical trials. Higher transduction efficiency is needed to achieve therapeutic effects. Use of block copolymers in gene delivery is a promising

CMs can serve as a disease model for several aspects when it comes to the development of gene therapy strategies in the context of cardiac diseases, since they also engulf inherited

area of research, in which new and important developments are expected.

target pathways.

**4. Conclusions**

diseases like acquired disorders.

The principal limitation of most non-clinical studies and some clinical trials was the inability to efficiently transfer genes to the cardiac ventricles. Although *in vivo* experiments using small animals may show efficient gene transfer, many fundamental differences exist between small animal and human hearts. Large animal studies are best suited for comprehensive evaluation at the preclinical stages of therapeutic development. It might seem obvious that delivery methods should meet all criteria of clinically relevant practices. Nevertheless, some preclinical methods seem to lack this realism.

#### **3.6. Administration strategies**

With regards to the first step to translate *in vivo* gene transfer into clinically relevant gene therapy and based at least partly on the use of naked DNA, physical methods like direct intra-myocardial injections have demonstrated feasibility, but also limited efficiency. Derived from these pioneering steps, several refinements have been introduced over time. In the context of rhythm control, one should look with interest to techniques like gene painting [72]. Gene painting refers basically to an innovative technique aimed at a very

**Figure 3.** Preclinical evaluation of intracoronary vector administration in large animals, for instance Beagle dogs. Similar amounts of cDNA were formulated differently. (A) Sample of a coronary contrast injection of the left main coronary artery in a dog heart highlighting the route of administration. (B) Mid-ventricular cross-section after AAV1 vectorization of a lacZ coding cDNA. (C) Mid-ventricular cross-section after polymer P85 vectorization of a lacZ coding cDNA. X-gal staining reveals lacZ gene expression (unpublished results).

local gene transfer by a topical administration. Proof-of-concept studies have shown the efficiency of this approach in atrial fibrillation. Strategies based on ultrasound-targeted microbubble destruction could be a promising method for gene delivery [73]. Microbubbles are small (<5 μm) gas-filled voids that are generally stabilized by phospholipids or synthetic polymers. The use of microbubbles as gene vectors is based on the paradigm that destruction of the DNA-loaded microbubbles by ultrasounds will result in local transduction and still spare non-target areas. Percutaneous antegrade coronary injections are among the least invasive cardiac selective gene delivery methods and are rather broadly available. Intracoronary delivery allows diffuse transduction throughout the myocardium, but as such it is a highly inefficient process. However, dense regional gene transfer (>80% of myocytes in the target territory) is possible. Pharmacological manipulations to induce vasodilation and maximize vascular permeability in a specific coronary perfusion territory can greatly improve transfection efficiency [74]. Given the high perfusion velocity and the submaximal extent of the vascular bed, one has to maximize the duration of vector exposure to the local vasculature while minimizing the systemic distribution. Several options have been tested such as pharmacologically induced coronary artery dilation, blocking the venous return or developing a cardiac recirculation approach. Delivery methods based on cardiopulmonary bypass (CPB) with a closed-loop system can be used for cardiac gene therapy [75,76]. It might seem excessive to selectively prescribe CPB for gene delivery given the clearly invasive nature of such a procedure. Nevertheless, one should not forget that many of the CM/ heart failure patients might need invasive procedures due to their clinical condition. Gene therapy should also be evaluated in the context of combination therapies. CM/heart failure presents as a syndrome with multiple pathophysiological facets. Early treatment of some specific aspects like atrial fibrillation by gene therapy might be as efficient as conventional cardioversion. Targeting the autonomous nervous system through gene therapy should be evaluated with reference to current beta-blockers. Inotropism might be improved by means of additive gene therapy, for example. However, beyond the diversity of gene therapy targets, combination with more conventional drugs might be improved by reinforcing the target pathways.
