**Author details**

Surprisingly, the use of the smallest impeller with the smaller BPS-i30 drive shows a comparable

processes. This circumstance is not foreseeable by the sole process engineering characterization, so that the additional use of a biological characterization approach becomes evident. The suitability of the presented developed bioreactor concept for microbial applications could be clearly demonstrated, even if it seems rather unusual in comparison to commercial systems due to its bottom drive without bearings. The complete characterization provides the possibility for an easier transfer to the industrial biopharmaceutical scale. Finally, the currently available bioengineering data of the new developed bioreactor indicate that the bioreactor operated with the BPS-i30 drive can also be used to grow animal cells. More detailed investigations are planned in the future.

*a* value for microbial

biomass concentration to bioreactors on a pilot scale despite a very low *kL*

**Abbreviations**

100 Biopharmaceuticals

**Nomenclature**

*CO*2

*CO*2

CCOS culture collection of Switzerland CFD computational fluid dynamics CHO Chinese hamster ovary cell line

CHO XM 111–10 SEAP secreting cell line

PBS phosphate-buffered saline

*μ* specific growth rate [h−1]

*cH* mixing number [−] *d* impeller diameter [m]

*D* vessel diameter [m] *DCW* dry cell weight [g·L−1] *DO* dissolved oxygen [%]

*H* vessel height [m]

*a* phase boundary interface [m−1]

SEAP secreted alkaline phosphatase of the placenta

<sup>∗</sup> maximum oxygen concentration [mmol·L−1]

present oxygen concentration [mmol·L−1]

*E. coli Escherichia coli* LB lysogenic broth

> Cedric Schirmer1 \*, Thomas Nussbaumer2 , Reto Schöb2 , Ralf Pörtner3 , Regine Eibl1 and Dieter Eibl1

\*Address all correspondence to: cedric.schirmer@zhaw.ch

1 School of Life Sciences and Facility Management, Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, Wädenswil, Switzerland

2 Levitronix GmbH, Zurich, Switzerland

3 Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Hamburg, Germany

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bp9801087


**Section 6**

**Regulation**

**Section 6**
