**Microscopic Dimensions**

**Chapter 2**

**Provisional chapter**

**Fluorescence Microscopic Spectroscopy for**

**Fluorescence Microscopic Spectroscopy for** 

Natalia Grigoryeva and Ludmila Chistyakova

Natalia Grigoryeva and Ludmila Chistyakova

Additional information is available at the end of the chapter

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.78044

biotechnology, harmful algal blooms

**Abstract**

**1. Introduction**

**Investigation and Monitoring of Biological Diversity**

**Investigation and Monitoring of Biological Diversity** 

In this chapter, a novel technique for investigation of natural and laboratory cyanobacterial cultures is presented. The technique is based on a strict relation between the intrinsic singlecell fluorescence emission spectra of cyanobacteria and the physiological state of the whole culture. It will be shown else that the single-cell fluorescence spectra for different species are steady enough to conduct a taxonomic analysis of cyanobacterial cultures based on a common statistical data evaluation among the parameters extracted from a set of such spectra. Several examples are given to illustrate the power and simplicity of a new technique, which can become a promising tool for automation of production in the cyanobacterial biotechnology, as well as give a valuable contribution to the development of innovative

**Keywords:** cyanobacteria, confocal laser scanning microscopy, single-cell fluorescence spectrum, biological diversity, physiological state, environmental monitoring,

Cyanobacteria have gained huge attention in recent years because of their potential application in biotechnology [1–5]. For example, cyanobacteria are considered as a rich source of biologically active compounds with antiviral, antibacterial, antifungal and anticancer activities. Several strains of cyanobacteria were found to accumulate polyhydroxyalkanoates, which can be used as a substitute for nonbiodegradable petrochemical-based plastics. Recent studies showed that oil-polluted sites are rich in cyanobacterial consortia capable of degrading

approaches in environmental monitoring of harmful algal blooms.

© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use,

distribution, and reproduction in any medium, provided the original work is properly cited.

DOI: 10.5772/intechopen.78044

**and Physiological State of Cyanobacterial Cultures**

**and Physiological State of Cyanobacterial Cultures**

#### **Fluorescence Microscopic Spectroscopy for Investigation and Monitoring of Biological Diversity and Physiological State of Cyanobacterial Cultures Fluorescence Microscopic Spectroscopy for Investigation and Monitoring of Biological Diversity and Physiological State of Cyanobacterial Cultures**

DOI: 10.5772/intechopen.78044

Natalia Grigoryeva and Ludmila Chistyakova Natalia Grigoryeva and Ludmila Chistyakova

Additional information is available at the end of the chapter Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.78044

#### **Abstract**

In this chapter, a novel technique for investigation of natural and laboratory cyanobacterial cultures is presented. The technique is based on a strict relation between the intrinsic singlecell fluorescence emission spectra of cyanobacteria and the physiological state of the whole culture. It will be shown else that the single-cell fluorescence spectra for different species are steady enough to conduct a taxonomic analysis of cyanobacterial cultures based on a common statistical data evaluation among the parameters extracted from a set of such spectra. Several examples are given to illustrate the power and simplicity of a new technique, which can become a promising tool for automation of production in the cyanobacterial biotechnology, as well as give a valuable contribution to the development of innovative approaches in environmental monitoring of harmful algal blooms.

**Keywords:** cyanobacteria, confocal laser scanning microscopy, single-cell fluorescence spectrum, biological diversity, physiological state, environmental monitoring, biotechnology, harmful algal blooms
