**3.2.1 Genetic suppression of hepatic GK**

A liver specific GK knock-out was obtained using the LoxP-Cre system (Postic, 1999). Transgenic mice showed mild hyperglycemia and hyperinsulinemia in basal conditions, without changes in hepatic glycogen, plasma non-esterified fatty acids, triglycerides or βhydroxybutyrate. In hyperglycaemic clamp studies, reduced hepatic glucose uptake and glycogen levels were observed in KO animals; however, results on lipid profile were not provided.
