**2. Preparation in theater**

It is mandatory for the surgeon in charge to check the documents: certification of brain death of the donor, the consent form for organ and tissue donation, signature of the hospital delegation, and the patient serology test. The team time-out should be carried out. The communication is confirmed between the surgeon and anesthetist for administration of medications at various stages. One dose of intravenous antibiotics is given intravenously, as well as 1 g of methylprednisolone prior to surgery. One dose of mannitol 20 g is given after completion of abdominal dissection. Heparin 25,000 IU is administered following cardiac team dissection prior to the cold perfusion.

If the pancreas is retrieved for organ transplantation, 100 ml of half-strength betadine (5%) is injected through the nasogastric tube that is then clamped off during procedure to retain betadine solution in the duodenum.

Preparation and drape: under sterile condition, the surgical area from the lower part of the chin to the proximal one-third of the thigh is prepared. The hairs are shaved. The dressing and central venous catheter or femoral catheter is reorganized to ensure the surgical field is clean and neat for preparation and drape.
