**3.3. Analysis of cell death by IncuCyteZOOM®**

Cells were seeded into flat-bottom 96-well microtiter plates (Sarstedt.com, Germany) (density: 17,000 cells per cm2 ). Semi-confluent cell layers were treated one day after seeding with DMSO (vehicle control) or Vac (Selleckchem.com, Germany; Stock: 76 mM in DMSO) in the presence of 10 μg/ml PI (Sigma.com, USA). Carvacrol was purchased from Sigma-Aldrich (Sigma. com, USA). Cell death was quantified as the red object count/image by the IncuCyteZOOM® software.
