**6.2. Probe placement**

In order to immobilize the biomolecules, probes or receptors are used in the nanogap. The placement of probes in the nanogap for immobilization of biomolecules may not be continuous throughout, and this may result in partially filled nanogaps [39]. For simulation, this may be considered in a similar manner as the steric hindrance except that the profiles shall not be continuous as in steric hindrance.
