**2. Natural antioxidants**

an imperative role, which can further supported by the supplementation of antioxidants in the diet. Generally, antioxidants can be divided into two major categories such as synthetic and natural. The main targeted site of these free radicals damage and defensive approach of antioxidants in the body is at the cellular level. Based up on this, these antioxidants can also be classified as enzymatic and nonenzymatic antioxidants. Enzymatic antioxidants primarily include glutathione peroxidase, catalase, and superoxide dismutase. There are also several other enzymes in the body that contribute to the total antioxidant capacity, which reflects in the serum [4]. Nonenzymatic antioxidants contain several subdivisions mainly vitamins such as A, E, C, and to lesser extent vitamin D, enzyme cofactors (Q10), peptides and some minerals (zinc and selenium). The major ingredients from the natural sources are polyphenolic compounds, which are reported to have significant antioxidant potential [5]. A detailed clas-

Natural antioxidants are primarily phenolics that may occur in all parts of plants [6], such as fruits, vegetables, nuts, seeds, leaves, roots, and barks. In the recent past, some toxicological studies regarding the use of synthetic antioxidants have shown their unwanted or adverse effects. These reports have urged the researchers to focus their study on exploring the natural sources with reasonable antioxidant potential [7]. Moreover, the availability and economy are significant concerns too in the context of using these natural antioxidants. The antioxidants from the nature can be categorized into the various subclassifications. However, two major categories are like antioxidants from commonly consumed or routine natural diets (e.g., vegetables, fruits, cereals, and beans) and secondly from plant or herb source that have fair antioxidant

sification and subclassification has been displayed in **Figure 1**.

4 Antioxidants in Foods and Its Applications

**Figure 1.** Classification and subclassification of antioxidants found in natural sources.

The nature is always a significant and rich source of countless ingredients that can be served as health-promoting agents. Many of these natural sources include routinely used fruits, vegetables, herbs, spices, and edible mushrooms that can be the part of routine diet. In addition to that, there is a huge list of medicinal plants reported to have extensive health-boosting potentials. One of the most beneficial effects from these natural sources is due to their potential antioxidant properties. Regarding the antioxidant capability, the researchers have focused their studies to explore the most potential sources along with their active ingredients. The researchers have added some marine sources such as algae and seagrass as well in the list of these natural sources. The recent studies have also explored the role of naturally occurring microbiome in the gut in the body antioxidant pool denoted as good bugs. These good bugs can also be used as supplement called as probiotics. Thus, generated literature is a big data bank for the researchers of the field and beneficial information for the general reader as well. There is a need to summarize this enormous data bank based upon the identification of most potential sources, which will make the literature feasible for the professional and nonprofessional readers at the same time. This chapter will elaborate the various natural sources of antioxidants that hopefully help to prioritize the beneficial amendments in dietary composition.

**No English name Antioxidant contents Concentration** 

myricetin, kaempferol, and luteolin), betalains, taurine, total carotenoids and total phenolics

2. Guava β-Carotene, lycopene, vitamin C, ellagic acid, anthocyanin

7. Apple Proanthocyanidins, flavonoids (kaempferol,

hydroxycinnamates

8. Plum Proanthocyanidins, flavonoids (kaempferol,

9. Guava β-Carotene, lycopene, vitamin C, ellagic acid, anthocyanin

10. Beet root Betalains, vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

11. Pea Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

12. Carrot Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

13. White cabbage Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

14. Tomato Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

15. White onion Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

16. Cauliflower Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

17. Spinach Vitamins C and E, carotenoids, flavonoids, and thiol (SH) compounds

**Table 1.** Some important fruits having antioxidant constituents.

glycosides (vomifoliol derivatives)

glycosides (vomifoliol derivatives)

Flavanols, flavonols, dihydrochalcones, and

3. Pears Ascorbic acid, flavonoids (quercetin, isorhamnetin,

1. Beet root Betalains [146]

4. Pomegranate Vitamin C and polyphenols 1245 (mmol TE/g) [149] 5. Papaya Quercetin and β-sitosterol 300 (mmol TE/g) [150] 6. Water melon Lycopene, β-carotene, vitamin C 100 [151]

> quercetin, and naringenin derivatives); phenolic acids (protocatechuic, caffeoylquinic, and hydroxycinnamic acid derivatives); hydroxychalcones (phloretin and 3-hydroxyphloretin derivatives); and isoprenoid

> quercetin, and naringenin derivatives); phenolic acids (protocatechuic, caffeoylquinic, and hydroxycinnamic acid derivatives); hydroxychalcones (phloretin and 3-hydroxyphloretin derivatives); and isoprenoid

**(ORAC value)**

16.78 a ± 0.25 (mmol TE/g) 1508 ± 44 (μmol/100 g)

14.55 c ± 0.21 (mmol TE/g) 94.8 (mmol TE/g)

4100 (dry extract) 115 (μmol TE/g)

19 (μmol TE/g) [155]

60 (μmol TE/g) [155]

61 (μmol TE/g) [155]

67 (μmol TE/g) [155]

85 (μmol TE/g) [155]

102 (μmol TE/g) [155]

152 (μmol TE/g) [155]

135 (mmol TE/g) [148]

http://dx.doi.org/10.5772/intechopen.75961

**References**

7

[147]

Antioxidants from Natural Sources

[152] [153]

[152] [154]

[146] [155]

Fruits and vegetables are highly recommended dietary contents, widely known for their health-promoting effects and nutritious values. They got an essential place as conventional foods in the history because of their excessive amount of minerals, specifically electrolytes; vitamins, specifically vitamins C and E; while various current studies are revealing their phytochemical contents, having antioxidant capabilities [13]. These antioxidants scavenge the oxidants or free radicals produced as a result of several degenerative and disease processes such as diabetes, cancers, and cardiovascular disorders. So, regular consumption of fruits and vegetables may reduce the risk of mortality associated with these diseases [14]. Most of the natural antioxidants convert the lipid radicals into more stable products by breaking the chain. Antioxidants obtained from vegetables and fruits are mostly of phenolic structure, which may include vitamins, minerals, and polyphenols [15]. Antioxidant minerals, such as iron, zinc, selenium, copper, and manganese, act as cofactor of many antioxidant enzymes, absence of which may certainly disturb the activity of their enzymatic scavenging activity [16]. The antioxidant capability of different fruits is being presented in **Table 1**.

#### **2.1. Types of antioxidants from fruits and vegetables**

Polyphenols, present in fruits and vegetables, is a group of several low- and high-molecularweight compounds having antioxidant properties that prevent lipid oxidation [17]. Most of them are conjugates of mono and polysaccharides connected with one or more groups of phenol rings or may also present as functional derivatives such as esters and methyl esters [18]. This major class of natural antioxidants can be obtained from teas, particularly green and red teas, as well as fruits such as grapes [19]. However, polyphenols from teas have more significant than in fruits because of their bioavailability in blood. Approximately 15–20% polyphenols are absorbed in human blood from their consumed amount. This absorption is enhanced when there are no sugar molecules attached with them. So, teas have more absorption of polyphenols than in fruits because of high sugar content in fruits [20].

Flavonoids, another important antioxidant content, is a subclass of polyphenols present abundantly in most of the foods, such as potatoes, wheat, tomatoes, red berries, peaches,


**Table 1.** Some important fruits having antioxidant constituents.

herbs, spices, and edible mushrooms that can be the part of routine diet. In addition to that, there is a huge list of medicinal plants reported to have extensive health-boosting potentials. One of the most beneficial effects from these natural sources is due to their potential antioxidant properties. Regarding the antioxidant capability, the researchers have focused their studies to explore the most potential sources along with their active ingredients. The researchers have added some marine sources such as algae and seagrass as well in the list of these natural sources. The recent studies have also explored the role of naturally occurring microbiome in the gut in the body antioxidant pool denoted as good bugs. These good bugs can also be used as supplement called as probiotics. Thus, generated literature is a big data bank for the researchers of the field and beneficial information for the general reader as well. There is a need to summarize this enormous data bank based upon the identification of most potential sources, which will make the literature feasible for the professional and nonprofessional readers at the same time. This chapter will elaborate the various natural sources of antioxidants that hopefully help to prioritize the beneficial

Fruits and vegetables are highly recommended dietary contents, widely known for their health-promoting effects and nutritious values. They got an essential place as conventional foods in the history because of their excessive amount of minerals, specifically electrolytes; vitamins, specifically vitamins C and E; while various current studies are revealing their phytochemical contents, having antioxidant capabilities [13]. These antioxidants scavenge the oxidants or free radicals produced as a result of several degenerative and disease processes such as diabetes, cancers, and cardiovascular disorders. So, regular consumption of fruits and vegetables may reduce the risk of mortality associated with these diseases [14]. Most of the natural antioxidants convert the lipid radicals into more stable products by breaking the chain. Antioxidants obtained from vegetables and fruits are mostly of phenolic structure, which may include vitamins, minerals, and polyphenols [15]. Antioxidant minerals, such as iron, zinc, selenium, copper, and manganese, act as cofactor of many antioxidant enzymes, absence of which may certainly disturb the activity of their enzymatic scavenging activity

[16]. The antioxidant capability of different fruits is being presented in **Table 1**.

polyphenols than in fruits because of high sugar content in fruits [20].

Polyphenols, present in fruits and vegetables, is a group of several low- and high-molecularweight compounds having antioxidant properties that prevent lipid oxidation [17]. Most of them are conjugates of mono and polysaccharides connected with one or more groups of phenol rings or may also present as functional derivatives such as esters and methyl esters [18]. This major class of natural antioxidants can be obtained from teas, particularly green and red teas, as well as fruits such as grapes [19]. However, polyphenols from teas have more significant than in fruits because of their bioavailability in blood. Approximately 15–20% polyphenols are absorbed in human blood from their consumed amount. This absorption is enhanced when there are no sugar molecules attached with them. So, teas have more absorption of

Flavonoids, another important antioxidant content, is a subclass of polyphenols present abundantly in most of the foods, such as potatoes, wheat, tomatoes, red berries, peaches,

**2.1. Types of antioxidants from fruits and vegetables**

amendments in dietary composition.

6 Antioxidants in Foods and Its Applications

and almonds [21]. Anthocyanin is a subcategory of flavonoids (**Figure 1**) which is present in berries and red wine. It is a potent antioxidant with decreased bioavailability as compared to other flavonoids [19]. Polyphenols show their antioxidant properties by preventing the oxidation of low-density lipoproteins (LDL), thus preventing plaque formation [22]. Some types of polyphenols have also been found to inhibit the oxidation of some important enzymes and thus preserve their proper functioning [23]. Carotenoids are another major class of phytochemicals antioxidants from fruits and vegetables after polyphenols. They mostly found in vegetables, such as potatoes, carrots, papayas, and apricots [21].

wastes of wine-making industry include degradable solids such as skin, stem, and seeds, which contain many antioxidants that have shown to prevent many degenerative processes and possess health-promoting effects [37]. Pujol et al. [38] reported that the coffee wastes from the coffee industry contains approximately 6% polyphenols and about 4% tannins. Antioxidant ingredients from various sources of fruit waste have been presented in **Table 2**.

**No Fruit Residue Antioxidant Reference**

3. Water melon Peel, rinds Citrulline, lycopene, flavonoids, and phenols [160, 161] 4. Cucumber Peel Flavonoids and phenols [33] 5. Potato Peel Chlorogenic acid, caffeic acid, ferulic acid, and

phenols

procyanidins

condensed tannins

Phenols and flavonoids [156, 157]

Polyphenols, tannins, and gallic acids [164, 38]

quercetin glycosides, chlorogenic acid, hydroxycinnamates, phloretin glycosides, and

Coumaric acid, caffeic acid, ferulic acid, chlorogenic acid, cinnamic acid, neochlorogenic acid, p-hydroxybenzoic acid, protocatechuic acid, vanillic acid, gallic acid, proanthocyanidins, quercetin 3-o-gluuronide,

Catechin, cyanidin 3-glucoside, galangin, gallic acid, homogentisic acid, and kaempferol

chlorogenic acid, ferulic acid, and phenols

Carotenoids [172]

Gallic acid, cyanidin-3,5-diglucoside, cyanidin-3-diglucoside, and delphinidin-3,5-diglucoside

quercetin, and resveratrol

phellandrene, caryophyllene

[158, 159]

Antioxidants from Natural Sources

9

http://dx.doi.org/10.5772/intechopen.75961

[162, 163]

[165]

[166, 167]

[168]

[169, 170]

[33, 160]

[160, 162, 163]

(green) fruit and peel

ground and residue

7. Apple Peel Epicatechin, catechins, anthocyanins,

seeds

seeds

pericarp

12. Cucumber Peel Phenols, flavonoids, pheophytin,

pomace

13. Potato Peel Gallic acid, caffeic acid, vanillic acid,

11. Carrot Peel Phenols, β-carotene [171]

2. Mango Peel, kernel Gallic acid, ellagic acid, gallates, gallotannins,

1. Banana Unripe

6. Coffee Coffee

8. Grapes Skin and

9. Guava Skin and

10. Pomegranate Peel and

14. Tomato Skin and

**Table 2.** Antioxidants from some fruits and vegetable wastes.

**Vegetables**

Among the vitamins obtained from fruits and vegetables, acting as antioxidants, vitamin C, also known as ascorbic acid, is a very potent water-soluble antioxidant commonly found in citrus fruits and vegetables such as oranges, lemons [24], and tomatoes [21]. It is recommended that the fruits and vegetables containing vitamin C should be taken in small divided doses instead of having a large dose simultaneously because vitamin C shows less absorption when given in large quantities [25].

Another vitamin with antioxidant properties is vitamin E, which is related to tocopherol family of antioxidant. It is a fat-soluble, nonpolar vitamin found naturally in lipid-rich fruits and vegetables, such as olives, sunflower, and nuts [21]. Vitamin E shows higher bioavailability than vitamin C, which is perhaps due to its fat solubility and can be further enhanced when taken with fatty foods [25].

## **2.2. Antioxidants from fruits and vegetables wastes**

Fruits and vegetable waste material is produced during their cultivation, industrial management, processing, preservation, and distribution [26]. In the past few decades, researchers have been struggling to device the methods to recycle these wastes to get therapeutic benefits [27]. The vegetables and fruit waste material includes peelings, trimming, shells, seeds, stems, and pulp residues that remained after extraction of juices and starch or sugar preparation. This waste constitutes about 25–30% [28]. According to a research, greater amounts of phenols and ascorbic acids have been reported in these waste scalps than their pulp [29] and in unripe form than their ripened form [30]. Most of the fruit peels contain 2–27 times greater amount of antioxidants than their pulp [31]. According to Someya et al., banana pulp possesses 232 mg/100 g of phenolic components, and this amount is just 25% of the amount present in banana peels [32]. *Cucumis sativus* (Cucumber) peel has been reported as a good source of flavonoids, which is considered as a potential antioxidant [33]. Many bioactive substances may be recovered from theses wastes to prepare foods in food processing and therapeutic preparations [34]. A significant amount of bioactive phytochemicals, having strong antioxidant properties, can be obtained from the tomato wastes, which include carotenes, tocopherols, terpenes, sterols, and polyphenols. These natural antioxidants obtained from food waste may be used to formulate functional foods or can be used as food additives [35].

A lot of antioxidants, such as carotenoids, phenolic compounds, vitamin C, and dietary fibers, are found in mango peel. These compounds have reported activity against many degenerative diseases, such as Alzheimer's disease, cataracts, cancer, and Parkinson's disease [36]. The wastes of wine-making industry include degradable solids such as skin, stem, and seeds, which contain many antioxidants that have shown to prevent many degenerative processes and possess health-promoting effects [37]. Pujol et al. [38] reported that the coffee wastes from the coffee industry contains approximately 6% polyphenols and about 4% tannins. Antioxidant ingredients from various sources of fruit waste have been presented in **Table 2**.

and almonds [21]. Anthocyanin is a subcategory of flavonoids (**Figure 1**) which is present in berries and red wine. It is a potent antioxidant with decreased bioavailability as compared to other flavonoids [19]. Polyphenols show their antioxidant properties by preventing the oxidation of low-density lipoproteins (LDL), thus preventing plaque formation [22]. Some types of polyphenols have also been found to inhibit the oxidation of some important enzymes and thus preserve their proper functioning [23]. Carotenoids are another major class of phytochemicals antioxidants from fruits and vegetables after polyphenols. They mostly found in

Among the vitamins obtained from fruits and vegetables, acting as antioxidants, vitamin C, also known as ascorbic acid, is a very potent water-soluble antioxidant commonly found in citrus fruits and vegetables such as oranges, lemons [24], and tomatoes [21]. It is recommended that the fruits and vegetables containing vitamin C should be taken in small divided doses instead of having a large dose simultaneously because vitamin C shows less absorption

Another vitamin with antioxidant properties is vitamin E, which is related to tocopherol family of antioxidant. It is a fat-soluble, nonpolar vitamin found naturally in lipid-rich fruits and vegetables, such as olives, sunflower, and nuts [21]. Vitamin E shows higher bioavailability than vitamin C, which is perhaps due to its fat solubility and can be further enhanced when

Fruits and vegetable waste material is produced during their cultivation, industrial management, processing, preservation, and distribution [26]. In the past few decades, researchers have been struggling to device the methods to recycle these wastes to get therapeutic benefits [27]. The vegetables and fruit waste material includes peelings, trimming, shells, seeds, stems, and pulp residues that remained after extraction of juices and starch or sugar preparation. This waste constitutes about 25–30% [28]. According to a research, greater amounts of phenols and ascorbic acids have been reported in these waste scalps than their pulp [29] and in unripe form than their ripened form [30]. Most of the fruit peels contain 2–27 times greater amount of antioxidants than their pulp [31]. According to Someya et al., banana pulp possesses 232 mg/100 g of phenolic components, and this amount is just 25% of the amount present in banana peels [32]. *Cucumis sativus* (Cucumber) peel has been reported as a good source of flavonoids, which is considered as a potential antioxidant [33]. Many bioactive substances may be recovered from theses wastes to prepare foods in food processing and therapeutic preparations [34]. A significant amount of bioactive phytochemicals, having strong antioxidant properties, can be obtained from the tomato wastes, which include carotenes, tocopherols, terpenes, sterols, and polyphenols. These natural antioxidants obtained from food waste may be used to formulate functional foods or can be

A lot of antioxidants, such as carotenoids, phenolic compounds, vitamin C, and dietary fibers, are found in mango peel. These compounds have reported activity against many degenerative diseases, such as Alzheimer's disease, cataracts, cancer, and Parkinson's disease [36]. The

vegetables, such as potatoes, carrots, papayas, and apricots [21].

**2.2. Antioxidants from fruits and vegetables wastes**

when given in large quantities [25].

8 Antioxidants in Foods and Its Applications

taken with fatty foods [25].

used as food additives [35].


**Table 2.** Antioxidants from some fruits and vegetable wastes.

#### **2.3. Mushrooms as antioxidant**

In the nutrition world, mushrooms are delegated vegetables; however, they are not actually plants. They have a place with the kingdom of fungi. In spite of the fact that they are not vegetables, mushrooms give imperative supplements. Mushrooms are considered as healthfully utilitarian sustenance and also source of valuable medicines [39, 40]. Numerous consumable mushrooms (for the most part Basidiomycetes) are great wellsprings of important nutritive components including carbohydrates, for example, β-glucans; lipids; B-vitamins, such as niacin, flavin, and pyridoxine; phenolics, like tocopherols; organic acids, for example, malate ascorbate, fumarate, and shikimate; monoterpenoid and diterpenoid; proteins, for example, hydrophobins; and trace components, for example, selenium [41–43]. These components are established as to be responsible for immunomodulatory, antimicrobial, antitumor, antihypertensive, hepatoprotective, and antioxidant activities of mushrooms [44, 45].

boreal, and tropical forests [65]. It grows on living trees and on dead and rotting sustenance material [66]. Polysaccharides obtained from wild sporophores and refined products of *A. mellea* have scavenging properties [67]. Two polysaccharides, AkPS1V-1 and AkPS1V-2, from the alkaline extract of the mushroom have been isolated and reported for their antioxidant activity [68]. Moreover, their ascorbic acid and phenolic components have been reported for their scavenging impact on superoxide anions [69]. Overall, it is reported to have a fair anti-

Antioxidants from Natural Sources

11

http://dx.doi.org/10.5772/intechopen.75961

*A. auricula* is an edible mushroom, found worldwide, belongs to the family Auriculaceae, commonly known as tree-tea or wood-ear. The mushroom contains high quantity of carbohydrates including polysaccharides, proteins, minerals, and phenolic substances [69, 70]. Polysaccharides of the mushroom have antioxidant activity [71] by the inhibition of lipid peroxidation and have effective hydroxyl radical scavenging activity [72]. In the case of lipid peroxidation, IC50 values of ethanolic, crude, and boiled extracts of *A. auricula* are 398, 310, and 572 μg/ml, respectively, and in the case of hydroxyl radical scavenging activity, 373, 403, and 510 μg/ml, respectively [72]. Khaskheli et al. isolated two polysaccharides from fruiting body of *A. auricula* and evaluated potential antioxidant activity of these polysaccharides [71]. Among its various extracts, the boiled extract, which is also convenient, proves to be more

*B. edulis* belongs to the Boletus species of mushroom widely distributed in the holarctic across Asia, Europe, and North America, commonly known as porcino and penny bun [73]. Other edible mushrooms of boletus species including *B. aereus, B. reticulate*, and *B. edulis* have good antioxidant properties. Total phenol contents of *B. edulis* is higher that shows the scavenging activity [74]. *B. auranticus* (EC50 0.016 mg/ml) exhibits higher total phenol contents as well as hydroxyl radical-scavenging activity than *B. edulis*. However, reducing power of *B. edulis* is higher. *B. edulis* extricate averts lipid peroxidation [75]. Antioxidant activity of *B. edulis* is attributed to its polysaccharides found in the fruiting bodies that are reported to have chelating action and inhibitory impacts on superoxide radical and hydroxyl radical [67]. Vieira et al. [76] investigate the *B. edulis* antioxidant activity with the combination of another edible mushroom *Marasmius oreades*. They investigated different parameter to observe the antioxidant capacity of the both mushroom species mixture, and they observed the good antioxidant activity in synergism. These results show that on various occasions, the addition of

more than one mushroom as a combination gives more effective results than alone.

*Ganoderma lucidum* is also commonly known as Lingzhi, a basidiomycete fungus, native to China and grows in mountain woods with humid and dim-light conditions, in the rotten bark or root of tree. The mushroom is well known as medicinal mushroom and has been prescribed to prevent and treat different diseases [77]. *G. lucidum* contains polysaccharides, sterols, triterpenoids,

oxidant capacity to be used as food oxidation-reducing substance.

*2.3.3. Auricularia auricula*

effective antioxidant.

*2.3.4. Boletus edulis*

*2.3.5. Ganoderma lucidum*

The amount of mushrooms on Earth is assessed at 140,000 yet might be just 10% (approximately 15,000 species) are known [43, 46]. Out of approximately 15,000 known species, 2,000 are found safe for human utilization, and around 650 of these contain therapeutic properties [47]. There are a number of mushrooms including *Agaricus bisporus* [48] *Lentinus edodes* [49], *Armillaria mellea* [50], *Auricularia auricula* [51], *Boletus edulis* [52], *Ganoderma applanatum* [53]*, Grifola frondosa* [54], *Hypsizigus marmoreus* [55], *Pleurotus* sp. [56], *Schizophyllum commune* [57], *Termitomyces* sp. [58], and *Tricholoma* sp. [59] that possess antioxidant properties. The antioxidant properties of mushrooms are mainly due to their phenolic compound [52, 48]. Phenolic acid is the chief phenolic component present in the mushrooms. There are an assortment of phenolic compounds recognized in wild mushrooms, including cinnamic acid, protocatechuic, *p*-hydroxybenzoic, *p*-coumaric acids, gallic acid, vanillin, rutin, and quercetin [60]. Polysaccharides are one of the major components in the mushroom. In recent studies, it has been revealed that they contain the antioxidant property [61]. Scavenging properties of polysaccharides are impacted by chemical properties such as atomic weight, level of branching, monosaccharide types, and proportion of monosaccharides, intermolecular relationship of polysaccharides, and alteration of polysaccharides. Among the monosaccharides, rhamnose is the most critical determinant factor related to scavenging properties of mushroom [62].

#### *2.3.1. Agaricus bisporus*

*A. bisporus,* also known as white button mushroom native to grasslands in Europe and North America, cultivated in more than 70 countries [63], possesses polysaccharides and antioxidants including vitamin C, D, B12; folates; and polyphenols [48]. The phenolic composition of methanolic extract from *A. bisporus* is investigated by HPLC. Polysaccharides and phenolic compounds such as gallic acid, rutin, caffeic acid, and catechin found in the mushroom are responsible for its scavenging activity [64]. Mushrooms being routinely used as dietary ingredient made them a significant natural antioxidant source.

#### *2.3.2. Armillaria mellea*

*A. mellea* is a culinary-medicinal honey mushroom, used as an admired element in the traditional Chinese medicine. The mushroom is pathogenic and is found worldwide in temperate, boreal, and tropical forests [65]. It grows on living trees and on dead and rotting sustenance material [66]. Polysaccharides obtained from wild sporophores and refined products of *A. mellea* have scavenging properties [67]. Two polysaccharides, AkPS1V-1 and AkPS1V-2, from the alkaline extract of the mushroom have been isolated and reported for their antioxidant activity [68]. Moreover, their ascorbic acid and phenolic components have been reported for their scavenging impact on superoxide anions [69]. Overall, it is reported to have a fair antioxidant capacity to be used as food oxidation-reducing substance.

#### *2.3.3. Auricularia auricula*

**2.3. Mushrooms as antioxidant**

10 Antioxidants in Foods and Its Applications

*2.3.1. Agaricus bisporus*

*2.3.2. Armillaria mellea*

In the nutrition world, mushrooms are delegated vegetables; however, they are not actually plants. They have a place with the kingdom of fungi. In spite of the fact that they are not vegetables, mushrooms give imperative supplements. Mushrooms are considered as healthfully utilitarian sustenance and also source of valuable medicines [39, 40]. Numerous consumable mushrooms (for the most part Basidiomycetes) are great wellsprings of important nutritive components including carbohydrates, for example, β-glucans; lipids; B-vitamins, such as niacin, flavin, and pyridoxine; phenolics, like tocopherols; organic acids, for example, malate ascorbate, fumarate, and shikimate; monoterpenoid and diterpenoid; proteins, for example, hydrophobins; and trace components, for example, selenium [41–43]. These components are established as to be responsible for immunomodulatory, antimicrobial, antitumor, antihyper-

The amount of mushrooms on Earth is assessed at 140,000 yet might be just 10% (approximately 15,000 species) are known [43, 46]. Out of approximately 15,000 known species, 2,000 are found safe for human utilization, and around 650 of these contain therapeutic properties [47]. There are a number of mushrooms including *Agaricus bisporus* [48] *Lentinus edodes* [49], *Armillaria mellea* [50], *Auricularia auricula* [51], *Boletus edulis* [52], *Ganoderma applanatum* [53]*, Grifola frondosa* [54], *Hypsizigus marmoreus* [55], *Pleurotus* sp. [56], *Schizophyllum commune* [57], *Termitomyces* sp. [58], and *Tricholoma* sp. [59] that possess antioxidant properties. The antioxidant properties of mushrooms are mainly due to their phenolic compound [52, 48]. Phenolic acid is the chief phenolic component present in the mushrooms. There are an assortment of phenolic compounds recognized in wild mushrooms, including cinnamic acid, protocatechuic, *p*-hydroxybenzoic, *p*-coumaric acids, gallic acid, vanillin, rutin, and quercetin [60]. Polysaccharides are one of the major components in the mushroom. In recent studies, it has been revealed that they contain the antioxidant property [61]. Scavenging properties of polysaccharides are impacted by chemical properties such as atomic weight, level of branching, monosaccharide types, and proportion of monosaccharides, intermolecular relationship of polysaccharides, and alteration of polysaccharides. Among the monosaccharides, rhamnose is the most critical determinant factor related to scavenging properties of mushroom [62].

*A. bisporus,* also known as white button mushroom native to grasslands in Europe and North America, cultivated in more than 70 countries [63], possesses polysaccharides and antioxidants including vitamin C, D, B12; folates; and polyphenols [48]. The phenolic composition of methanolic extract from *A. bisporus* is investigated by HPLC. Polysaccharides and phenolic compounds such as gallic acid, rutin, caffeic acid, and catechin found in the mushroom are responsible for its scavenging activity [64]. Mushrooms being routinely used as dietary ingre-

*A. mellea* is a culinary-medicinal honey mushroom, used as an admired element in the traditional Chinese medicine. The mushroom is pathogenic and is found worldwide in temperate,

dient made them a significant natural antioxidant source.

tensive, hepatoprotective, and antioxidant activities of mushrooms [44, 45].

*A. auricula* is an edible mushroom, found worldwide, belongs to the family Auriculaceae, commonly known as tree-tea or wood-ear. The mushroom contains high quantity of carbohydrates including polysaccharides, proteins, minerals, and phenolic substances [69, 70]. Polysaccharides of the mushroom have antioxidant activity [71] by the inhibition of lipid peroxidation and have effective hydroxyl radical scavenging activity [72]. In the case of lipid peroxidation, IC50 values of ethanolic, crude, and boiled extracts of *A. auricula* are 398, 310, and 572 μg/ml, respectively, and in the case of hydroxyl radical scavenging activity, 373, 403, and 510 μg/ml, respectively [72]. Khaskheli et al. isolated two polysaccharides from fruiting body of *A. auricula* and evaluated potential antioxidant activity of these polysaccharides [71]. Among its various extracts, the boiled extract, which is also convenient, proves to be more effective antioxidant.

#### *2.3.4. Boletus edulis*

*B. edulis* belongs to the Boletus species of mushroom widely distributed in the holarctic across Asia, Europe, and North America, commonly known as porcino and penny bun [73]. Other edible mushrooms of boletus species including *B. aereus, B. reticulate*, and *B. edulis* have good antioxidant properties. Total phenol contents of *B. edulis* is higher that shows the scavenging activity [74]. *B. auranticus* (EC50 0.016 mg/ml) exhibits higher total phenol contents as well as hydroxyl radical-scavenging activity than *B. edulis*. However, reducing power of *B. edulis* is higher. *B. edulis* extricate averts lipid peroxidation [75]. Antioxidant activity of *B. edulis* is attributed to its polysaccharides found in the fruiting bodies that are reported to have chelating action and inhibitory impacts on superoxide radical and hydroxyl radical [67]. Vieira et al. [76] investigate the *B. edulis* antioxidant activity with the combination of another edible mushroom *Marasmius oreades*. They investigated different parameter to observe the antioxidant capacity of the both mushroom species mixture, and they observed the good antioxidant activity in synergism. These results show that on various occasions, the addition of more than one mushroom as a combination gives more effective results than alone.

#### *2.3.5. Ganoderma lucidum*

*Ganoderma lucidum* is also commonly known as Lingzhi, a basidiomycete fungus, native to China and grows in mountain woods with humid and dim-light conditions, in the rotten bark or root of tree. The mushroom is well known as medicinal mushroom and has been prescribed to prevent and treat different diseases [77]. *G. lucidum* contains polysaccharides, sterols, triterpenoids, nucleosides, and alkaloids [78]. *G. lucidum* is called as marvelous mushroom of immortality because it shows that the consumption of the mushroom can prolong life [77]. Shi et al. [79] separated four polysaccharides from *G. lucidum* and investigate their antioxidant property. They demonstrated that these four polysaccharides have scavenging activities in a concentrationdependent manner [79]. *G. applanatum* is known as shelf fungus and also belongs to *Ganoderma* species of the mushroom. *G. applanatum* exhibits the higher antioxidant property over *G. lucidum* and other two edible mushrooms including *L. edodes* and *Trametes versicolor* [80].

*2.3.9. Pleurotus ostreatus*

*2.3.10. Schizophyllum commune*

ponents [97].

*2.4.1. Allium sativum*

*2.4.2. Capsicum annuum*

enoids [103].

*2.4.3. Curcuma longa*

the strong reducing power than alternate concentrates.

**2.4. Medicinal plants and spices having antioxidants**

plant gets older, more the antioxidant potential it will gain.

*Pleurotus ostreatus* is the third most cultivated mushroom worldwide after *A. bisporus* [94]. Its mycelia as well as fruiting bodies have well-known therapeutic effects due to its various biologically active compounds including phenols, flavonoids, and carotenoids [95] having strong antioxidant activity [96]. The methanol extract demonstrated the most grounded β-carotenelinoleic acid restraint when contrasted with alternate extracts. On the other hand, acetone has

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*S. commune* is a standout among the usually discovered fungus and can be separated from all landmasses, aside from Antarctica. *S. commune* has been accounted for to be a pathogen of people and trees; however, it principally receives a saprobic way of life by causing white rot. The antioxidant activity of *S. commune* is due to polysaccharides and polyphenols com-

*A. sativum* commonly known as garlic is a species belongs to family Alliaceae commonly cultivated in India [98]. It is a perpetual herb with a tall, erect blooming stem that grows up to 3 feet. Garlic has been utilized all through history for both culinary and therapeutic purposes. *A. sativum* is an adaptable herb that contains various trace elements, vitamins, and minerals. The total phenolic compound of the garlic has the antioxidant activity [99]. As an antioxidant, garlic has the strongest DPPH-scavenging ability [100]. Aged garlic extract has significantly eminent total phenolic substance than raw garlic extract [101]. It has been noticed that as the

*Capsicum annuum* (red pepper) is native to southern North America and northern South America and was introduced in Asia in sixteenth century from South America [102]. It contains a wide cluster of phytochemicals with their radical-scavenging properties [103]. The spice contains carotenoids, flavonoids, tocopherols, free sugars, capsaicinoids, L-ascorbic acid, and organic acids [104]. At the ripe stage, hot-dried peppers have a high bioactive substances that show huge free radical-scavenging properties such as polyphenols and carot-

*Curcuma longa* is a well-known spice that has a place in the Zingiberaceae family and is a lasting herb that measures up to 1 m high with a short stem. It is circulated all through tropical and subtropical locales of the world, being generally developed in Asiatic nations [105], primarily in India and China. In Pakistan and India, it is prevalently known as Haldi. As a powder, called turmeric, it has been in continual use as a flavor enhancer in both veggies lover

#### *2.3.6. Grifola frondosa*

*G. frondosa*, also known as Maitake, is a culinary as well as medicinal mushroom native to China, North America, and northeastern part of Japan but cultivated worldwide in several countries because of its useful effects [81]. The mushroom is progressively being perceived as a powerful wellspring of polysaccharide with sensational well-being and advanced potential. Total phenols, ascorbic acid, α-tocopherol, and flavonoids are the major scavenging agents present in the different *G. frondosa* extricates [82]. *G. frondosa* polysaccharides have critical inhibitory impacts on hydroxyl radical, superoxide radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical [83]. Their ferrous particles chelating activity is also strong [84]. Fan et al. [85] extracted five polysaccharides from the natural product group of *G. frondosa* by various separating techniques. Hot alkali extract of *G. frondosa* has the better antioxidant activity as compared to partially purified polysaccharides. It has the lower EC50 values of DPPH scavenging ability [86]. The study results enforce the variation of their antioxidant ingredients due to the seasonal and geographic displacements.

#### *2.3.7. Hypsizigus marmoreus*

*H. marmoreus* is an edible mushroom commonly found in East Asia including Korea, China, and Japan, widely known due to its antioxidant activity [87]. Phenols [88] and polysaccharides are the major bioactive components present in *H. marmoreus* that show the antioxidant activity by scavenging reactive oxygen species and strengthening its reducing power [55]. Liu et al. investigated the intracellular polysaccharides of *H. marmoreus* and revealed that these polysaccharides can be utilized as an antioxidant agent that improves adaptive immune reactions [89].

#### *2.3.8. Lentinus edodes*

*L. edodes* also known as shitake mushroom is the second most well-known consumable mushroom in the worldwide market and is usually cultivated in Indonesia, Taiwan, China, and Japan [90]. The mushroom is credited to its wholesome incentive as well as to conceivable potential for therapeutic applications [91]. *L. edodes* contains some important polysaccharides that have therapeutic activity. The mushroom is also a good source of vitamins, particularly vitamin B, including B1, B2, B5, B12, and provitamin D2. *L. edodes* extract has potent antioxidant effect due to the presence of bioactive compounds ergothioneine [92]. *L. edodes* has the ability to increase the total antioxidant capacity and reduce the total oxidative stress [91]. UV-C radiation can improve scavenging capacity of *L. edodes* [93]. Chen et al. [83] separated three types of polysaccharide from fruiting bodies of *L. edodes*, and they demonstrated the polysaccharides as potent antioxidant agents that can produce healthy immune response. The treatment of mushroom crude powder or its extract may also helpful to enhance its antioxidant capability.

#### *2.3.9. Pleurotus ostreatus*

nucleosides, and alkaloids [78]. *G. lucidum* is called as marvelous mushroom of immortality because it shows that the consumption of the mushroom can prolong life [77]. Shi et al. [79] separated four polysaccharides from *G. lucidum* and investigate their antioxidant property. They demonstrated that these four polysaccharides have scavenging activities in a concentrationdependent manner [79]. *G. applanatum* is known as shelf fungus and also belongs to *Ganoderma* species of the mushroom. *G. applanatum* exhibits the higher antioxidant property over *G. lucidum*

*G. frondosa*, also known as Maitake, is a culinary as well as medicinal mushroom native to China, North America, and northeastern part of Japan but cultivated worldwide in several countries because of its useful effects [81]. The mushroom is progressively being perceived as a powerful wellspring of polysaccharide with sensational well-being and advanced potential. Total phenols, ascorbic acid, α-tocopherol, and flavonoids are the major scavenging agents present in the different *G. frondosa* extricates [82]. *G. frondosa* polysaccharides have critical inhibitory impacts on hydroxyl radical, superoxide radical, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical [83]. Their ferrous particles chelating activity is also strong [84]. Fan et al. [85] extracted five polysaccharides from the natural product group of *G. frondosa* by various separating techniques. Hot alkali extract of *G. frondosa* has the better antioxidant activity as compared to partially purified polysaccharides. It has the lower EC50 values of DPPH scavenging ability [86]. The study results enforce the variation of their antioxidant ingredients due to the

*H. marmoreus* is an edible mushroom commonly found in East Asia including Korea, China, and Japan, widely known due to its antioxidant activity [87]. Phenols [88] and polysaccharides are the major bioactive components present in *H. marmoreus* that show the antioxidant activity by scavenging reactive oxygen species and strengthening its reducing power [55]. Liu et al. investigated the intracellular polysaccharides of *H. marmoreus* and revealed that these polysaccharides can be utilized as an antioxidant agent that improves adaptive immune reactions [89].

*L. edodes* also known as shitake mushroom is the second most well-known consumable mushroom in the worldwide market and is usually cultivated in Indonesia, Taiwan, China, and Japan [90]. The mushroom is credited to its wholesome incentive as well as to conceivable potential for therapeutic applications [91]. *L. edodes* contains some important polysaccharides that have therapeutic activity. The mushroom is also a good source of vitamins, particularly vitamin B, including B1, B2, B5, B12, and provitamin D2. *L. edodes* extract has potent antioxidant effect due to the presence of bioactive compounds ergothioneine [92]. *L. edodes* has the ability to increase the total antioxidant capacity and reduce the total oxidative stress [91]. UV-C radiation can improve scavenging capacity of *L. edodes* [93]. Chen et al. [83] separated three types of polysaccharide from fruiting bodies of *L. edodes*, and they demonstrated the polysaccharides as potent antioxidant agents that can produce healthy immune response. The treatment of mushroom

crude powder or its extract may also helpful to enhance its antioxidant capability.

and other two edible mushrooms including *L. edodes* and *Trametes versicolor* [80].

*2.3.6. Grifola frondosa*

12 Antioxidants in Foods and Its Applications

seasonal and geographic displacements.

*2.3.7. Hypsizigus marmoreus*

*2.3.8. Lentinus edodes*

*Pleurotus ostreatus* is the third most cultivated mushroom worldwide after *A. bisporus* [94]. Its mycelia as well as fruiting bodies have well-known therapeutic effects due to its various biologically active compounds including phenols, flavonoids, and carotenoids [95] having strong antioxidant activity [96]. The methanol extract demonstrated the most grounded β-carotenelinoleic acid restraint when contrasted with alternate extracts. On the other hand, acetone has the strong reducing power than alternate concentrates.

#### *2.3.10. Schizophyllum commune*

*S. commune* is a standout among the usually discovered fungus and can be separated from all landmasses, aside from Antarctica. *S. commune* has been accounted for to be a pathogen of people and trees; however, it principally receives a saprobic way of life by causing white rot. The antioxidant activity of *S. commune* is due to polysaccharides and polyphenols components [97].

## **2.4. Medicinal plants and spices having antioxidants**

#### *2.4.1. Allium sativum*

*A. sativum* commonly known as garlic is a species belongs to family Alliaceae commonly cultivated in India [98]. It is a perpetual herb with a tall, erect blooming stem that grows up to 3 feet. Garlic has been utilized all through history for both culinary and therapeutic purposes. *A. sativum* is an adaptable herb that contains various trace elements, vitamins, and minerals. The total phenolic compound of the garlic has the antioxidant activity [99]. As an antioxidant, garlic has the strongest DPPH-scavenging ability [100]. Aged garlic extract has significantly eminent total phenolic substance than raw garlic extract [101]. It has been noticed that as the plant gets older, more the antioxidant potential it will gain.

#### *2.4.2. Capsicum annuum*

*Capsicum annuum* (red pepper) is native to southern North America and northern South America and was introduced in Asia in sixteenth century from South America [102]. It contains a wide cluster of phytochemicals with their radical-scavenging properties [103]. The spice contains carotenoids, flavonoids, tocopherols, free sugars, capsaicinoids, L-ascorbic acid, and organic acids [104]. At the ripe stage, hot-dried peppers have a high bioactive substances that show huge free radical-scavenging properties such as polyphenols and carotenoids [103].

#### *2.4.3. Curcuma longa*

*Curcuma longa* is a well-known spice that has a place in the Zingiberaceae family and is a lasting herb that measures up to 1 m high with a short stem. It is circulated all through tropical and subtropical locales of the world, being generally developed in Asiatic nations [105], primarily in India and China. In Pakistan and India, it is prevalently known as Haldi. As a powder, called turmeric, it has been in continual use as a flavor enhancer in both veggies lover and non-vegan foods. Essential oil of fresh rhizomes has higher scavenging properties [106]. The phenolic compounds of *C. longa* are the primary contributor of antioxidant activity [107].

of *U. tomentosa* are generally utilized as a part of the conventional Peruvian medicine for the treatment of many diseases [119]. The bark decoctions have strong ability to decrease the free

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*Leea indica* belongs to the family Vitaceae and has been traditionally used as natural folk medicine in Malaysia. In the leaves of *L. indica*, 23 known chemical compounds are identified [120]. The identified compounds include 11 hydrocarbons, 3 phthalic acid esters, phthalic acid, gallic acid, ursolic acid, solanesol, farnesol, β-sitosterol, lupeol, and 1-eicosanol [120]. Among

*Polyalthia cerasoides* belongs to the family Annonaceae and is a medicinal plant used in Thai native medicine. The roots of *P. cerasoides* are used for therapeutic purposes that contain alkaloid, bidebiline, three known sesquiterpenes, four known isoquinoline, and other compounds such as laudanosine, codamine, laudanidine, and reticuline [122]. The extract has the highest

Marine ecosystem has been reported as a potential source of biodiversity and chemical activities. The organisms living in marine environment are gaining the attention of industries such as pharmaceuticals, nutraceuticals, and cosmetics because of possessing various interesting and useful chemical compounds [125]. Marine biotechnologists are trying to produce the tool for the utilization of marine biodiversity for the production of cheap source of pharmaceutical products and functional foods [126]. Seaweeds and sponges are considered as the richest source of bioactive compounds having the antimicrobial and antioxidant activities [127]. Seaweeds and sponges with their associated bacteria have been found to possess various health-promoting and disease prevention effects due to their phenolic compounds, polysaccharides, and useful organic acids [128]. These are supposed to be the most protective group of foods against environmental pollutants and radiation [129]. Among various other useful compounds, the marine organisms also contain polyphenolic compounds that are responsible for antioxidant activity including flavonoids, benzoic acid, cinnamic acid, gallic acid, quercy, and phlorotannins [130]. Nonanimal sulfated polysaccharides are reported to have antioxidant activities [131], which can be obtained from marine algae and other marine organisms from the phaeophyta group [132]. A large number of different species of algae and microalgae have been studied for the use of their bioactive contents as functional food components. Algae comprised of a huge and complex group of photosynthetic organisms with simple reproductive organs, which can be multicellular, known as macroalgae and unicellular called as microalgae. Algae grow in extremes of environmental conditions such as light, temperature, and salinity, which results in the production of a large number of reactive oxygen species (ROS). To cope with these ROS, algae produce various secondary metabolites with many antioxidant activities such as phycobilins,

phenolic compound and high reactive oxygen species-scavenging activity [123, 124].

radicals diphenylpicrylhydrazyl, hydrogen peroxide, and hypochlorous acid [119].

these, total phenolic compounds possess the antioxidant activity [121].

*2.4.9. Leea indica*

*2.4.10. Polyalthia cerasoides*

**2.5. Antioxidants from marine sources**

polyphenols, carotenoids, and vitamins [133].

#### *2.4.4. Eugenia caryophyllus*

*Eugenia caryophyllus* commonly known as clove is a medium-size tree (8-12 m) that belongs to family Myrtaceae. *E. caryophyllus* has been utilized for a considerable length of time as nourishment additive and for some therapeutic purposes as well [108]. Clove is local of Indonesia yet these days also cultivated in some other countries including Brazil in the province of Bahia. This plant is one of the wealthiest sources of phenolic compounds, for example, gallic acid eugenol and eugenol acetate [108]. *E. caryophyllus* leaf essential oil and its main constituent eugenol possess high antioxidant activity [109]. Among various extracts, the methanolic extract has higher scavenging activity than acetone and chloroform extracts [110].

### *2.4.5. Geranium sanguineum*

*Geranium sanguineum*, usually called as bloody cranesbill, is a herbaceous plant that belongs to family Geraniaceae. It is local from Asia and Europe and is developed as a garden subject. In Pakistan, India, Sri Lanka, Indonesia, and Zanzibar, it is cultivated on large scale. It is found naturally in Madagascar, Brazil, Sri Lanka, Tanzania, and West Indies [111]. Methanol extract of *G. sanguineum* has the free radical-scavenging property [112].

#### *2.4.6. Pistacia lentiscus*

*Pistacia lentiscus* is extensively used in folk medicine by rural populations in Algeria. The herb is imperative due to its therapeutic uses. Ethanol, ethyl acetate, aqueous, hexane, aqueous/ hexane, and chloroform extracts from the leaves of *P. lentiscus* have the radical-scavenging activity [113]. *P. lentiscus* have exceptional reducing power and strong radical-scavenging activity against DPPH [113, 114].

#### *2.4.7. Salvia officinalis*

*Salvia officinalis*, also known as garden sage, belongs to family Lamiaceae and possesses strong antioxidant property [115]. The plant is grown and cultivated in some parts of Iran. The leaves of the plant are utilized as a part of Iranian folk medicine. The antioxidant activity of the plant is due to the presence of polyphenol constituents [116]. Dried sage leaves infusion with boiling water (sage tea) is the most typical form of preparation. Sage tea contains polyphenolic constituents that possess antioxidant property and other therapeutic effects [117].

#### *2.4.8. Uncaria tomentosa*

*Uncaria tomentosa* is generally known as cat's claw and belongs to the family Rubiaceae. Its native is Amazon rainforest and other tropical territories of Central and South America. For centuries, the plant has been utilized as a part of customary practices in South America particularly in Peru. Due to its anti-inflammatory and radical-scavenging activities, the plant has been used to treat rheumatic diseases and cancer [118]. Decoctions prepared from the bark of *U. tomentosa* are generally utilized as a part of the conventional Peruvian medicine for the treatment of many diseases [119]. The bark decoctions have strong ability to decrease the free radicals diphenylpicrylhydrazyl, hydrogen peroxide, and hypochlorous acid [119].

## *2.4.9. Leea indica*

and non-vegan foods. Essential oil of fresh rhizomes has higher scavenging properties [106]. The phenolic compounds of *C. longa* are the primary contributor of antioxidant activity [107].

*Eugenia caryophyllus* commonly known as clove is a medium-size tree (8-12 m) that belongs to family Myrtaceae. *E. caryophyllus* has been utilized for a considerable length of time as nourishment additive and for some therapeutic purposes as well [108]. Clove is local of Indonesia yet these days also cultivated in some other countries including Brazil in the province of Bahia. This plant is one of the wealthiest sources of phenolic compounds, for example, gallic acid eugenol and eugenol acetate [108]. *E. caryophyllus* leaf essential oil and its main constituent eugenol possess high antioxidant activity [109]. Among various extracts, the methanolic

*Geranium sanguineum*, usually called as bloody cranesbill, is a herbaceous plant that belongs to family Geraniaceae. It is local from Asia and Europe and is developed as a garden subject. In Pakistan, India, Sri Lanka, Indonesia, and Zanzibar, it is cultivated on large scale. It is found naturally in Madagascar, Brazil, Sri Lanka, Tanzania, and West Indies [111]. Methanol extract

*Pistacia lentiscus* is extensively used in folk medicine by rural populations in Algeria. The herb is imperative due to its therapeutic uses. Ethanol, ethyl acetate, aqueous, hexane, aqueous/ hexane, and chloroform extracts from the leaves of *P. lentiscus* have the radical-scavenging activity [113]. *P. lentiscus* have exceptional reducing power and strong radical-scavenging

*Salvia officinalis*, also known as garden sage, belongs to family Lamiaceae and possesses strong antioxidant property [115]. The plant is grown and cultivated in some parts of Iran. The leaves of the plant are utilized as a part of Iranian folk medicine. The antioxidant activity of the plant is due to the presence of polyphenol constituents [116]. Dried sage leaves infusion with boiling water (sage tea) is the most typical form of preparation. Sage tea contains polyphenolic

*Uncaria tomentosa* is generally known as cat's claw and belongs to the family Rubiaceae. Its native is Amazon rainforest and other tropical territories of Central and South America. For centuries, the plant has been utilized as a part of customary practices in South America particularly in Peru. Due to its anti-inflammatory and radical-scavenging activities, the plant has been used to treat rheumatic diseases and cancer [118]. Decoctions prepared from the bark

constituents that possess antioxidant property and other therapeutic effects [117].

extract has higher scavenging activity than acetone and chloroform extracts [110].

of *G. sanguineum* has the free radical-scavenging property [112].

*2.4.4. Eugenia caryophyllus*

14 Antioxidants in Foods and Its Applications

*2.4.5. Geranium sanguineum*

*2.4.6. Pistacia lentiscus*

*2.4.7. Salvia officinalis*

*2.4.8. Uncaria tomentosa*

activity against DPPH [113, 114].

*Leea indica* belongs to the family Vitaceae and has been traditionally used as natural folk medicine in Malaysia. In the leaves of *L. indica*, 23 known chemical compounds are identified [120]. The identified compounds include 11 hydrocarbons, 3 phthalic acid esters, phthalic acid, gallic acid, ursolic acid, solanesol, farnesol, β-sitosterol, lupeol, and 1-eicosanol [120]. Among these, total phenolic compounds possess the antioxidant activity [121].

## *2.4.10. Polyalthia cerasoides*

*Polyalthia cerasoides* belongs to the family Annonaceae and is a medicinal plant used in Thai native medicine. The roots of *P. cerasoides* are used for therapeutic purposes that contain alkaloid, bidebiline, three known sesquiterpenes, four known isoquinoline, and other compounds such as laudanosine, codamine, laudanidine, and reticuline [122]. The extract has the highest phenolic compound and high reactive oxygen species-scavenging activity [123, 124].

#### **2.5. Antioxidants from marine sources**

Marine ecosystem has been reported as a potential source of biodiversity and chemical activities. The organisms living in marine environment are gaining the attention of industries such as pharmaceuticals, nutraceuticals, and cosmetics because of possessing various interesting and useful chemical compounds [125]. Marine biotechnologists are trying to produce the tool for the utilization of marine biodiversity for the production of cheap source of pharmaceutical products and functional foods [126]. Seaweeds and sponges are considered as the richest source of bioactive compounds having the antimicrobial and antioxidant activities [127]. Seaweeds and sponges with their associated bacteria have been found to possess various health-promoting and disease prevention effects due to their phenolic compounds, polysaccharides, and useful organic acids [128]. These are supposed to be the most protective group of foods against environmental pollutants and radiation [129]. Among various other useful compounds, the marine organisms also contain polyphenolic compounds that are responsible for antioxidant activity including flavonoids, benzoic acid, cinnamic acid, gallic acid, quercy, and phlorotannins [130]. Nonanimal sulfated polysaccharides are reported to have antioxidant activities [131], which can be obtained from marine algae and other marine organisms from the phaeophyta group [132].

A large number of different species of algae and microalgae have been studied for the use of their bioactive contents as functional food components. Algae comprised of a huge and complex group of photosynthetic organisms with simple reproductive organs, which can be multicellular, known as macroalgae and unicellular called as microalgae. Algae grow in extremes of environmental conditions such as light, temperature, and salinity, which results in the production of a large number of reactive oxygen species (ROS). To cope with these ROS, algae produce various secondary metabolites with many antioxidant activities such as phycobilins, polyphenols, carotenoids, and vitamins [133].

People living in coastal areas use many types of seaweed, both as fresh and dry forms, as a natural source of food, and from the research, it is known that these seaweeds contain a large amount of proteins, minerals, and vitamins. Although the composition of these seaweeds varies according to their species, geographical distribution, temperature, and seasonal variation, the overall nutritional value remains the same. Many compounds from marine algae possess anticancer activity, and recently, seaweeds have gained attention as a rich source of antioxidants [134]. Many of the secondary metabolites produced by marine organisms reflect the presence of chloride and bromide ions in seawater. Marine halogenated compounds assemble a large number of other useful compounds such as indoles, peptides, terpenes, phenols, acetogenins, and volatile halogenated hydrocarbons. This protective effect suggests the presence of antioxidant compounds that show their antioxidant activity as free radical scavengers, hydrogen-donating compounds, single oxygen quenchers, and metal ion chelators. Many biological compounds have previously isolated from some other marine organisms such as fish, crustaceans, and their byproducts [135].

focusing on the alternate sources other than the synthetic one, which will be more safe and convenient as dietary component. Although there are no such harmful reports have been observed regarding the use of synthetic antioxidants however the consumer's interest is also compelling toward the nature close products. Moreover, the synthetic antioxidants and preservatives in the food may lead to lipid peroxidation and deterioration of food flavor and quality. The use of natural herbs, spices, and plant ingredients is in practice from the ancient times and still practiced in the traditional food preparation as preservative, aroma, and flavor. This chapter is an effort to overview the potentials of various natural sources having reasonable antioxidant potential. The literature reports compiled here will be beneficial to identify the significance of various natural sources based on their antioxidant capacity, active ingredients, and geographic availability. This chapter reveals that people can prioritize their dietary habits based on the antioxidant potential and cost-effectiveness of the available source because 70–80% of the world population cannot afford the modern supplement and medicines.

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**Author details**

**References**

2011;**10**(4):221-247

Haseeb Anwar\*, Ghulam Hussain and Imtiaz Mustafa

review. Molecules. 2010;**15**(12):9252-9287

\*Address all correspondence to: drhaseebanwar@gcuf.edu.pk

Department of Physiology, Government College University, Faisalabad, Pakistan

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[2] Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: A short

[3] Valko M et al. Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology. 2007;**39**(1):44-84

[4] Anwar H et al. Effect of protein, probiotic, and symbiotic supplementation on serum biological health markers of molted layers. Poultry Science. 2012;**91**(10):2606-2613

[5] Carocho M, Ferreira IC. A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future

[6] Asif M. Chemistry and antioxidant activity of plants containing some phenolic com-

[7] Ramalakshmi K, Kubra IR, Rao LJM. Antioxidant potential of low-grade coffee beans.

perspectives. Food and Chemical Toxicology. 2013;**51**:15-25

pounds. Chemistry International. 2015;**1**(1):35-52

Food Research International. 2008;**41**(1):96-103

Seaweeds also create a suitable environment to a large number of bacteria that live on their surface having much more diversity of microorganisms as compared to other multicellular organisms [136]. These associated microorganisms have a protective effect on the seaweeds from pathogen, and they produce a large number of bioactive compounds of biomedical importance [137]. Exopolysaccharides produced by these bacterial species are used as an ingredient in food, petroleum, and pharmaceutical industries and emulsification of crude oil, vegetables, mineral oils, and bioremediation agents in environment management systems [138].

Fish protein hydrolysate (FPH), which is prepared from various marine organisms such as mackerel, tuna, Alaska Pollock, and yellowfin sole, has also been reported to have antioxidant activity [139–141]. Many types of peptides are obtained from fish muscle, bone, skin, and other tissues. All of these amino acids can scavenge-free radicals, but the most powerful scavenging activity attributes to those who can easily donate hydrogen atoms. These amino acids are cystine and methionine, which have nucleophilic sulfur-containing side chains or tryptophan, tyrosine, and phenylalanine, which have aromatic side chains. Peptide size and amino acid composition are important for the FPH because it determines its antioxidant nature [142].

An in vitro study on phycocyanin, a pigment obtained from blue-green algae, reveals its antioxidant activity. It was evaluated in vitro by the use of luminol-enhanced chemiluminescence (LCL). Luminol reacts with oxygen (O−2), alkoxyl (RO˙), and hydroxyl (OH˙) radicals and shows a luminous signal measurable before and after antioxidant addition. This antioxidant activity was also confirmed in vivo by induction of inflammation in mice paw with glucose oxidase. The edema caused by inflammation was reduced, and the luminous signal indicated that the phycocyanin can scavenge OH˙ and RO˙ [143]. Algal antioxidants are also used in the cosmeceutical industries as antiaging agents [144]. A carotenoid pigment known as astaxanthin, found in microalga *Haematococcus pluvialis*, is reported to have anti-inflammatory, immunomodulatory, and antioxidant activities [145].
