3.1. Bone marrow

BM-MSCs are isolated from bone marrow aspirate. This invasive procedure is painful for the patient with a risk of infection. The commonly used method for the generation of MSCs from bone marrow is density gradient centrifugation [3]. The collected fraction containing mononuclear cells is washed and the cells are seeded on a plastic dish for proliferation.

### 3.2. Adipose tissue

AT-MSC also termed as adipose-derived stem cells are usually isolated from the biological material generated during liposuction, lipoplasty or lipectomy procedures by enzymatic digestion with collagenase followed by centrifugation and washing [5].
