**2.4. Detection of SAβGalA and cell cycle analysis**

SAβGalA was measured using the median fluorescence intensity (MFI) as previously reported [45]. Co-cultures of HD-BMMSC and HS-5 stromal cells (CRL-11882) were used as controls. In addition, β-galactosidase activity was analyzed using the "Senescence Cells Histochemical Staining Kit" (Sigma-Aldrich) as recommended by the manufacturer. Cell cycle analysis was performed using the "Cell Cycle Assay Kit" (Abcam) as recommended in the prescription. Data were studied using a logarithmic scale.
