3.3. Peripheral blood

Following a density gradient centrifugation, PB-MSC can be collected from the mononuclear cells' lymphocyte separation fluid fraction. Kassis et al. [6] described another method, which is loading PB-MSC on fibrin microbeads, then separating the cell loaded beads from the mononuclear fraction. This method allows getting enormous amounts of MSCs [6].

Pittenger et al. [3], isolated MSCs from BM by density gradient centrifugation to eliminate unwanted cell types and only 0.001 to 0.01% of the cells isolated from the density interface were identified as mesenchymal stem cells.
