**4. Electrophoretic techniques used for separation of LDH isoenzymes**

Lactate dehydrogenase isoenzymes can be separated using various supporting media such as starch, agarose, cellulose acetate, and polyacrylamide gel. The separations should be carried out with cooling to 4°C in order to prevent the possibility of enzyme inactivation, particularly heat-sensitive slow-moving isoenzymes (LDH4 and LDH5 ) [3].

Two types of polyacrylamide gel electrophoresis for the separation of lactate dehydrogenase isoenzymes of mammalian and bird origin were used in our laboratory (PhastSystem, Pharmacia LKB, Sweden):

