**Methylated region (M: methylated):**

M-Forward: ATGTTGAAGAAAATGAAATTTTCGT,

M-Reverse: ACTTATCCCCGATAAAATCCG,

PCR product: 164 base pairs (bp).

**Unmethylated region (UnM: unmethylated):**

UnM-Forward: TTTATTTTTAGAAGGTTGTGG,

UnM-Reverse: AAACTACCAAACAAAAAACAACATC,

PCR product: 163 bp.
