*3.7.3. Alkaline phosphatase activity (ALP)*

Preservation of the osteoblastic phenotype in OB cells was assayed using ALPase activity. It was observed that all CPC groups had a strong ALPase activity showing the preservation of the osteoblast phenotype (**Figure 10**). From the graph, it can be inferred that the cells had enhanced ALPase activity on day 14 compared to day 7. A pronounced increase in ALPase activity was seen in CPC discs without HNTs and CPCs with 10% wt. HNTs. There was, however, no statistical difference among groups on a given day of assay. All histochemical staining results suggest that CPC surfaces are conducive for osteoblast attachment, proliferation, and synthesis of an organic matrix that was subsequently mineralized. Osteogenesis and cell viability are the critical requirements for bone tissue regeneration.
