**Acknowledgements**

through outputs. Similarly different shapes of particles like non-spherical or rod-type shape can be utilized and detected through microchannel network for sorting. By this system of sieve network it achieves a high throughput sorting while separation occurred at every inter-

Micro device having sieve-shaped micropatterned channel has been designed and simulated for a distinct slanted angles at 90, 15, 25 and 35° through which separation of mass and size-based particles from a sample was carried for *K. pneumoiae* beads (by using its properties). The model for the Sieve type microchannel has been done based on the equation for critical diameter, Navier-stokes equations analysis and accordingly depending on the mass and size of the particle it was separated through the main and separation channels of micropatterned chip of sieve type model. The fluid flow simulation was tested with blood sample and distilled water properties in simulation at different angles of channels by which a bigger particle as well as smaller particles were separately identified via outputs for the precise separation (**Figures 11** and **12**). The main channel and the separation channel were crossing at right angle and also the particles with the size of 3 and 8 μm (aspect ratio = 0.3) were separated through outputs at various flow rates in μl/s for blood and water mixed with properties of *K. pneumoniae* beads by micro channel network as the size of the *K. pneumoniae* varies from 0.5 to 2.5 μm shown in **Figure 11**.

We examined the model of separation at different input flow rates concerning about 13, 20, 25, 50, 66, 80 μl/s with water and 133, 666, 800, 1000, 3000, 5000 μl/s with blood by which the separation behavior of the particles using COMSOL Multiphysics 5.2a were simulated and calculation is done. Similarly, at various angles of the channels the samples through input were simulated for the quick and precise performance for the sorting of bacteria at different

Finally a discussion can be made for the *K. pneumoniae* particles that it can be examined with different size of the spherical or non-spherical shape cells for separation which has been a big issue in hospitals and routine lifestyle. Since infections *K. pneumoniae* are air borne type which is controlled by mucosal vaccination through pulmonary route and nasal can be an assured approach in order to resemble natural infection. Other than traditional vaccines new vaccines like subunit vaccines are safer but they have less immune response [30]. Therefore to enlarge their immunogenicity, dynamic and delivery systems and safe adjuvant are necessary to be developed. Thus by using micropatterning system the sorting of particle from samples can be easily invented [30]. Furthermore this microchannel network can be carried for different purposes for biological or industrial work and even for the separation of rod-type particles from the *K*. *pneumoniae* by improving separation efficiency and change or modification in design

There was an good development in past, where various microfluidic techniques were developed which involves application in enrichment, particle sorting and detection. However

fluid flow rates mentioned shown in **Figure 12**.

86 MEMS Sensors - Design and Application

for the better cause.

**4. Conclusion**

section and is robust against the issue of clogging in microchannel network [6].

The authors would greatly acknowledge the support from National Institute of Technology Nagaland, Chumukedima, Dimapur, India and MEMs Design Centre from SRM university, Chennai, Tamil Nadu, India for their assistance.
