**4. Conclusion**

There was an good development in past, where various microfluidic techniques were developed which involves application in enrichment, particle sorting and detection. However expected high throughput and clogging problem are the facts which has to be properly experimented for higher purpose of biological testings, their conditioning and patterning related fabrication techniques for developement with some characteristics and precise separation of cells should be one of the top priorities. Thus sieve-typed microchannel network can be a precise separation chip network which is efficient for hydrodynamic cell in microfluidic device of size and mass based separation. Effective for mucosal vaccine delivery systems, while flow can be related for immune protection against *K. pneumoniae* infection and suitable for group vaccination programs resulting in considerable health as well as economic benefits. Therefore to contribute to the different successful collaborations at this stage where the stability and the robustness of the microfluidic viability should be taken into care to steadily contribute to successful collaborations between different biomedical, clinicians, biologists, and other the microfluidic association.
