**4. Experiment and results**

Micro-injection flow channel design parameters monomer, the impact of the ejection orifice thickness also caused performance is very important, its impact may be the effect of differences in both exit velocity of the droplets and flight direction. It is necessary to know the thickness of the sheet discharge orifice (nozzle plate thickness) affect the size ratio between the length of the two nozzle diameter caused respect. Through computer simulation can be obtained as shown in **Figure 5**. Simulation of DNA in the orifice thickness reduced from 50 to 25 μm single-aisle jet chamber. The results can be seen due to the reduced thickness of the orifice. Droplet flight directionality deteriorated. The reason is that the effect of the thickness of the orifice having rectified so that the flow field velocity with consistent directionality. But too thick so that it will produce additional drag exports slowed down. Orifice having a different orifice diameter should be of different sheet thicknesses. The calculation results are in 200 μs moment, obviously not yet fluid backfill is completed. It causes of reduced thickness orifice, causing the fluid velocity to large-amplitude reduction backfilling, because the droplet from the nozzle holes. Droplet tail flight directionality deteriorated. It has already begun to overflow orifice surface side. Resulting is in resistance, due to surface tension forces led by the great surface. Prevents fluid filled into the interior of the cavity injection phenomenon.

direction from the channel inlet cavity injection, such as speed XY sectional flow field in an instant 200 μs shown can clearly see resistance to the flow field, it is resulting in increased

**Figure 5.** The simulation of DNA was calculated on a three-dimensional top view of (a) a three-dimensional side view (b) at 10, 20, 30, 40 and 200 μs at a single channel ejection cavity with a hole diameter of 60 μm and a thickness of 25 μm

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**Figure 7** is a schematic diagram of a cross-sectional view of a precise overall DNA jet multidimensional drive addressing chip. The amount of each single point DNA sequencing (spray volume) is determined by the magnitude of the amplitude power. DNA trace differences can be adjusted. Heater also increases the number of pads as the number of thermal resistance components increases. DNA jetting system costs also increase proportionally. Therefore, Heater thermal resistance components must increase the number of logic multiplexed control circuit. It integrates the related process technology. Making the appropriate driver circuit and selective switching circuit can dramatically reduce the required number of external Pads and

velocity of the fluid backfill. **Figure 6** is shown Droplet generation control simulation.

reduce manufacturing costs.

at an operating frequency of 5 KHz spray situation.

Single injection process in real time, and cannot ensure just above the orifice sheet ejection orifice center is located in the center of the cavity, it is necessary to simulate the effect of offcenter position when the spray hole caused by the analysis. The results show that the ejected by the droplet and will not deviate from the center position of the nozzle holes, caused by large droplets tails flying directional radiation deteriorated. The results show the fluid actually increased after the injection backfilling operation speed, almost complete backfill to the surface of the nozzle holes, the reason may be because the orifice is placed away from the channel inlet of the injection cavity side wall surface side so that the fluid this reduces while reducing internal flow field reflux (circulation) from happening; directly back to fill in one Precisely Addressed (DNA Gene) Spray Microfluidic Chip Technology http://dx.doi.org/10.5772/intechopen.74611 209

mother. Each person cut the fragment should be at least one of both parents the same. As long

In recent years, due to the development of polymerase chain reaction (PCR) technology. DNA identification technology uses PCR to amplify the sample DNA collected. It selects mini-satellites DNA with shorter repeat fragments in the VNTR as the target of the assay. Single-parent paternity tests can also be confirmed using maternally inherited mitochondrial DNA or paternally inherited Y chromosomes. Sperm contained in the mitochondria body is very small, most of the mitochondria within the fertilized egg from the egg. It compares the mitochondrial DNA in the offspring cells to determine if the relationship is mother-daughter, mother-daughter, or sibling. The male Y chromosome did not participate in synapsis or gene recombination during meiosis. It therefore determines the relatives of father, son, brother or paternal relative to the Y chromosome. These can be used as a paternity test to

Micro-injection flow channel design parameters monomer, the impact of the ejection orifice thickness also caused performance is very important, its impact may be the effect of differences in both exit velocity of the droplets and flight direction. It is necessary to know the thickness of the sheet discharge orifice (nozzle plate thickness) affect the size ratio between the length of the two nozzle diameter caused respect. Through computer simulation can be obtained as shown in **Figure 5**. Simulation of DNA in the orifice thickness reduced from 50 to 25 μm single-aisle jet chamber. The results can be seen due to the reduced thickness of the orifice. Droplet flight directionality deteriorated. The reason is that the effect of the thickness of the orifice having rectified so that the flow field velocity with consistent directionality. But too thick so that it will produce additional drag exports slowed down. Orifice having a different orifice diameter should be of different sheet thicknesses. The calculation results are in 200 μs moment, obviously not yet fluid backfill is completed. It causes of reduced thickness orifice, causing the fluid velocity to large-amplitude reduction backfilling, because the droplet from the nozzle holes. Droplet tail flight directionality deteriorated. It has already begun to overflow orifice surface side. Resulting is in resistance, due to surface tension forces led by the great surface. Prevents fluid filled into the interior of the cavity injection

Single injection process in real time, and cannot ensure just above the orifice sheet ejection orifice center is located in the center of the cavity, it is necessary to simulate the effect of offcenter position when the spray hole caused by the analysis. The results show that the ejected by the droplet and will not deviate from the center position of the nozzle holes, caused by large droplets tails flying directional radiation deteriorated. The results show the fluid actually increased after the injection backfilling operation speed, almost complete backfill to the surface of the nozzle holes, the reason may be because the orifice is placed away from the channel inlet of the injection cavity side wall surface side so that the fluid this reduces while reducing internal flow field reflux (circulation) from happening; directly back to fill in one

as it compares to the three-way RFLP, it can confirm the parent-child relationship.

determine the relevant evidence.

208 Microfluidics and Nanofluidics

**4. Experiment and results**

phenomenon.

**Figure 5.** The simulation of DNA was calculated on a three-dimensional top view of (a) a three-dimensional side view (b) at 10, 20, 30, 40 and 200 μs at a single channel ejection cavity with a hole diameter of 60 μm and a thickness of 25 μm at an operating frequency of 5 KHz spray situation.

direction from the channel inlet cavity injection, such as speed XY sectional flow field in an instant 200 μs shown can clearly see resistance to the flow field, it is resulting in increased velocity of the fluid backfill. **Figure 6** is shown Droplet generation control simulation.

**Figure 7** is a schematic diagram of a cross-sectional view of a precise overall DNA jet multidimensional drive addressing chip. The amount of each single point DNA sequencing (spray volume) is determined by the magnitude of the amplitude power. DNA trace differences can be adjusted. Heater also increases the number of pads as the number of thermal resistance components increases. DNA jetting system costs also increase proportionally. Therefore, Heater thermal resistance components must increase the number of logic multiplexed control circuit. It integrates the related process technology. Making the appropriate driver circuit and selective switching circuit can dramatically reduce the required number of external Pads and reduce manufacturing costs.

of the amplitude and power, DNA trace differences can be adjusted action. For the same DNA sequence of the password, to be able to get different lengths of continuous fragments, and know the end of these fragments corresponding to the A, T, C, G base. Thus, as long as the length of these fragments ordered, the original DNA sequence of the complete base to know. As for the method of obtaining a large number of fragments; after a large amount of DNA to be sequenced is copied first, the DNA is cut at different bases. For example, the restriction will cut off double helical DNA at specific base alignments. The effect of cleaving DNA at specific bases can also be achieved by chemical reactions that remove bases. Also, copy the DNA that used to contain the complete passcode as a template. It is randomly interrupted to interrupt without growth. It produces a variety of pieces of different lengths. It sorts DNA fragments of different lengths. It is a technique called gel electrophoresis. Gel electrophoresis can distinguish small differences in DNA fragments that have been cut off. The rationale is that the flow rate of a molecule fragment in the gel is inversely proportional to the logarithm of the number of base pairs. The shorter the molecular fragment, the faster it will drift in the colloid. Gel electrophoresis on the length of fragment resolution is very high. It is for two DNA fragments that themselves are hundreds of units long. The difference between the length of only one nucleotide can be distinguished.

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**Figure 8.** Open pool printhead chip.

This systematic phase verification follows the DNA droplet addressing requirements. It is the first plan to send signals to the output thermal resistance module, as shown in **Figure 8**. Each thermal resistance has a corresponding signal and open pool measurement. It allows this signal is generated after the designated system contains quantitative circuits. It can adjust the voltage level of the output circuit to accurately quantify DNA. Adjustable jet emits DNA flow through the system wafer. DNA jetting system wafer characteristics measurement, pulse width modulation (PWM) power corresponding quantitative DNA gene-matching parameters, high voltage power transistor driver design compensation. It optimizes the parameters of power components to observe the changes of DNA beads under different operating condi-

In the biomedical micro-liquid bead quantitative, the integrity and cleanliness of the liquid bead is often about the accuracy of quantitative or not. Hot bubble bead generation, will produce a series of beads. It is in addition to the main liquid beads, there are satellite beads

tions. The DNA liquid tube array and droplets on the carrier is shown in **Figure 9**.

**Figure 6.** Droplet generation control simulation.

The original voltage source is a group of DC voltage drop. The voltage divider is divided into eight groups by nine resistors, and one group of voltages is selected by three pairs of eight decoders. This circuit architecture can convert the original 5 V logic pulse into the voltage required by the circuit system, and output the required voltage through the PN transistor, Smooth error compensation process, to achieve the amount of liquid DNA is determined by the size

**Figure 7.** A schematic diagram of a cross-sectional view of a precise overall DNA jet (the merging of multi-dimensional data registration circuit and DNA jet technologies).

**Figure 8.** Open pool printhead chip.

The original voltage source is a group of DC voltage drop. The voltage divider is divided into eight groups by nine resistors, and one group of voltages is selected by three pairs of eight decoders. This circuit architecture can convert the original 5 V logic pulse into the voltage required by the circuit system, and output the required voltage through the PN transistor, Smooth error compensation process, to achieve the amount of liquid DNA is determined by the size

**Figure 7.** A schematic diagram of a cross-sectional view of a precise overall DNA jet (the merging of multi-dimensional

**Figure 6.** Droplet generation control simulation.

210 Microfluidics and Nanofluidics

data registration circuit and DNA jet technologies).

of the amplitude and power, DNA trace differences can be adjusted action. For the same DNA sequence of the password, to be able to get different lengths of continuous fragments, and know the end of these fragments corresponding to the A, T, C, G base. Thus, as long as the length of these fragments ordered, the original DNA sequence of the complete base to know. As for the method of obtaining a large number of fragments; after a large amount of DNA to be sequenced is copied first, the DNA is cut at different bases. For example, the restriction will cut off double helical DNA at specific base alignments. The effect of cleaving DNA at specific bases can also be achieved by chemical reactions that remove bases. Also, copy the DNA that used to contain the complete passcode as a template. It is randomly interrupted to interrupt without growth. It produces a variety of pieces of different lengths. It sorts DNA fragments of different lengths. It is a technique called gel electrophoresis. Gel electrophoresis can distinguish small differences in DNA fragments that have been cut off. The rationale is that the flow rate of a molecule fragment in the gel is inversely proportional to the logarithm of the number of base pairs. The shorter the molecular fragment, the faster it will drift in the colloid. Gel electrophoresis on the length of fragment resolution is very high. It is for two DNA fragments that themselves are hundreds of units long. The difference between the length of only one nucleotide can be distinguished.

This systematic phase verification follows the DNA droplet addressing requirements. It is the first plan to send signals to the output thermal resistance module, as shown in **Figure 8**. Each thermal resistance has a corresponding signal and open pool measurement. It allows this signal is generated after the designated system contains quantitative circuits. It can adjust the voltage level of the output circuit to accurately quantify DNA. Adjustable jet emits DNA flow through the system wafer. DNA jetting system wafer characteristics measurement, pulse width modulation (PWM) power corresponding quantitative DNA gene-matching parameters, high voltage power transistor driver design compensation. It optimizes the parameters of power components to observe the changes of DNA beads under different operating conditions. The DNA liquid tube array and droplets on the carrier is shown in **Figure 9**.

In the biomedical micro-liquid bead quantitative, the integrity and cleanliness of the liquid bead is often about the accuracy of quantitative or not. Hot bubble bead generation, will produce a series of beads. It is in addition to the main liquid beads, there are satellite beads

[5] Lin H-B, Huston AL, Justus BL, Campillo AJ. Some characteristics of a droplet whisper-

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**Figure 9.** Multiplexer DNA solution jet part.

followed. It has a considerable degree of impact on accurate quantitation. In this study, a complete master bead was used and the beads were quantitatively accurate. It meets the biomedical specimen liquid bead quantitative requirements. The study was used to create DNA microarray chips for disease detection and gene sequencing.
