**Microarrays as Platform for Multiplex Assays in Biomarker and Drug Discovery**

Pablo Juanes-Velasco, Javier Carabias-Sanchez, Rodrigo Garcia-Valiente, Jonatan Fernandez-García, Rafael Gongora, Maria Gonzalez-Gonzalez and Manuel Fuentes

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.75614

### **Abstract**

Despite the tremendous advances in the understanding of the molecular mechanisms and the complexity of the diseases is one of the present challenges for the scientific community; then, novel strategies are required to be designed and developed for effective strategies for early diagnosis and treatment. As many cellular alterations are observed at protein level, high-throughput assays are dramatically needed for biomarker discovery. Herein, we describe advantages and limitations of protein microarrays, as proteomics strategy useful for multiplex and high-throughput protein characterization in clinical samples. Finally, a few examples are discussed; mostly of them related to currently disease biomarkers already identified in proximal fluids by protein arrays are discussed.-

**Keywords:** biomarker discovery, multiplex detection, protein microarrays
