**6.2. Vaccine candidates**

In 2014, the firstly reported study about pathogen-host protein interactions by CID and workers. In this work, they studied the presence of post-transcriptional modifications in effector proteins, T3SS proteins, from different mutants of *Salmonella typhimurium* when they infected *in vitro* Hela. Lysate collection representing all infection conditions are printed and using several validated antibodies, they show a comparative results among the different assays according to abundance proteins or post-transcriptional modifications [25].

Another similar study, performed by Li and coworkers, has been published about identification of 149 antigens from *Yersinia pestis* presented in the serum from EV76 rabbit and to test the immunogenicity of several viral proteins [26].

In 2009 Thanawastein etal. developed an approached called Expressed protein screen for immune activators (EPSIA) which were successfully applied in the identification of novel bacterial immunostimulatory proteins from *Vibrio cholerae* [27].

Recently, Manzano et al. reported a set of novel vaccine candidates for *O. moubata* based on a systematic characterization of >2000 host-pathogen interactions, which were evaluated with self-assembled protein microarrays based on a cDNA library encoding >400 recombinant proteins of *O. moubata* [28].

In a most recent study, Montor etal. describe a work using NAPPA arrays to evaluate- candidate membrane antigens in *P. aeruginosa* which could help to track the immune responses of patients infected with *P. aeruginosa* and healthy ones. In this work, 12 proteins have been identified being mostly of them related with adaptive immune response- in infected patients [29].
