**7. Conclusions and further directions**

Here, we have briefly reviewed protein microarray field as suitable platform for multiplex assays in high-throughput format. Thus, the focus was on two main perspectives: (i) Key technological aspects, (ii) Biological Applications.

However, as we described previously, despite the fundamental advances in protein microarrays, allowing characterization of whole human proteome is still remaining as a challenge. Then, the information provides light on the functions of proteins and genes whose functions are currently unknown.

Overall, protein arrays may provide relevant information about the biological function of gene products. Although, it is still necessary to develop and optimize some key aspects of protein microarray in the future, other proteomics approaches could provide complimentary results.
