**2.10.2 Method**

1. Two sets of four tubes were labeled as follows: B-reagent blank, standard (2mmol/l)

C - Control; p - patient.

	- B. 0.05mls distilled water
	- S 0.05mls standard (2mmol/1)
	- C- 0.05mls Control serum
	- p 0.05mls patient serum

The contents of each tube were mixed for several seconds.


$$\text{Ca}^{2+} \text{ (mmol1/L)} = \frac{\text{AT}}{\text{AS}} \times 2 \text{ }^{\\}$$

AT= Absorbance of test or control

AS= Absorbance of standard

Serum calcium values of 8.5 – 10.5mg/dl was taken as normal.

#### **2.11 Measurement of serum alkaline phosphatase**

This was done using the kind and king method (1954). This is a standard colorimetric method.
