**8. Effects of HE-86 administration on urine TGF**-**β1**

High excreation of urine TGF-β1, which express both glomerular and tubulointerstitial injuries. To demonstrate further the anti-inflammatory effect of HE-86 on rat chronic renal failure, we determined the TGF-β1 levels within the urine by ELISA. Results demonstrated that compared with vehicle, He-86 treatment significantly reduced urinary TGF-β1 levels, corrected by decrease level of serum creatinine, throughout the entire disease course (P<0.05), indicating that HE-86 treatment may primarily suppress the local immune and inflammatory response within the diseased kidney. In contrast, overexpression of urine TGF-β1 was found in control uraemic rats as compared with normal rats (Table 5). The experimental result showed the administration of HE-86 significantly inversed high expression of urine TGF-β in uraemic rats, manifesting HE-86 to attenuate the development of glomerular sclerosis.


Table 5. Effect of HE-86 liquid extract on urine TGF-β excretion in 5/6 nephrectomy in rats. (#P<0.05, ##P<0.01,when compared against empty vector-treated controls; \*P<0.05, \*\*P<0.01, when compared to normal sham-controls)
