**7. Effects of HE-86 administration on mean arterial blood pressure in uraemic rats**

After subtotal nephrectomy, hypertension developed in both HE-86 treatment and control uremic rats. Blood pressure was significantly elevated from second to eighth week after nephrectomy compared to sham-operated animals (P < 0.05-0.01), and the rise in blood pressure was equivalent (systolic blood pressure 180 to 200 mmHg) in control group. After using HE-86 liquid extract, hypertension was obviously suppressed in treatment group, showing average systolic blood pressure 140 to 160 mmHg (Table 4).

AT1R activation, TNF–α expression and NF-κb accumulation in sham, control and HE86 treated animals were assessed by one-way analysis of variance, followed by t-test. Results

Renal and systemic parameters obtained at 0 (before treatment), 32and 64 days after Nx are given in Table 1-5, Figure 1-5. Nx groups exhibited limited growth compared with Sham. In all Nx groups except treatment group, body weights were statistically different from those

**6. Effects of HE-86 administration on biochemical parameters in uraemic rats**  Table2-3 shows the summary of renal function and 24h urine protein level. There was significant change in body weight between the control uraemic (control) and HE-86 treated uraemic (treatment) rats, although they were pair-fed. body weight of treatment group was showed more than control uraemic. Even 4 weeks after 5/6-nephrectomy, the levels of serum creatinine and BUN were markedly increased as compared to sham rats. Not only at 4 week but also at 8 week, the uraemic rats treated with HE-86 were manifested significantly decreased levels of serum creatinine, BUN, respectively. Urinary protein excreation was also

were considered statistically significant when the P value was <0.05.

observed before treatment. Average food intake was similar among groups.

suppressed obviously at 8 week as comparing with control uraemic rats.

##P<0.01,when compared against empty vector-treated controls

 N BUN(mmol/L) Scr(μmol/L) sham 12 6.790.70 26.251.04 control 12 12.093.37 50.5615.83 treatment 12 9.812.93 38.8312.00#

sham 12 9.31±1.05 18.88±1.55 22.34±4.4 control 12 14.85±2.83 53.38±12.05 41.47±8.07 treatment 12 13.62±2.81 41.00±10.51## 29.14±5.68##

showing average systolic blood pressure 140 to 160 mmHg (Table 4).

Table 2. Serum creatinine and blood urea nitrogen after 4 week treatment. #P<0.05,

N BUN(mmol/L) Scr(μmol/L) 24h urine protein(mg)

Table 3. Serum creatinine, blood urea nitrogen and twenty-four-hour urinary protein excretion after 8 week treatment. #P<0.05, ##P<0.01,when compared against empty vector-

**7. Effects of HE-86 administration on mean arterial blood pressure in uraemic** 

After subtotal nephrectomy, hypertension developed in both HE-86 treatment and control uremic rats. Blood pressure was significantly elevated from second to eighth week after nephrectomy compared to sham-operated animals (P < 0.05-0.01), and the rise in blood pressure was equivalent (systolic blood pressure 180 to 200 mmHg) in control group. After using HE-86 liquid extract, hypertension was obviously suppressed in treatment group,

**5. Result** 

treated controls

**rats** 


Table 4. Systolic blood pressure. Data represent the means ± SEM for groups of twelve rats treated with either HE-86 or empty vector (#P<0.05,##P<0.01,when compared against empty vector-treated controls;\*P<0.05,\*\*P<0.01, when compared to normal sham-controls).
