**3. Conclusion**

In conclusion, HBOT is an interesting novel medical tool with wide range of therapeutic potential.

*In vitro* cellular models utilize different HBOT protocols and need to be standardized to bring translatable data to clinicians.

This chapter outlined that HBOT increases endothelial and fibroblast viability and proliferation *in vitro*. Furthermore, tube formation and wound healing assay improved in response to HBOT. HBOT has significant effects on endothelial-related blot-clotting and platelet mechanisms as well. Furthermore, HBOT decreases ROS-related harm in not-healing wound model and improves blood-brain barrier after ischemic event in *in vitro* model. In new therapeutic promises, the stem cells would also benefit from HBOT in maintenance, proliferation and tissue engineering aspects as well.
