**Glycoside Hydrolases in Plant Cell Wall Proteomes: Predicting Functions That Could Be Relevant for Improving Biomass Transformation Processes Predicting Functions That Could Be Relevant for Improving Biomass Transformation Processes**

**Glycoside Hydrolases in Plant Cell Wall Proteomes:** 

DOI: 10.5772/intechopen.73181

Maria Juliana Calderan-Rodrigues, Juliana Guimarães Fonseca, Hélène San Clemente, Carlos Alberto Labate and Elisabeth Jamet Juliana Guimarães Fonseca, Hélène San Clemente, Carlos Alberto Labate and Elisabeth Jamet Additional information is available at the end of the chapter

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.73181

Maria Juliana Calderan-Rodrigues,

#### **Abstract**

[103] Koutinas AA, Chatzifragkou A, Kopsahelis N, Papanikolaou S, Kookos IK. Design and techno-economic evaluation of microbial oil production as a renewable resource for biodiesel and oleochemical production. Fuel. 2014;**116**:566-577. DOI: 10.1016/j.

[104] Johnson EA. Biotechnology of non-*Saccharomyces yeasts*—The basidiomycetes. Applied Microbiology and Biotechnology. 2013;**97**(17):7563-7577. DOI: 10.1007/s00253-013-5046-z

[105] Alipour S, Habibi A, Taavoni S, Varmira K. β-carotene production from soap stock by loofa-immobilized *Rhodotorula rubra* in an airlift photobioreactor. Process Biochemistry.

[106] Cardoso LAC, Jäckel S, Karp SG, Framboisier X, Chevalot I, Marc I. Improvement of *Sporobolomyces ruberrimus* carotenoids production by the use of raw glycerol.

[108] Van Bogaert IN, Zhang J, Soetaert W. Microbial synthesis of sophorolipids. Process

[109] Shoeb E, Akhlaq F, Badar U, Akhter J, Imtiaz S. Classification and industrial applica-

[110] Vaishnav P, Demain AL. Unexpected applications of secondary metabolites. Biotechnology Advances. 2011;**29**(2):223-229. DOI: 10.1016/j.biotechadv.2010.11.006

[111] Doherty WO, Mousavioun P, Fellows CM. Value-adding to cellulosic ethanol: Lignin polymers. Industrial Crops and Products. 2011;**33**(2):259-276. DOI: 10.1016/j.

[112] Zhang YHP Reviving the carbohydrate economy via multi-product lignocellulose biorefineries. Journal of Industrial Microbiology and Biotechnology. 2008;**35**(5):367-375.

[113] Holladay J E, White J F, Bozell J J, Johnson D. Top Value Added Chemicals from Biomass-Volume II, Results of Screening for Potential Candidates from Biorefinery Lignin (No. PNNL-16983). Pacific Northwest National Lab.(PNNL), Richland, WA (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States). Available from: https://www.energy.gov/sites/prod/files/2014/03/f14/pnnl-16983.pdf [Accessed:

[114] Liu W, Zhou R, Goh HLS, Huang S, Lu X. From waste to functional additive: Toughening epoxy resin with lignin. ACS Applied Materials Interfaces. 2014;**6**(8):5810-5817. DOI:

[115] Dong T, Knoshaug EP, Pienkos PT, Laurens LM. Lipid recovery from wet oleaginous microbial biomass for biofuel production: A critical review. Applied Energy.

2016;**177**:879-895. DOI: 10.1016/j.apenergy.2016.06.002

Biochemistry. 2011;**46**(4):821-833. DOI: 10.1016/j.procbio.2011.01.010

tions of biosurfactants. Academic Research International. 2013;**4**(3):243

Bioresource Technology. 2016;**200**:374-379. DOI: 10.1016/j.biortech.2015.09.108 [107] Mata-Gómez L C, Montañez J C, Méndez-Zavala A, Aguilar C N. Biotechnological production of carotenoids by yeasts: An overview. Microbial Cell Factories. 2014;**13**:12.

2017;**54**:9-19. DOI: 10.1016/j.procbio.2016.12.013

DOI: 10.1186/1475-2859-13-12

indcrop.2010.10.022

2017-12-02]

10.1021/am500642n

DOI: /10.1007/s10295-007-0293-6

fuel.2013.08.045

164 Advances in Biofuels and Bioenergy

Glycoside hydrolases (GHs) are enzymes that are able to rearrange the plant cell wall polysaccharides, being developmental- and stress-regulated. Such proteins are used in enzymatic cocktails for biomass hydrolysis in the second-generation ethanol (E2G) production. In this chapter, we investigate GHs identified in plant cell wall proteomes by predicting their functions through alignment with homologous plant and microorganism sequences and identification of functional domains. Up to now, 49 cell wall GHs were identified in sugarcane and 114 in *Brachypodium distachyon*. We could point at candidate proteins that could be targeted to lower biomass recalcitrance. We more specifically addressed several GHs with predicted cellulase, hemicellulase, and pectinase activities, such as β-xylosidase, α and β-galactosidase, α-N-arabinofuranosidases, and glucan β-glucosidases. These enzymes are among the most used in enzymatic cocktails to deconstruct plant cell walls. As an example, the fungi arabinofuranosidases belonging to the GH51 family, which were also identified in sugarcane and *B. distachyon*, have already been associated to the degradation of hemicellulosic and pectic polysaccharides, through a peculiar mechanism, probably more efficient than other GH families. Future research will benefit from the information available here to design plant varieties with self-disassembly capacity, making the E2G more cost-effective through the use of more efficient enzymes.

**Keywords:** *Brachypodium distachyon*, cell wall proteomes, glycoside hydrolase (GH), second-generation ethanol, sugarcane

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