**3. Conclusions**

In conclusion, all the five samples used in this experiment have antibacterial activity against gram positive and negative bacteria. Gram-negative bacteria appear more resistant than Grampositive bacteria. Gram-positive and Gram-negative bacteria showed different sensitivities to essential oil. Among these samples antibacterial activity of *E. uniflora* is stronger than the others (300 μg and 600 μg, strong level against Gram-positive and negative bacteria, respectively). *S. paniculata* show the same response either to Gram-positive or Gram-negative bacteria (1500 μg), except for *P. aeruginosa* FNCC 0063 (4000 μg). Antibacterial activity of *C. aurantifolia* Swingle (Lime) is weaker than the other especially against *E. coli* (5000 μg). Five essential oils of aromatic Indonesian herbs in this study are potential candidates as antibiotic/antibacterial agents, can be applied as flavoring and preservative agents in cosmetic and food industry.
