3.2. Comparative genomics of Fusarium species complex (FSC)

orange at the top as it aged, while it was initially white at the bottom which later changed into a yellow color. The fungus is distributed throughout the world but predominant in humid

Fusarium proliferatum is grouped in FFSC and can be found on a wide host range as well as pathogenic on various agricultural crops. F. proliferatum is a common pathogen infecting numerous crop plants and occurring in various climatic zones. It occurs worldwide as a moderately aggressive pathogen of multiple plant species. F. proliferatum is well documented as a fumonisinproducing species, and some strains can produce large quantities of fumonisins, a group of polyketide-derived mycotoxins. F. proliferatum causes diseases on a remarkably wide range of plant species, including asparagus, banana, date palm, fig, mango, pine, and sorghum. F. proliferatum causing sugarcane pokkah boeng disease was firstly detected in 2012 in China. F. proliferatum in sugarcane is important for resistance, for estimating the evolutionary risk of the

During winter or in dry periods, F. proliferatum survives in the soil and on plant debris. It also produces other mycotoxins, including beauvericin, enniatins, fusaric acid, fusarin, fusaproliferin, and moniliformin. F. proliferatum can be distinguished from other species of the FFSC by analysis of molecular markers. Most recent assessments of fungal pathogens have used multilocus markers to detect populations. The ability of strains and species from geographically separated locations to recombine poses the danger of introducing virulence or toxigenic genes into local pathogen populations. The most commonly observed in human infections are F. proliferatum. However, members of FFSC are increasingly identified in especially invasive and disseminated infections in hemato-oncological patients. Many environmental Fusarium species and the human infections they cause have a worldwide distribution. The knowledge of the genetic structure of the F. proliferatum populations might be useful in order to establish effective strategies for

Other FFSC, viz., F. sacchari, F. verticillioides, F. proliferatum, and F. subglutinans, have been isolated from sugarcane. The fungus F. sacchari grows on decaying plant material and produces a large number of conidia that are spread by wind and rain. The stem borer D. saccharalis was shown to carry the fungus from plant to plant in different locations and provide access of conidia in the wind and rain to the inner stem, through their damage made to the stalk. Sugarcane infestation by the stem borer E. saccharina is a major problem in the sugar industry. The lepidopteran's infestation of sugarcane by boring the stalk rind permits Fusarium species access to the stem tissue. As a result, E. saccharina infestation is usually associated with

Fusarium oxysporum is one of the most economically important pathogens in the genus, but members of this species complex are generally considered to be non-toxigenic. F. oxysporum comprises over 120 known strains or "special forms," each of which is specific to a unique

tropical and subtropical regions and also present in the temperate regions.

144 Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

pathogen, and for planning the agricultural management practices.

3.1.3. The other members of Fusarium fujikuroi species complex (FFSC)

Fusarium infection, which can cause stem rot in sugarcane.

3.1.4. Fusarium oxysporum species complex (FOSC)

3.1.2. Fusarium proliferatum

controlling the disease.

Comparative genomics allows investigating many questions of evolutionary and functional significance of sequence features. By associating the species-specific genes with the unique characteristic of that species, researchers can find the potential relationship between genotype and phenotype. Various forward and reverse genetic methods have been developed to explore the repertoire of Fusarium genes contributing to disease formation, mycotoxin production, and sporulation. Phylogenetic tree is another application of comparative genomics to infer evolutionary relationship and to estimate diverge time based on the sequence similarity.

The whole genome of three fungal isolates (CNO1, YN41, and BS2–BS6) from the Fusarium species complex (FSC) that caused pokkah boeng disease of sugarcane was sequenced by Illumina and PacBio platforms. The genome coverages ranged from 100 to 200. The newly sequenced genomes, along with five previously sequenced isolates (F. fujikuroi IMI58289, F. verticillioides 7600, F. mangiferae, F. circinatum FSP34, and F. oxysporum 4287), were selected based on their incidence in geographical locations, taxonomy/species, host isolation, toxin production, and pathology. Overall, the eight sequenced genomes were comparable in size and structure. The sizes of the eight sequenced genomes ranged from 41.9 to 61.4 Mb with approximately 48.0 of GC content (from 47.3 to 48.3). The CDS (protein-coding genes) ranged from 10,522 to 17,753. The gene density ranged from 284 to 356 per Mb.

The development of genomics is allowing the incorporation of new tools and resources to address the important new challenges for agriculture. The commercial sugarcane cultivars used today resulted from crosses of S. officinarum and S. spontaneum. However, the reproductive biology and complex genome of sugarcane complicate breeding of genetically improved varieties by conventional means. The global relationship between the linear sequence of nucleic acid bases in the DNA of the gene and the sequence of amino acids in the protein encoded by the DNA of S. officinarum and S. spontaneum associated these chromosome exchanges occurred through recombinations between the chromosomes of the two species.

biosynthesis of the secondary metabolite, genes with regulatory and transport functions are

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The Fusarium comparative genomics highlighted the existence of lineage-specific chromosomes that are enriched for transposable elements and encoded genes that are pathogenicity related. These lineage-specific chromosomes play significant roles in adaptation to changing environments among this species complex. The well-defined genetic model system, will help to redefine the control strategies for pathogens with such genetic pathogenicity; qualitative genes are more often inherited dominantly and are also normally found clustered together in certain chromosome arms. These genes have major effects and are expressed throughout the life of a plant, tending to produce a plant completely resistant to one or more strains of a

Modern sugarcane cultivars were derived from the interspecific crosses among a few clones of S. officinarum, S. barberi, and S. sinense and the wild relatives of S. spontaneum and S. robustum. The wild species has played an important role in the development of adaptation or tolerance to varied abiotic and biotic stresses. After the initial interspecific crosses, sugarcane breeders concentrated their attention on intercrossing of the hybrid derivatives. The direct contribution of the chromosomes to pathogenicity is indicated by the fact that they encode known virulence factors such as effector proteins, necrosis-inducing peptides, and a large array of enzymes targeting plant substrates but lack genes involved in primary metabolism. Effectors are "secreted proteins and other molecules which allow plant-associated organisms to modulate plant defense circuitry and enable colonization of plant tissue" [22]. F. verticillioides, F. proliferatum, and several other species can produce a variety of mycotoxins (e.g., trichothecenes or fumonisins)

Nitrogen is one of the most important nutrients for crop growth and production. It is a major component in chlorophyll, which is the most important pigment needed for photosynthesis, as well as amino acids, the key building blocks of proteins. Nitrogen accelerates growth, gives vitality to plants, and promotes dark green color in leaves due to better chlorophyll synthesis. Sugarcane is the world's largest sugar crop and an economically important crop in China. The symptoms of sugarcane pokkah boeng tend to develop during periods in which high concen-

Fungi are able to respond to quantitative and qualitative changes in nitrogen availability through complex regulatory mechanisms. The source of nitrogen has been isolated from sugarcane (Saccharum spp.) as beneficial interaction that promotes plant growth. Nitrogen is the most essential factor having direct effect on cane growth, sugarcane yield, and juice quality. However, nitrogen application at high rates exceeding sugarcane plant utilization has adverse effect on cane quality. The beneficial plant microorganism association has unique features that remain to be characterized. This living microorganism, which has potential for the development of plant growth by civilizing the nutrient condition of the plant and inadequate supply of

usually also present in these clusters.

that are associated with plant disease.

trations of nitrogen are applied.

3.3. Fusarium species complex and nitrogen source

nitrogen, decreases the plant metabolism and growth.

particular pathogen.

A comparative genomics approach was effective in resolving the genetic relationship among fungal species and isolates. The Fusarium fujikuroi species complex (FFC) causes a wide spectrum of devastating diseases on diverse agricultural crops, like sugarcane. This is in part due to the complexity of the sugarcane genome which is probably the most complex of all plant crops. Sugarcane complex genome structure is due to a number of interesting developments that resulted in the sugarcane varieties grown.

FFC species can produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin and the phytohormones gibberellins, auxins, and cytokinins. Fusarium-induced crop diseases as well as mycotoxin contamination problems result in significant economic losses to world agriculture every year. The major discoveries contributed by genomic analyses of Fusarium focused on plant pathogenicity, production of mycotoxins, and other secondary metabolites. A key theme is the finding that a Fusarium genome is compartmentalized into core and adaptive regions that encode functions associated mostly with primary growth versus adaptation to specific niches (e.g., virulence on specific hosts, growth in specific environments). This genome compartmentalization should enable functional studies focused on the development of improved means for controlling Fusarium diseases and toxin contamination.

Secondary metabolites are very important in mediating interactions between fungus and host plant. The genes encoded the secondary metabolite often involve particular types of key enzymes, including polyketide synthase (PKS), non-ribosomal peptide synthetase (NPS), and terpenoid synthase. These key enzymes are clustered along with various combinations of additional enzymes for further metabolite catalyzing and with transporters and transcription factors that are essential for the regulation of most of the clustered genes. Based on the fungal SM analyses by antiSMASH software, some SM biosynthetic gene clusters were shared in all Fusarium species complex causing sugarcane pokkah boeng, including aflatrem, fusaric acid, fusarubin, asperfuranone, bikaverin, acetylaranotin, fusaridione A, equisetin, nivalenol/ deoxynivalenol/3-acetyldeoxynivalenol, and fujikurins. Ustilagic acid was only produced in F. oxysporum 4287. The gene clusters for azaphilone, fumonisin, and apicidin biosynthesis were only available in F. proliferatum. Gibberellin acid was only produced in F. verticillioides CNO1, but not in F. proliferatum YN41 because the genome of F. proliferatum YN41 lacked one of the key genes (p450) in GA biosynthetic gene cluster, which regulated GA production.

The genotype F. verticillioides isolates exposed the event of non-toxigenic strains and confirmed that their phenotype was likely the deletion of genes which are requisite for fumonisin biosynthesis. Some Fusarium secondary metabolite gene clusters exhibit a discontinuous distribution that does not correlate with phylogenetic relationships of species. For example, the intently distributed fumonisin and gibberellin gene clusters are present in some but not all species of the F. fujikuroi and F. oxysporum species complexes. Along with genes responsible for the biosynthesis of the secondary metabolite, genes with regulatory and transport functions are usually also present in these clusters.

The Fusarium comparative genomics highlighted the existence of lineage-specific chromosomes that are enriched for transposable elements and encoded genes that are pathogenicity related. These lineage-specific chromosomes play significant roles in adaptation to changing environments among this species complex. The well-defined genetic model system, will help to redefine the control strategies for pathogens with such genetic pathogenicity; qualitative genes are more often inherited dominantly and are also normally found clustered together in certain chromosome arms. These genes have major effects and are expressed throughout the life of a plant, tending to produce a plant completely resistant to one or more strains of a particular pathogen.

Modern sugarcane cultivars were derived from the interspecific crosses among a few clones of S. officinarum, S. barberi, and S. sinense and the wild relatives of S. spontaneum and S. robustum. The wild species has played an important role in the development of adaptation or tolerance to varied abiotic and biotic stresses. After the initial interspecific crosses, sugarcane breeders concentrated their attention on intercrossing of the hybrid derivatives. The direct contribution of the chromosomes to pathogenicity is indicated by the fact that they encode known virulence factors such as effector proteins, necrosis-inducing peptides, and a large array of enzymes targeting plant substrates but lack genes involved in primary metabolism. Effectors are "secreted proteins and other molecules which allow plant-associated organisms to modulate plant defense circuitry and enable colonization of plant tissue" [22]. F. verticillioides, F. proliferatum, and several other species can produce a variety of mycotoxins (e.g., trichothecenes or fumonisins) that are associated with plant disease.

#### 3.3. Fusarium species complex and nitrogen source

biology and complex genome of sugarcane complicate breeding of genetically improved varieties by conventional means. The global relationship between the linear sequence of nucleic acid bases in the DNA of the gene and the sequence of amino acids in the protein encoded by the DNA of S. officinarum and S. spontaneum associated these chromosome exchanges occurred

A comparative genomics approach was effective in resolving the genetic relationship among fungal species and isolates. The Fusarium fujikuroi species complex (FFC) causes a wide spectrum of devastating diseases on diverse agricultural crops, like sugarcane. This is in part due to the complexity of the sugarcane genome which is probably the most complex of all plant crops. Sugarcane complex genome structure is due to a number of interesting developments that

FFC species can produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin and the phytohormones gibberellins, auxins, and cytokinins. Fusarium-induced crop diseases as well as mycotoxin contamination problems result in significant economic losses to world agriculture every year. The major discoveries contributed by genomic analyses of Fusarium focused on plant pathogenicity, production of mycotoxins, and other secondary metabolites. A key theme is the finding that a Fusarium genome is compartmentalized into core and adaptive regions that encode functions associated mostly with primary growth versus adaptation to specific niches (e.g., virulence on specific hosts, growth in specific environments). This genome compartmentalization should enable functional studies focused on the development of improved means for

Secondary metabolites are very important in mediating interactions between fungus and host plant. The genes encoded the secondary metabolite often involve particular types of key enzymes, including polyketide synthase (PKS), non-ribosomal peptide synthetase (NPS), and terpenoid synthase. These key enzymes are clustered along with various combinations of additional enzymes for further metabolite catalyzing and with transporters and transcription factors that are essential for the regulation of most of the clustered genes. Based on the fungal SM analyses by antiSMASH software, some SM biosynthetic gene clusters were shared in all Fusarium species complex causing sugarcane pokkah boeng, including aflatrem, fusaric acid, fusarubin, asperfuranone, bikaverin, acetylaranotin, fusaridione A, equisetin, nivalenol/ deoxynivalenol/3-acetyldeoxynivalenol, and fujikurins. Ustilagic acid was only produced in F. oxysporum 4287. The gene clusters for azaphilone, fumonisin, and apicidin biosynthesis were only available in F. proliferatum. Gibberellin acid was only produced in F. verticillioides CNO1, but not in F. proliferatum YN41 because the genome of F. proliferatum YN41 lacked one of the

key genes (p450) in GA biosynthetic gene cluster, which regulated GA production.

The genotype F. verticillioides isolates exposed the event of non-toxigenic strains and confirmed that their phenotype was likely the deletion of genes which are requisite for fumonisin biosynthesis. Some Fusarium secondary metabolite gene clusters exhibit a discontinuous distribution that does not correlate with phylogenetic relationships of species. For example, the intently distributed fumonisin and gibberellin gene clusters are present in some but not all species of the F. fujikuroi and F. oxysporum species complexes. Along with genes responsible for the

through recombinations between the chromosomes of the two species.

146 Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

resulted in the sugarcane varieties grown.

controlling Fusarium diseases and toxin contamination.

Nitrogen is one of the most important nutrients for crop growth and production. It is a major component in chlorophyll, which is the most important pigment needed for photosynthesis, as well as amino acids, the key building blocks of proteins. Nitrogen accelerates growth, gives vitality to plants, and promotes dark green color in leaves due to better chlorophyll synthesis. Sugarcane is the world's largest sugar crop and an economically important crop in China. The symptoms of sugarcane pokkah boeng tend to develop during periods in which high concentrations of nitrogen are applied.

Fungi are able to respond to quantitative and qualitative changes in nitrogen availability through complex regulatory mechanisms. The source of nitrogen has been isolated from sugarcane (Saccharum spp.) as beneficial interaction that promotes plant growth. Nitrogen is the most essential factor having direct effect on cane growth, sugarcane yield, and juice quality. However, nitrogen application at high rates exceeding sugarcane plant utilization has adverse effect on cane quality. The beneficial plant microorganism association has unique features that remain to be characterized. This living microorganism, which has potential for the development of plant growth by civilizing the nutrient condition of the plant and inadequate supply of nitrogen, decreases the plant metabolism and growth.

Nitrogen availability has significant effects not only on physiological and morphological characteristics of the fungus but also on the biosynthesis of secondary metabolites, such as mycotoxins in Fusarium species complex. It is difficult to determine exact N requirements of sugarcane crop. The use of organic nitrogen base may reduce the disease outbreaks and improve antagonistic to pathogens on certain fungi and microorganisms.

The secondary metabolism, also called specialized metabolism, is part of the metabolism of fungi which is not essential for direct survival; such gene will rely on regulatory mechanisms for biosynthesis and their perpetual relations with the nitrogen regulation of other pathways in Fusarium, a paradigmatic model fungus for secondary metabolism. The screening of plant genotypes for their enhanced ability to acquire nitrogen by BNF can reduce the use of expensive nitrogen fertilizers in several important cash crops like sugarcane. In fact, the condition of biologically fixed nitrogen plays a key role in crop production in world cultivation. To understand the role of biological nitrogen fixation, more research work is needed for improving

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efficiency of nitrogen in order to reduce the use of synthetic fertilizer for production.

forms of fertilizers.

3.4. Preventive and control measures

incorporated approach is often recommended.

Fusarium species having plant growth-promoting activities are exploited for growing agricultural needs. The Fusarium spp. range in their pathogenicity, but they can produce mycotoxins in sugarcane. Such Fusarium includes the Gibberella fujikuroi species complex especially F. verticillioides and F. proliferatum, which can cause stalk root. Under alternating drought/wet conditions, F. verticillioides produces toxic fumonisins and under warm in certain crop variety. The effects of nitrogen sources on F. verticillioides will lead to pigmentation variation derived from bikaverin, fusarubins, and carotenoids. The gene expression in F. verticillioides was established to analyze processes modulated by different sources of nitrogen and to identify new regulatory mechanisms. Historically, a small amount of N fertilizers was recommended at planting to aid in early season fall growth as well as a mid-season N fertilization application in early spring. Excess N can lead to prolonged vegetative growth and reduced sucrose concentration mainly due to increased moisture in stalks. Crop response to immunization with symbiotic nitrogen has established their important role in supplementing nitrogen to the plant, allowing a sustainable use of nitrogen fertilizers. Today, nitrogen source of applications on sugarcane tends to be a complicated issue due to previous research showing contradicting results. However, economic factors and soil reaction must be considered while selecting the

Sugarcane is a highly industrious crop which suffers from numerous diseases caused by different organisms and factors such as environmental and physiological disorders and nutritional deficiencies. Historically, planting susceptible varieties in a large area encouraged the outbreak of a certain diseases in a particular period of time. Several control measures may be implemented to reduce potential sugarcane yield loss caused by pests and diseases, but an

Disease control in sugarcane is based on an integration of legislative control, resistant cultivars, and other management procedures. Short-term spraying options are available, but their economic viability may not be sustained. Machine harvest can also transmit disease. Many sugarcane diseases are also managed through the use of disease-free planting material supplied through Cane Protection and Productivity Boards. The genetical resistant cultivars is the most cost-effective method to control the disease, and the presence of genetic variations against pokkah boeng and its associates is well documented. Because of the more serious disease

On the other hand, it has been observed that in some plants as the N content is increased beyond sufficient levels, the amount of antifungal compounds decreases. Nitrogen fixation is a biological process that reduces molecular N2 into ammonia (NH3), which can be easily absorbed by plants. During this adaptation, nitrogenase plays a very important role in catalysis. Strains with nitrogenase activity were identified on the basis of their phenotypic and 16S rDNA sequence analysis and concluded that isolates had potential for regulation of plant growth. To synthesize the secondary metabolites of nitrogen molecules, ammonia plays the vital role in plant growth and development.

Nitrogen supply can bang plant-pathogen interactions through consequence on pathogen virulence. The well-established virulence factor of Fusarium oxysporum was found to repress the capacity of the fungal species to penetrate cellophane membrane through the nitrogen source. Nitrogen Utilization may be either regular or impartial with phosphorus and potassium so that nitrification can take place properly. Due to swift mobility of nitrogen, its effect is quite visible in the form of rapid growths due to its presence and rapid retardation in growth and of the crop due to its deficiency. The profuse of nitrogen can enhance the production of young, luscious growth, an expanded vegetative period, and tardy ripeness. The N requirements vary with climate, crop growth, cane yield pattern, irrigation frequency and distribution, land preparation, soil types, and soil behavior. The sustainability of prospective crops is strongly reliant on minimization of fertilizer inputs that can be achieved by enhancement of plant-associated nitrogen fixation. The various applications of nitrogen have been allied with increase in yield. Similarly, the heavy use of nitrogen can promote lodging which can reduce potential yield. In exacting, the nitrogen accessibility directly modulates the regulation of nitrogen source.

Biofertilizers are based on effective strains of microorganisms in sufficient numbers, which are useful for nitrogen fixation in plants and synthesis of growth-promoting substances like hormones, vitamins, and auxins. Besides being essential as a source of cheap protein for human nutrition and animal feed, symbiosis with rhizobia is essential in crop rotation to maintain soil fertility. Poultry manure and other animal waste products were used as a source of supplemental nitrogen long before inorganic nitrogen fertilizer came into popular use. The utilization of BNF for agricultural purposes has long been the dynamic force behind N-fixation research. The environmental benefits from using biological N-fixation are seen to be associated with the proxy of chemical-based technologies with a biological system. Some of the main benefits provided through crop rotation include the prevention of soil erosion, increased soil microorganism diversity, decreased pest prevalence, and increased field fertility. The importance of field fertility in the process of growing crop is immense. The process of BNF can be defined as the reduction of dinitrogen to ammonia by means of a prokaryote. BNF is accomplished by a wide variety of prokaryotes; some can accomplish this as free living organisms, while others require a symbiotic association with plants.

The secondary metabolism, also called specialized metabolism, is part of the metabolism of fungi which is not essential for direct survival; such gene will rely on regulatory mechanisms for biosynthesis and their perpetual relations with the nitrogen regulation of other pathways in Fusarium, a paradigmatic model fungus for secondary metabolism. The screening of plant genotypes for their enhanced ability to acquire nitrogen by BNF can reduce the use of expensive nitrogen fertilizers in several important cash crops like sugarcane. In fact, the condition of biologically fixed nitrogen plays a key role in crop production in world cultivation. To understand the role of biological nitrogen fixation, more research work is needed for improving efficiency of nitrogen in order to reduce the use of synthetic fertilizer for production.

Fusarium species having plant growth-promoting activities are exploited for growing agricultural needs. The Fusarium spp. range in their pathogenicity, but they can produce mycotoxins in sugarcane. Such Fusarium includes the Gibberella fujikuroi species complex especially F. verticillioides and F. proliferatum, which can cause stalk root. Under alternating drought/wet conditions, F. verticillioides produces toxic fumonisins and under warm in certain crop variety. The effects of nitrogen sources on F. verticillioides will lead to pigmentation variation derived from bikaverin, fusarubins, and carotenoids. The gene expression in F. verticillioides was established to analyze processes modulated by different sources of nitrogen and to identify new regulatory mechanisms. Historically, a small amount of N fertilizers was recommended at planting to aid in early season fall growth as well as a mid-season N fertilization application in early spring. Excess N can lead to prolonged vegetative growth and reduced sucrose concentration mainly due to increased moisture in stalks. Crop response to immunization with symbiotic nitrogen has established their important role in supplementing nitrogen to the plant, allowing a sustainable use of nitrogen fertilizers. Today, nitrogen source of applications on sugarcane tends to be a complicated issue due to previous research showing contradicting results. However, economic factors and soil reaction must be considered while selecting the forms of fertilizers.

#### 3.4. Preventive and control measures

Nitrogen availability has significant effects not only on physiological and morphological characteristics of the fungus but also on the biosynthesis of secondary metabolites, such as mycotoxins in Fusarium species complex. It is difficult to determine exact N requirements of sugarcane crop. The use of organic nitrogen base may reduce the disease outbreaks and

On the other hand, it has been observed that in some plants as the N content is increased beyond sufficient levels, the amount of antifungal compounds decreases. Nitrogen fixation is a biological process that reduces molecular N2 into ammonia (NH3), which can be easily absorbed by plants. During this adaptation, nitrogenase plays a very important role in catalysis. Strains with nitrogenase activity were identified on the basis of their phenotypic and 16S rDNA sequence analysis and concluded that isolates had potential for regulation of plant growth. To synthesize the secondary metabolites of nitrogen molecules, ammonia plays the

Nitrogen supply can bang plant-pathogen interactions through consequence on pathogen virulence. The well-established virulence factor of Fusarium oxysporum was found to repress the capacity of the fungal species to penetrate cellophane membrane through the nitrogen source. Nitrogen Utilization may be either regular or impartial with phosphorus and potassium so that nitrification can take place properly. Due to swift mobility of nitrogen, its effect is quite visible in the form of rapid growths due to its presence and rapid retardation in growth and of the crop due to its deficiency. The profuse of nitrogen can enhance the production of young, luscious growth, an expanded vegetative period, and tardy ripeness. The N requirements vary with climate, crop growth, cane yield pattern, irrigation frequency and distribution, land preparation, soil types, and soil behavior. The sustainability of prospective crops is strongly reliant on minimization of fertilizer inputs that can be achieved by enhancement of plant-associated nitrogen fixation. The various applications of nitrogen have been allied with increase in yield. Similarly, the heavy use of nitrogen can promote lodging which can reduce potential yield. In

exacting, the nitrogen accessibility directly modulates the regulation of nitrogen source.

while others require a symbiotic association with plants.

Biofertilizers are based on effective strains of microorganisms in sufficient numbers, which are useful for nitrogen fixation in plants and synthesis of growth-promoting substances like hormones, vitamins, and auxins. Besides being essential as a source of cheap protein for human nutrition and animal feed, symbiosis with rhizobia is essential in crop rotation to maintain soil fertility. Poultry manure and other animal waste products were used as a source of supplemental nitrogen long before inorganic nitrogen fertilizer came into popular use. The utilization of BNF for agricultural purposes has long been the dynamic force behind N-fixation research. The environmental benefits from using biological N-fixation are seen to be associated with the proxy of chemical-based technologies with a biological system. Some of the main benefits provided through crop rotation include the prevention of soil erosion, increased soil microorganism diversity, decreased pest prevalence, and increased field fertility. The importance of field fertility in the process of growing crop is immense. The process of BNF can be defined as the reduction of dinitrogen to ammonia by means of a prokaryote. BNF is accomplished by a wide variety of prokaryotes; some can accomplish this as free living organisms,

improve antagonistic to pathogens on certain fungi and microorganisms.

148 Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

vital role in plant growth and development.

Sugarcane is a highly industrious crop which suffers from numerous diseases caused by different organisms and factors such as environmental and physiological disorders and nutritional deficiencies. Historically, planting susceptible varieties in a large area encouraged the outbreak of a certain diseases in a particular period of time. Several control measures may be implemented to reduce potential sugarcane yield loss caused by pests and diseases, but an incorporated approach is often recommended.

Disease control in sugarcane is based on an integration of legislative control, resistant cultivars, and other management procedures. Short-term spraying options are available, but their economic viability may not be sustained. Machine harvest can also transmit disease. Many sugarcane diseases are also managed through the use of disease-free planting material supplied through Cane Protection and Productivity Boards. The genetical resistant cultivars is the most cost-effective method to control the disease, and the presence of genetic variations against pokkah boeng and its associates is well documented. Because of the more serious disease problem, a progressive effort to socialize and conduct integrated management for controlling the disease.

Several control measures may be implemented to reduce potential sugarcane yield loss caused by pests and diseases, but an incorporated approach is often recommended. To remove and destruct of infected plants on the first appearance of the disease in case of pokkah boeng established that frequent breakdown of varietal resistance against pokkah boeng is due to the appearance of new pathotypes matching the resistance of cane genotypes.

Successive ratoons are characterized by reductions in cane yield due to systemic diseases or physical damage to stools, and the number of ratoons obtained from a single harvest also depends on genotypic and environmental factors. Ratoon productivity has been proved to increase with proper management involving timely agricultural operations, proper nutrition management and integrated pest management, and maintenance of adequate plant population. A number of ratoon management practices currently in use, such as inter-row ripping, burning of crop residues at harvest, harvesting under wet conditions, and using heavy infield transport, were found to be contrary with the substantial, chemical, and biological properties of the soil. The incidence was figured out as five grades (Figure 1).

Based on the disease severity index (DSI) of pokkah boeng disease of sugarcane, the resistance of sugarcane against pokkah boeng was classified into five levels from 0 to 5. Level 0 was defined as highly resistant (HR) with DSI≦1.0, Level 1 as resistant (R) with DSI ranged from 1.1 to 5.0, Level 2 as moderately resistant (MR) with DSI from 5.1 to 10.0, Level 3 as moderately susceptible (MS) with DSI from 10.1to 15.0, Level 4 as susceptible (S) with DSI from 15.1 to 20.0, and Level 5 as highly susceptible (HS) with DSI > 20.0.

The disease severity index (DSI) was calculated as follows:

$$\text{DSI} = \left[ \frac{\text{Sum of all numerical grades}}{(\text{Total number of plants counted} \times \text{maximum grade})} \right] \times 100\tag{1}$$

Conidial suspensions of the isolates (CNO-1 and YN41, 106 conidia mL�<sup>1</sup> , 100 μL) were dripped into the young spindle of 89 sugarcane germplasm, and the symptoms were observed on the inoculated plants in 6–8 days post-inoculation, respectively. Our results showed that 34 of 89 tested clones (38.2%) were susceptible to both CNO1 and YN41, 32 clones (36.0%) susceptible to CNO-1 but resistant to YN-41, 14 clones (15.7%) susceptible to YN41 but resistant to CNO-1, and only 8 clones (9.0%) resistant to both CNO-1 and resistant YN41. Both these resistant clones included CP84-1198, GT94-40, GT05-3846, ROC1, ROC27, YC58-14, YC64-173, and YT94-128. Moreover, our results also showed that CNO-1 had higher infection than YN-41 by this inoculation with a success of up to 89.8%.

Chemical control is often expensive and has downstream unconstructive effects on the environment. Nine compounds were tested at three concentrations (100, 50, and 10 ppm) for their ability to inhibit mycelial growth of Fusarium species complex. Two antibacterial compounds including copper 8-hydroxyquinoline and validamycins had no effect on the mycelial growth of Fusarium species complex at 10 ppm and partially inhibited mycelial growth at the concentration of 50 and 100 ppm. In addition, two compounds, including thifluzamide and chloroisobromine

Figure 1. Symptoms of sugarcane pokkah boeng disease in China.

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problem, a progressive effort to socialize and conduct integrated management for controlling

Several control measures may be implemented to reduce potential sugarcane yield loss caused by pests and diseases, but an incorporated approach is often recommended. To remove and destruct of infected plants on the first appearance of the disease in case of pokkah boeng established that frequent breakdown of varietal resistance against pokkah boeng is due to the

Successive ratoons are characterized by reductions in cane yield due to systemic diseases or physical damage to stools, and the number of ratoons obtained from a single harvest also depends on genotypic and environmental factors. Ratoon productivity has been proved to increase with proper management involving timely agricultural operations, proper nutrition management and integrated pest management, and maintenance of adequate plant population. A number of ratoon management practices currently in use, such as inter-row ripping, burning of crop residues at harvest, harvesting under wet conditions, and using heavy infield transport, were found to be contrary with the substantial, chemical, and biological properties

Based on the disease severity index (DSI) of pokkah boeng disease of sugarcane, the resistance of sugarcane against pokkah boeng was classified into five levels from 0 to 5. Level 0 was defined as highly resistant (HR) with DSI≦1.0, Level 1 as resistant (R) with DSI ranged from 1.1 to 5.0, Level 2 as moderately resistant (MR) with DSI from 5.1 to 10.0, Level 3 as moderately susceptible (MS) with DSI from 10.1to 15.0, Level 4 as susceptible (S) with DSI from 15.1 to

> ð Þ Total number of plants counted � maximum grade

into the young spindle of 89 sugarcane germplasm, and the symptoms were observed on the inoculated plants in 6–8 days post-inoculation, respectively. Our results showed that 34 of 89 tested clones (38.2%) were susceptible to both CNO1 and YN41, 32 clones (36.0%) susceptible to CNO-1 but resistant to YN-41, 14 clones (15.7%) susceptible to YN41 but resistant to CNO-1, and only 8 clones (9.0%) resistant to both CNO-1 and resistant YN41. Both these resistant clones included CP84-1198, GT94-40, GT05-3846, ROC1, ROC27, YC58-14, YC64-173, and YT94-128. Moreover, our results also showed that CNO-1 had higher infection than YN-41 by this inocula-

Chemical control is often expensive and has downstream unconstructive effects on the environment. Nine compounds were tested at three concentrations (100, 50, and 10 ppm) for their ability to inhibit mycelial growth of Fusarium species complex. Two antibacterial compounds including copper 8-hydroxyquinoline and validamycins had no effect on the mycelial growth of Fusarium species complex at 10 ppm and partially inhibited mycelial growth at the concentration of 50 and 100 ppm. In addition, two compounds, including thifluzamide and chloroisobromine

� 100 (1)

, 100 μL) were dripped

appearance of new pathotypes matching the resistance of cane genotypes.

150 Fusarium - Plant Diseases, Pathogen Diversity, Genetic Diversity, Resistance and Molecular Markers

of the soil. The incidence was figured out as five grades (Figure 1).

20.0, and Level 5 as highly susceptible (HS) with DSI > 20.0.

The disease severity index (DSI) was calculated as follows:

tion with a success of up to 89.8%.

DSI <sup>¼</sup> Sum of all numerical grades

Conidial suspensions of the isolates (CNO-1 and YN41, 106 conidia mL�<sup>1</sup>

the disease.

Figure 1. Symptoms of sugarcane pokkah boeng disease in China.

cyanuric acid, had great effect on inhibition of fungal growth at 10 ppm rather than at 50 and 100 ppm.

[5] Bryden WL, Logrieco A, Abbas HK, Porter JK, Vesonder RF, Richard JL, Cole RJ. Other significant Fusarium mycotoxins. In: Summerell BA, Leslie JF, Backhouse D, Bryden WL, Burgess LW, editors. Fusarium: Paul E. Nelson Memorial Symposium. St. Paul, MN: APS

*Fusarium* Species Complex Causing Pokkah Boeng in China

http://dx.doi.org/10.5772/intechopen.73133

153

[6] Logrieco A, Doko MB, Moretti A, Frisullo S, Visconti A. Occurrence of FB1 and FB2 in Fusarium proliferatum infected asparagus plants. Journal of Agricultural and Food Chem-

[7] Gupta S, Krasnoff SB, Underwood NL, Renwick JAA, Roberts DW. Isolation of beauvericin as an insect toxin from Fusarium semitectum and Fusarium moniliforme var. Subglu-

[8] Logrieco A, Moretti A, Fornelli F, Fogliano V, Ritieni A, Caiaffa MF, Randazzo G, Bottalico A, Macchia L. Fusaproliferin production by Fusarium subglutinans and its toxicity to Artemia Salina, SF-9 insect cells, and IARC/LCL 171 human B lymphocytes.

[9] Geh SL. Current status of diseases and pests of sugarcane in West Malaysia. 1973. pp. 4-6.

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In the field test, spraying of different fungicides like Bavistin or Blitox or copper oxychloride or carbendazim is efficient for reducing the pokkah boeng disease. Planting of healthy seed, the use of resistant varieties, and following the integrated disease management practices are the best ways to prevent disease incidence. The use of resistant cultivars is particularly useful, as it reduces the use of harmful chemicals which can disturb the balance of nature and result in other pests becoming a problem. Furthermore, Fusarium spp. prevalence in the soil can be affected considerably by crop rotation practices. Although the use of resistant varieties is the best means of control, some strains have been found to overcome resistance, and the once-resistant varieties were reported to be susceptible.

Host plant resistance shows major advantages compared to chemical, biological, and cultural control components for management programmes. However, it needs to be supported with additional management practices to ensure durability in the field. Biological control of plant pathogens is an attractive alternative to the strong dependence of modern agriculture on chemical fungicides, which cause environmental pollution and development of resistant strains. The endophytic bacterial community associated with sugarcane harbors multiple genera with potential for plant growth promotion and disease control.
