**A Practical Way to ISO/GUM Measurement Uncertainty for Analytical Assays Including In-House Validation Data** A Practical Way to ISO/GUM Measurement Uncertainty for Analytical Assays Including In-House Validation Data

DOI: 10.5772/intechopen.72048

Antonio Gustavo González, María Ángeles Herrador, Agustín García Asuero and Julia Martín Antonio Gustavo González, María Ángeles Herrador, Agustín García Asuero and Julia Martín

Additional information is available at the end of the chapter Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.72048

#### Abstract

In this contribution, we outline the estimation of measurement uncertainty of analytical assays in a practical way, according to the so-called reconciliation paradigm, by considering the heritance of uncertainties according to the ISO Guide to the expression of uncertainty in measurement (ISO/GUM) approach and the accuracy (bias and precision) study coming from the in-house method validation. A cause and effect analysis is performed by using the Ishikawa diagram or fishbone plot, consisting of a hierarchical structure reaching a final outcome that is the analytical result. The procedure is illustrated with a case study. This procedure may be very suitable for processing data in accreditation of routine assays.

Keywords: ISO/GUM approach, method validation, uncertainty measurement
