*3.3.2.2. Molecular techniques*

Molecular techniques for prenatal diagnosis of chromosomal disorders were introduced in the medical practice in the last 25 years with the aim of improving the resolution of chromosomal detection and to eliminate the major inconvenient of classic cytogenetic techniques the requirement of cell culture. Such techniques are fluorescence in situ hybridization (FISH), quantitative fluorescent polymerase chain reaction (QF-PCR), multiplex ligation probe amplification (MLPA) and array-comparative genome hybridization (CHG).
