**Acknowledgements**

**Figure 18.** Influence of the pulsed voltage stimulation on hypha growth in agar medium cultivation. The diameter of the petri dishes is 10 cm in the all cases. The inner and outer dotted circles indicate growth positions of hyphae at 5- and

**Figure 17.** Microscopic images of *Lentinula edodes* hypha (a) before and (b) after applying 5 kV/cm pulse electric field with pulse width of 100 ns and 500 times of repetition. (c) Superimposed image of two images (a) and (b). (d) and (e): SEM images of *L. edodes* hyphae before (d) and after (e) applying 10 kV pulse voltages. White bar indicates 100 μm in (a),

10-days cultivation, respectively [3].

(b), (c) and 10 μm in (d) and (e).

110 Physical Methods for Stimulation of Plant and Mushroom Development

The authors of this chapter confirm that they have received permission to reuse all the tables and figures in their current work.

## **Author details**

Koichi Takaki1 \*, Katsuyuki Takahashi1 and Yuichi Sakamoto2

\*Address all correspondence to: takaki@iwate-u.ac.jp


### **References**


[12] Idei T, Yoshizawa N, Tako M. Effects of electric shocks to the bed-logs of Lentinus edodes on fruiting-body production (in Japanese). Bulletin of the Utsunomiya University

High-Voltage Methods for Mushroom Fruit-Body Developments

http://dx.doi.org/10.5772/intechopen.79159

113

[14] Takaki K, Yamaguchi R, Kusaka T, Kofujita H, Takahashi K, Sakamoto Y, Narimatsu M, Nagane K. Effects of pulse voltage stimulation on fruit body formation in *Lentinula Edodes* cultivation. International Journal of Plasma Environmental Science and Technology.

[15] van Wetter M, Schuren F, Schuurs T, Wessels J. Targeted mutation of the SC3 hydrophobin gene of affects formation of aerial hyphae. FEMS Microbiology Letters. 1996;**140**:265-

[16] Wessels JGH. Gene expression during fruiting in Schizophyllum commune. Mycological

[13] Bluhm H. Pulsed Power Systems. Berlin, Germany: Springer; 2006

Forests. 1988;**24**:23-38

2010;**4**:109-113

269. DOI: 10.1016/0378-1097(96)00192-9

Research. 1992;**96**:609-620


**Author details**

1 Iwate University, Japan

Station. 1987;**33**:1-33

2001;**9**:7-12

2001;**6**:37-41

organisms. 2014;**2**:58-72

DOI: 10.1016/j.fbr.2018.02.003

\*, Katsuyuki Takahashi1

112 Physical Methods for Stimulation of Plant and Mushroom Development

\*Address all correspondence to: takaki@iwate-u.ac.jp

[1] Akiyama H, Heller R. Bioelectrics. Tokyo, Japan: Springer; 2017

2 Iwate Biotechnology Research Center, Japan

and Yuichi Sakamoto2

[2] Kaneko S, Yamamoto M, Nakashima Y, Jitsufuji Y. Studies on electrical stimulation on *Lentinula edodes* bed log (in Japanese). The Bulletin of Fukuoka-Ken Forest Experiment

[3] Takaki K, Yoshida K, Saito T, Kusaka T, Yamaguchi R, Takahashi K, Sakamoto Y. Effect of electrical stimulation on fruit body formation in cultivating mushrooms. Micro-

[4] Sakamoto Y. Influences of environmental factors on fruiting body induction, development and maturation in mushroom-forming fungi. Fungal Biology Reviews. 2018.

[5] Kudo S, Mitobe S, Yoshimura Y. Electric stimulation multiplication of *Lentinulus edodes*

[6] Ohga S, Iida S, Koo C-D, Cho N-S. Effect of electric impulse on fruit body production on *Lentinula edodes* in the sawdust-based substrate. Mushroom Science and Biotechnology.

[7] Ohga S, Cho N-S, Li Y, Royse DJ. Utilization of pulsed power to stimulate fructification

[8] Takaki K, Kanesawa K, Yamazaki N, Mukaigawa S, Fujiwara T, Takahasi K, Yamasita K, Nagane K. Effect of pulsed high-voltage stimulation on *Pholiota nameko* mushroom yield.

[9] Ohga S, Iida S. Effect of electric impulse on sporocarp formation of ectomycorrhizal fungus *Laccaria laccata* in Japanese red pine plantation. Journal of Forest Research.

[10] Islam F, Ohga S. The response of fruit body formation on Tricholoma matsutake in situ condition by applying electric pulse stimulator. ISRN Agronomy. 2012;**462724**:1-6 [11] Takaki K, Kanesawa K, Mukaigawa S, Fujiwara T, Go T. Energy efficiency of corona discharge reactor driven by inductive energy storage system pulsed power generator.

IEEE Transactions on Dielectrics and Electrical Insulation. 2007;**14**:834-845

(in Japanese). Journal of Institute of Electrostatics Japan. 1999;**23**:186-190

of edible mushroom. Mushroom Science. 2004;**16**:343-352

Acta Physica Polonica A. 2004;**115**:953-956

Koichi Takaki1

**References**

