**3.1 Microbial strain**

Potato dextrose agar (PDA) medium normally used by the scientist to maintained fungal isolates and consist of extract of boiled potatoes, 200 mL; dextrose, 20 g; agar, 20 g; deionized water, 800 mL at 28 C. Spore suspensions can be prepared and diluted in sterile potato dextrose broth (PDB) to a concentration of 107 spores per mL. The spore population needed can be counted using a haemocytometer. Subsequent dilutions can be made from the aforementioned suspension to adjust to the required concentration, which can be used in the antifungal test.
