**5. Acknowledgment**

Authors are grateful to Professor Mariana Sottomayor from IBMC- Institute for Molecular and Cell Biology for providing seeds for *in vitro* establishment of *Catharanthus* roseus. The authors also like to thank to Carina Alves, Luís Silva, Sandra Cabo and Tatiana Louçano, students of University of Trás-os-Montes and Alto Douro.
