**3.2 Screening for the antifungal effect**

Screening for antifungal effect can be carried out by using the disc diffusion method. The plate containing 25 mL of PDA medium will be seeded with 1 mL of fungal conidial spore suspension containing 105 spores per mL from a 120-h-old culture. Three Whatman filter paper No. 1 discs of 6-mm diameter can be used to screen the antifungal activity. Each sterile disk will be impregnated with 20 mL of the extract corresponding with 100 mg/mL of crude extract, myconazole 30 g/mL, as positive control, or vehicle as negative control. The plates will be refrigerated for 2 h to allow the compounds presents in the extract diffused and then will be incubated at 28 C for 5 days. Diameter of the inhibition zone will be measured, and the mean of the three replicates are taken (Bauer et al., 1966). The disc diffusion method is a qualitative test which could provide the information whether the crude extract possessed antifungal properties
