2.3. Induction of diabetes

The animals were fasted overnight and diabetes was induced by a single intraperitoneal injection of freshly prepared 150 mg/kg b.w alloxan monohydrate dissolved in (5%) sterile saline. Two days after alloxan injection, rats with blood glucose level of >12 mmol/L were separated and considered diabetic and were used for the study. Blood glucose levels were measured using blood glucose test strips with fine test glucometer (infopia Co. limited Korea). The treatment started 48 hours after alloxan injection and this was considered the first day of treatment. The treatment continued for 14 days.
