**7. Conclusions**

**Authors (year)**

Mangindaan et al. [100]

Delalat et al. [104]

Liu et al. [105]

**Plasma reactor/ monomers used**

84 Recent Research in Polymerization

RF reactor/ Aam

RF reactor/ Aam-OD

RF reactor/ Aam-OD

**Gradient formation method**

Use of a mask with a 1 mm gap on a polypropylene substrate

Moving slot with a simultaneous change in the monomer mixture composition

Moving slot with a simultaneous change in the monomer mixture composition

**Surface chemical properties**



Gradient over 12 mm of nitrogen content from 0 to 12.0%


**Table 2.** Overview of literature on amine gradient obtained by plasma polymerization and not discussed in the text.

**Biological assay/ cell type**

L-929 fibroblast culture

Mouse embryoid body cell culture


(FN)


**Bioresponsive properties**

Continuous increase in the cellular density with more than 2-fold density on








Aam sides.

Aam side

of serum

Aam side

Aam side.

N-rich end

From the work presented in this chapter it is clear that plasma polymer coatings are very useful tools for biomaterial surface modification. However, despite the numerous advantages of these coatings for biomaterial advancements, their aging and stability remain an issue that requires further investigations and considerations. More attention and focus on these aspects can make plasma polymerization become one of the most used and important surface modification techniques. Plasma polymer gradients are also very promising for biological applications and many advances in the area of plasma uses can be made by developing such coatings.
