**Transcriptome (ESTs) of Avocado "Native" Mexicano Early Seed Development Shows Abundance of Regulatory, Antioxidant and Defense Genes Early Seed Development Shows Abundance of Regulatory, Antioxidant and Defense Genes**

**Transcriptome (ESTs) of Avocado "Native" Mexicano** 

DOI: 10.5772/intechopen.70573

Luis María Suárez-Rodríguez, Fernando Sánchez-Albarrán, Huber León-Corona and Rodolfo López-Gómez Huber León-Corona and Rodolfo López-Gómez

Additional information is available at the end of the chapter Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.70573

Luis María Suárez-Rodríguez, Fernando Sánchez-Albarrán,

#### **Abstract**

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10.1186/1471-2164-12-190

44 Advances in Seed Biology

10.1186/1471-2164-11-730

10.3389/fpls.2017.00112

DOl: 10.1371/journal.pone.0137168

2010;**51**:795-809. DOl: 10.1093/pcp/pcq034

Avocado native "Mexicano" (*Persea americana* var drymifolia) has been a really important species in agricultural and indigenous medicine. In the agricultural world, it has been the germplasm source for the generation of economically important cultivars like Hass and it is the main source of rootstocks for the world production of Hass avocado fruit. In spite of its importance, little is known about the molecular network of seed-fruit development. The aim of this work was to know the expressed genes (ESTs) during the early avocado native "Mexicano" seed development. Using total RNA we constructed cDNA libraries of fourth months seed development, sequencing, assembling and bioinformatic analysis was made. For validation, a semi-quantitative PCR experiments with the most abundant genes were made. About 5005 ESTs from the 5' representing 1653 possible unigenes were isolated. After assembling process, we have 171 genes that are closely related to *Nelumbo nucifera* sequences. The transcriptome is dominating by one bHLH transcription factor, three metallothioneins and snakin, suggesting its main role in seed development. Until now, there are no molecular studies in avocado seed development.

**Keywords:** avocado fruit, transcriptome, transcription factor, stress, antimicrobials, cotyledon, alignment, domain, phylogenetic, metal homeostasis, cysteine, seed development, gene expression

© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
