**2. Materials and methods**

#### **2.1. Biological material**

Seeds from avocado nativo mexicano fruits of three stages of development (1, 4 and 8 months) were excised from fruits and frozen immediately in liquid nitrogen until use. The materials were collected in the avocado Germplasm Bank of the Instituto Nacional de Investigaciones Forestales y Agropecuarias (INIFAP; Uruapan, Michoacán México).
