**2.3. Assembly and identification of full-length cDNA**

Sanger sequences were assembled using default parameters of the 454 Newbler-Assembler v1.1.03.24 (454 Life Science, Branford, CT) using 16,526 generated by the University of Florida and the Washington University Genome Sequencing Center.

Unigene set was generated by combining all assembled contigs and non-assembled reads (singlets). The consensus sequences of the Unigenes were analyzed with EuGeneHom [23] to identify the Unigenes that contained the components of a complete cDNA (5′ UTR, ORF and 3′ UTR): http://genoweb.toulouse.inra.fr/eugene/EuGeneHom/cgi-bin/EuGeneHom.pl.
