5. Conclusion

The morphology of spermatozoa has a direct influence on the fertilization and developmental competence of embryos. A normal semen sample must have >30% of morphologically normal spermatozoa. Considering on the morphological study of patients spermatozoa, researchers, practicians and WHO have defined the prognosis of IVF treatment. Excellent prognosis is expected when >14% of normal spermatozoa were found in the semen sample. When 4–14% of normal spermatozoa were found, the IVF prognosis is good. When prognosis is poor (<4% of normal spermatozoa were found in the semen), the ICSI should be recommended for these patients [3, 18, 74–76].

[5] van der Horst G, Maree L. SpermBlue: A new universal stain for human and animal sperm which is also amenable to automated sperm morphology analysis. Biotechnic &

Assessment of Human Sperm Cells Morphological Parameters

http://dx.doi.org/10.5772/intechopen.71413

63

[6] Rapid SpermBlue® Protocol [Internet]. Available from: http://www.micropticsl.com/

[7] Auger J. Assessing human sperm morphology: Top models, underdogs or biometrics?

[8] Bellastella G, Cooper TG, Battaglia M, Ströse A, Torres I, Hellenkemper B, Soler C, Sinisi AA. Dimensions of human ejaculated spermatozoa in Papanicolaou-stained seminal and swim-up smears obtained from the Integrated Semen Analysis System (ISAS(®)). Asian

[9] Blanchard M, Haguenoer K, Apert A, Poret H, Barthélémy C, Royère D, Guérif F. Sperm morphology assessment using David's classification: Time to switch to strict criteria? Prospective comparative analysis in a selected IVF population. International Journal of

[10] Butts IA, Ward MA, Litvak MK, Pitcher TE, Alavi SM, Trippel EA, Rideout RM. Automated sperm head morphology analyzer for open-source software. Theriogenology.

[11] Soler C, De Monserrat JJ, Gutierrez R, Nunez J, Nunez M, Sancho M, Perez-Sanchez F, Cooper TG. Use of the sperm-class analyser for objective assessment of human sperm morphology. International Journal of Andrology. 2003;26:262-270. DOI: 10.1046/j.1365-

[12] MacLeod J. Human seminal cytology as a sensitive indicator of the germinal epithelium.

[13] Jouannet P, Ducot B, Feneux D, Spira A. Male factors and the likelihood of pregnancy in infertile couples. I. Study of sperm characteristics. International Journal of Andrology.

[14] Auger J, Eustache F, David G. Standardisation de la classification morphologique des spermatozoïdes humains selon la méthode de David modifiée. Andrologie. 2000;10:358-

[15] Hofmann N, Freundl G, Florack M, Die Formstorungen d. Spermatozoen im sperma und zervikalschleim als spiegel testikularer erkrankungen. Der Gynäkologe. 1985;18:189-192

[16] Hofmann N, Hilscher B. Use of aniline blue to assess chromatin condensation in morphologically normal spermatozoa in normal and infertile men. Human Reproduction.

[17] Kruger TF, Ackerman SB, Simmons KF, Swanson RJ, Brugo SS, Acosta AA. A quick, reliable staining technique for human sperm morphology. Archives of Andrology.

Histochemistry 2009;84:299-308. DOI: 10.3109/10520290902984274

Asian Journal of Andrology. 2010;12:36-46. DOI: 10.1038/aja.2009.8

Journal of Andrology. 2010;12:871-879. DOI: 10.1038/aja.2010.90

Andrology. 2011;34:145-152. DOI: 10.1111/j.1365-2605.2010.01066.x

2011;76:1756-1761. DOI: 10.1016/j.theriogenology.2011.06.019

International Journal of Fertility. 1964;9:281-295

1988;11:379-394. DOI: 10.1111/j.1365-2605.1988.tb01011.x

1991;6:979-982. DOI: 10.1093/oxfordjournals.humrep.a137472

1987;18:275-277. DOI: 10.3109/01485018708988493

2605.2003.00422.x

373. DOI: 10.1007/BF03034491

products/disposables/morphology/spermblue/

Therefore the analysis of the morphological parameters of spermatozoa using the light microscopy, MSOME, in combination with precise head birefringence detection using the polarized microscopy, could give the best fertilization rate and embryo quality after IVF and ICSI.
