**Author details**

Vlad-Mihai Voiculescu1,2, Constantin Caruntu<sup>1</sup> \*, Iulia Solomon2 , Mihai Lupu<sup>3</sup> , Mihaela Adriana Ilie<sup>1</sup> , Daniel Boda1,4, Carolina Constantin5,6 and Monica Neagu5,6,7

\*Address all correspondence to: costin.caruntu@gmail.com


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**Chapter 7**

. In

, over which an occlu-

**Provisional chapter**

**Correlation between Porcine and Human Skin Models**

**Correlation between Porcine and Human Skin Models** 

*Background:* Topical photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) as precursors of protoporphyrin IX (PPIX) have been used in skin cancer treatment and other skin diseases. To establish new topical PDT, protocols are necessary first to conduct studies *in vivo* using animal skin models. The goal of this study is to evaluate the robust correlation between porcine and human skin models *in vivo* by optical methods to confirm the suitability of porcine skin models to predict drug behavior in the human skin on topical PDT protocols. Methods: The study was performed *in vivo* using porcine and human skin models. In human skin, ALA and MAL cream mixture samples were applied to the inner arm in a circular area of 1 cm2

sive dressing was placed. PPIX production was monitored for up to 5 h using widefield fluorescence imaging and fluorescence spectroscopy techniques. Results: Human skin models showed similar behavior to porcine skin models, which indicates high similarity between both models and confirms that porcine skin is an adequate model to establish

**Keywords:** 5-ALA, MAL, porcine skin, human skin, widefield fluorescence imaging,

porcine skin, the cream was applied on the back in an area of 4 cm2

fluorescence spectroscopy, photodynamic therapy, skin models

new clinical PDT protocols in human volunteers.

© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

© 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use,

distribution, and reproduction in any medium, provided the original work is properly cited.

DOI: 10.5772/intechopen.75788

**by Optical Methods**

**by Optical Methods**

Alessandra Keiko Lima Fujita,

Alessandra Keiko Lima Fujita, Rozana Wendler da Rocha,

Vanderlei Salvador Bagnato and

Vanderlei Salvador Bagnato and

http://dx.doi.org/10.5772/intechopen.75788

Priscila Fernanda Campos de Menezes

Priscila Fernanda Campos de Menezes

Andrigo Barboza de Nardi,

**Abstract**

Rozana Wendler da Rocha, André Escobar,

André Escobar, Andrigo Barboza de Nardi,

Additional information is available at the end of the chapter

Additional information is available at the end of the chapter

#### **Correlation between Porcine and Human Skin Models by Optical Methods Correlation between Porcine and Human Skin Models by Optical Methods**

DOI: 10.5772/intechopen.75788

Alessandra Keiko Lima Fujita, Rozana Wendler da Rocha, André Escobar, Andrigo Barboza de Nardi, Vanderlei Salvador Bagnato and Priscila Fernanda Campos de Menezes Alessandra Keiko Lima Fujita, Rozana Wendler da Rocha, André Escobar, Andrigo Barboza de Nardi, Vanderlei Salvador Bagnato and Priscila Fernanda Campos de Menezes

Additional information is available at the end of the chapter Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.75788

#### **Abstract**

*Background:* Topical photodynamic therapy (PDT) using 5-aminolevulinic acid (ALA) and methyl aminolevulinate (MAL) as precursors of protoporphyrin IX (PPIX) have been used in skin cancer treatment and other skin diseases. To establish new topical PDT, protocols are necessary first to conduct studies *in vivo* using animal skin models. The goal of this study is to evaluate the robust correlation between porcine and human skin models *in vivo* by optical methods to confirm the suitability of porcine skin models to predict drug behavior in the human skin on topical PDT protocols. Methods: The study was performed *in vivo* using porcine and human skin models. In human skin, ALA and MAL cream mixture samples were applied to the inner arm in a circular area of 1 cm2 . In porcine skin, the cream was applied on the back in an area of 4 cm2 , over which an occlusive dressing was placed. PPIX production was monitored for up to 5 h using widefield fluorescence imaging and fluorescence spectroscopy techniques. Results: Human skin models showed similar behavior to porcine skin models, which indicates high similarity between both models and confirms that porcine skin is an adequate model to establish new clinical PDT protocols in human volunteers.

**Keywords:** 5-ALA, MAL, porcine skin, human skin, widefield fluorescence imaging, fluorescence spectroscopy, photodynamic therapy, skin models

© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2018 The Author(s). Licensee IntechOpen. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
