*2.5.2.3. Transesterification*

Transesterification is a process of acyl exchange between two molecules. This process normally takes place between an ester and alcohol (alcoholysis), an ester and an acid (acidolysis), or an ester with another ester (interesterification), and no water is involved in the reaction. Acidolysis is one of the most frequently used reactions to incorporate novel fatty acids into TAG in several researches [13, 73, 74]. Interesterification involving hydrolysis and esterification, firstly hydrolysis of the TAG molecule, then followed by re-synthesis of the liberated fatty acids onto the glycerol molecule. Interesterification is another main strategy to incorporate PUFAs into TAGs. The literature reported extensive research work on the interesterification reaction [75, 76]. Lipase-catalyzed alcoholysis, acidolysis, and interesterification reactions are described clearly in **Figure 6**.
