**2.9. Oxygen radical absorbance capacity (ORAC) assay**

For the ORAC assay, 5 g of fresh frozen fruit from different production systems was completely pestled in 15 mL of 50% acetone using a Polytron blender (Brinkmann Instruments, Westbury, NY, USA). The mixture was centrifuged at 10,000 rpm for 10 min, and the supernatant was kept in a 1.5-mL vial at −20°C.

The ORAC assay measures the capacity of the antioxidant to prevent the oxidization of a free radical (peroxyl) in biological systems. The assay was performed as described previously [29] with a Synergy™ Multi-Mode Microplate Reader (Bio-Tek Instruments, Inc., Winooski, VT, USA).
