**5. Conclusions**

This chapter discusses the importance of polyphenols as well as the availability of different methods to extract them from its natural sources. The most widely used methods in the extraction of polyphenols are the classical ones which usually includes maceration, percolation and successive Soxhlet extractions. Although these methods are still in use, they involve long extraction times, huge quantities of solvent, higher accumulation of residues and very limited results. Therefore, new methods such as UAE, MA, PLE, S-CO<sup>2</sup> and EAE have been developed in the recent years with very feasible results. Also, the evolution of separation and identification techniques of polyphenols has evolved from a simple colorimetry method to the most advanced chromatography techniques. However, the growing demand for new bioactive molecules from natural sources enhances the continuous search for new and innovative methods to extract and separate new molecules, which never ends.
