**3. Studies concerning biocompatibility of acrylic resins**

Acrylates are well known for their potential allergies. To evaluate the allergy potential of acrylic resins, we used *in vivo* and *in vitro* tests. For *in vivo* testing, we have used the patch test and the tumour necrosis alpha-factor (TNF) test, which is eloquent for cytotoxic detection and can detect early signs of allergy and inflammation by measuring endotoxins in patient serum. Acrylate toxicity is mainly due to the residual monomer. Therefore, we have also carried out tests to determine the amount of residual monomer present in the resin by the volatilecomponent-content method and bromine index methods [11, 12].
