**Meet the editors**

Fabio A. Marchi is a Scientific Researcher at A.C.Camargo Cancer Center in Sao Paulo, Brazil. He holds a PhD in Bioinformatics at Institute of Mathematics and Statistics, University of São Paulo, Brazil, and completed a postdoc in oncology investigating multidimensional data analysis. Dr. Marchi is actively involved in cancer research projects associated with big data, especially in systems biology area.

Priscila D. R. Cirillo is a biologist and S enior Researcher at Hermes Pardini Institute, R&D Division, Belo Horizonte, Brazil. She started her career at the Institute of Biosciences of Botucatu, São Paulo State University, Brazil, where she concluded her master's and PhD degrees in the field of cancer genetics. As postdoc at the Center for Translational Research in Oncology, São Paulo State Cancer Institute,

Brazil, she has involved mainly in fundamental aspects of tumor biology, focusing on mechanisms of chemoresistance. She published many articles about cancer genomics and also is a reviewer of Oncotarget journal (USA).

Elvis C. Mateo is a Scientific Coordinator of Research and Development Division (R&D) at Hermes Pardini Institute in Belo Horizonte, Brazil. He concluded his master's and PhD degrees in Human Genetics and Medical Science, all at the University of São Paulo, São Paulo, Brazil. Dr. Mateo is involved in the development of new diagnostic strategies using various molecular techniques.

Contents

**Preface VII**

Guedes

**Microorganisms 37**

**Section 1 Getting Started with RNA-Seq Data Analysis 1**

Chapter 2 **Practical Data Processing Approach for RNA Sequencing of**

Chapter 3 **Transcriptome Analysis for Non-Model Organism: Current**

Chapter 4 **Models of RNA Interaction from Experimental Datasets:**

Chapter 5 **Transcriptome Analysis of Non‐Coding RNAs in Livestock Species: Elucidating the Ambiguity 103**

Chapter 6 **Transcriptome Sequencing for Precise and Accurate**

**Cancer Datasets and Analysis 145**

Toshitaka Kumagai and Masayuki Machida

Michele Araújo Pereira, Eddie Luidy Imada and Rafael Lucas Muniz

Vahap Eldem, Gokmen Zararsiz, Tunahan Taşçi, Izzet Parug Duru,

Duy N. Do, Pier-Luc Dudemaine, Bridget Fomenky and Eveline M.

**Measurement of Transcripts and Accessibility of TCGA for**

Bijesh George, Vivekanand Ashokachandran, Aswathy Mary Paul

Chapter 1 **RNA‐seq: Applications and Best Practices 3**

**Status and Best-Practices 55**

Yakup Bakir and Melike Erkan

**Framework of Resilience 79**

William Seffens

Ibeagha-Awemu

and Reshmi Girijadevi
