**5. Perspectives and conclusions**

The bioinformatical description of GSC signalling dynamics based on the global quantitative phosphoproteome data led to network‐wide extraction of critical molecules and their related pathways for defining stemness characteristics. Further integrative description of multiple PTM dynamics in GSCs will deepen our understanding of the nature of their cell signalling complexity at the network level. We believe that shotgun proteomics‐based quantitative analyses of cancer stem cell signalling networks in combination with various statistical and mathematical platforms will pave the way to establish new directions towards systematic evaluation of drug targets in a cell‐type specific manner.
