**Application of Transmission Electron Microscopy Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals Techniques in the Veterinary Diagnosis of Viral Gastroenteritis in Livestock Animals**

**Application of Transmission Electron Microscopy** 

DOI: 10.5772/intechopen.70945

Marcia Helena Braga Catroxo and Ana Maria Cristina Rebello Pinto da Fonseca Martins Ana Maria Cristina Rebello Pinto da Fonseca Martins Additional information is available at the end of the chapter

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/intechopen.70945

Marcia Helena Braga Catroxo and

#### **Abstract**

[35] Erden A, Idilman R, Erden I, et al. Veins around the esophagus and the stomach: Do their calibrations provide a diagnostic clue for portal hypertensive gastropathy? Clinical

[36] Raul Cubillas, Don Rockey C. Portal hypertensive gastropathy: A review article. Liver

[37] Lo GH, Lai KH, Cheng JS, Hsu PI, Chen TA, Wang EM, Lin CK, Chiang HT. The effects of endoscopic variceal ligation and propranolol on portal hypertensive gastropathy: A

[38] Thuluvath PJ, Yoo HY. Portal hypertensive gastropathy. The American Journal of

[39] Wagatsuma Y, Naritaka Y, Shimakawa T. et al. Clinical usefulness of the angioten‐ sin II receptor antagonist losartan in patients with portal hypertensive gastropathy.

[40] Zhou Y, Qiao L, Wu J. et al. Comparison of the efficacy of octreotide, vasopressin, and omeprazole in the control of acute bleeding in patients with portal hypertensive gastrop‐ athy: A controlled study. Journal of Gastroenterology and Hepatology. 2002;**17**:973‐979

[41] Kamath PS, Lacerda M, Ahlquist DA, McKusick MA, Andrews JC, Nagorney DA. Gastric mucosal responses to intrahepatic portosystemic shunting in patients with cir‐

[42] De Franchis R, Faculty BV. Revising consensus in portal hypertension: report of the Baveno V consensus workshop on methodology of diagnosis and therapy in portal

[43] Smith LA, Morris AJ, Stanley AJ. The use of hemospray in portal hypertensive bleeding; A case series. European association for the study of the liver. Journal of Hepatology.

International . 2010;**30**(8):1094‐1102. DOI: 10.1111/j.1478‐3231.2010.02286

prospective, controlled trial. Gastrointestinal Endoscopy. 2001;**53**:579‐584

Imaging. 2009;**33**:22‐24

76 Stomach Disorders

Gastroenterology. 2002;**97**:2973‐2978

Hepatogastroenterology. 2006;**53**(68):171‐174

rhosis. Gastroenterology. 2000;**118**:905‐911

2014;**60**:457‐460

hypertension. Journal of Hepatology. 2010;**53**:762‐768

Gastroenteritis caused by viruses is considered to be one of the most important diseases in livestock, being the main cause of morbidity and mortality in young animals, culminating in serious economic losses due to costs with prophylaxis and treatment, increased susceptibility of animals to secondary infections, developmental delay and death. Stressful factors may support the onset of illness. Several viral agents can cause gastroenteritis in various animal species. Rotaviruses are considered the main cause of enteric infections in various animals, including humans constituting important zoonosis. Due to genetic diversity and their ability to cross the species barrier, the coronaviruses infect many species. In cattle, they cause "Winter Dysentery" in adult animals and "Neonatal Diarrhea" in newborn calves. In swine, they are responsible for "Transmissible Gastroenteritis" and "Swine Epidemic Diarrhea." Equines infected with coronavirus also develop severe gastroenteritis. Bovine viral diarrhea (BVD) caused by a flavivirus of the genus *Pestivirus* is related to digestive and reproductive disorders, affecting any productive sector, are it cut, milk or confinement. Transmission electron microscopy is an indispensable tool in the diagnosis of viral gastroenteric infectious diseases. Negative staining is a simple, fast and efficient technique, being ideal for the detection of gastroenteric viruses, being easily visualized. The immunoelectron microscopy (IEM) technique allows increasing the sensitivity of virus detection where low concentrations of virus are aggregated so that they may be more easily seen. The immunolabeling with colloidal gold technique utilizes specific antibodies tagged with particles of colloidal gold to label the antigen antibody reaction. Embedding resin technique allows obtaining information on the virus–cell interaction. The different transmission electron microscopy modalities promotes a fast and accurate diagnosis of the different gastroenteric viral agents, allowing prophylactic measures of control and prevention in the creations to be promptly instituted, avoiding animal losses and disastrous economic losses, and collaborating with the National Porcine and Bovine Agribusiness.

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© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons

**Keywords:** viral gastroenteritis, livestock animals, veterinary diagnostic, transmission electron microscopy
