5. Conclusion

4.1.2. The calculation of minimum inhibitory concentration (MIC)

Table 6. Antimicrobial activities of SHAM and its complexes.

Antibacterial bioassay Antifungal bioassay

Compd. S.a. S.g. B.s. P.a. E.c. E.c. A.f. C.a. A.a. P.i. S.c. M.c. SHAM þþþþ þþþþ þþþþ þþþþ þþþ þþþþ þþþþ þþþ þþþþ þþþþ þþþþ þþþþ I þþþ þþþ þþþ þþþ þþ þþ þþþþ þþþ þþþ þþþ þþþþ þþþ II þþ þþ þþ þþ þ þ þþ þþþ þþ þþ þ þ III þþþ þþþ þþþ þþþ þþ þþ þþþ þþ þþþ þþþ þþ þþþ IV þþþþ þþþþ þþþþ þþþþ þþþ þþþþ þþþþ þþþþ þþþ þþ þþþþ þþþ V þþþ þþþ þþþ þþþ þþ þþþ þþþþ þþ þþþ þþþþ þþ þþ VI þþþþ þþþ þþþþ þþþþ þþþþ þþþ þþþþ þþþ þþþ þþþ þþ þþþ A þþþ þþþ þþ þþ þþþ þþþþ þþþ þþ þþþþ þþ þþ þþ I, II, III, IV, V, VI are complexes of SHAM (I-VI) Cu(SHAM)2�2H2O (I), Ni(SHAM)2�2H2O (II), Fe(SHAM)2�2H2O (III), [Cu(Phen)(SHAM)] (IV), [Ni(Phen)(SHAM)] (V), [Fe(Phen)(SHAM)] (VI), and the reference drug (A) against six bacterial species and six fungi species in the agar disc diffusion method measured by diameter of inhibition zones

Bacterial strain Fungi strain

42 Descriptive Inorganic Chemistry Researches of Metal Compounds

after 1 day.

(DIZ, mm).

The apparatus used for the experiments are 0.5 mL volume 96-well microplates (ICN Biochem. Co., USA). Fill the first row of wells in each of 96-well microdilution plates with 100 μL of double strength TSB and fill the others with single strength TSB. Dilute the test compound and pour 100 μL in the first row. Every well filled must have its content mixed. The mixing technique based on suctioning and dispensing its content back five times. Take 100 μL from the first row and fill the second one. Then take from the second and fill the third. Repeat this procedure until the 23 rows. Each organism was experimented twice and the experiment as a whole was repeated three times. Before inoculating the 96 well plates with 96-prong inoculator, this later was in the inoculums. The microdilution plates were sealed with tape to prevent the drying of samples. At the end, plates were incubated at suitable temperatures to allow the growth to start. The growth was observed

Metal complexes of salicylhydroxamic acid (SHAM) and 1,10-phenanthroline (PHEN)

In this chapter, a detailed survey of the formation equilibria of Fe3<sup>þ</sup> with ligands of biological significance is presented. The main conclusions may be summarized as follows:

