**Biocatalysis of Rutin Hexadecanedioate Derivatives: Effect of Operating Conditions on Acylation Performance and Selectivity**

Manel Slimane, Aude Cordin, Mohamed Ghoul and Chebil Latifa

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/67621

#### **Abstract**

Rutin was enzymatically acylated with hexadecanedioic acid, in *tert*‐amyl alcohol, by an immobilized lipase from *Candida antartica* "Novozym 435". The effect of different tech‐ niques of water removal, temperature, concentration of rutin and diacid/rutin molar ratio was investigated. The obtained results indicated that drying the media by adding the molecular sieves in the outer loop of the reactor was the most efficient method leading to water content lower than 200 ppm. The highest performances (conversion yield and initial rate) were reached at 90°C, 131 mM of rutin, and 118 mM of acid. Depending on the water content and the diacid/rutin molar ratio, only rutin 4‴‐hexadecanedioate or both rutin 4‴‐hexadecanedioate and dirutin 4‴, 4‴‐hexadecanedioate were synthesized.

**Keywords:** biocatalysis, lipase, rutin, hexadecanedioic acid, water activity
