**Procedures for Detection of Resistant Weeds Using 14C-Herbicide Absorption, Translocation, and Metabolism Using <sup>14</sup>C-Herbicide Absorption, Translocation, and Metabolism**

**Procedures for Detection of Resistant Weeds** 

DOI: 10.5772/68092

Kassio Ferreira Mendes, Ricardo Ferraz Silveira, Silveira, Miriam Hiroko Inoue and

Kassio Ferreira Mendes, Ricardo Ferraz

Miriam Hiroko Inoue and Valdemar Luiz Tornisielo Valdemar Luiz Tornisielo

Additional information is available at the end of the chapter Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/68092

#### **Abstract**

Herbicide resistance mechanisms involve altered absorption, translocation, and metabolism of herbicides (i.e., glyphosate), and this is an important component in the study of herbicide resistance mechanisms as well. 14C-herbicides are used in resistant weeds studies, since they provide some advantages in comparison with chemical measures, including greater sensitivity, stepwise description of a particular element in a metabolic system, herbicide position, detection through X-ray films and/or radio image, and liquid scintillation. However, an up-to-date, organized description and standardization of research procedures and methodology on the use of radioisotopes for detection of resistant weeds, through different mechanisms of absorption, translocation, and metabolism in comparison with susceptible weeds are lacking in the literature. Techniques that use 14C such as tracers are extremely useful to study the herbicides behavior in the resistant weed, since the radiometric techniques offer the possibility of accurately determining very small amounts in a relatively short time. However, mechanism of resistance to herbicides in this resistant weed population compared with the susceptible population cannot be due to differential absorption, translocation, or metabolism of herbicide in weed; so other studies are necessary to elucidate the mechanism of herbicide resistance on weed population.

**Keywords:** mechanism of resistance, metabolites, standard methodology, radioisotopes

© 2016 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2017 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
