**5.1.1.2 Hexane/ether (1:1, v/v) method**

A known weight of each ground dried algal species (10 g dry weight) was mixed with the extraction solvent mixture, hexane/ether (100 ml, 1:1, v/v), kept to settle for 24 hrs, followed by filtration (Fig. 1) according to Hossain and Salleh (2008).
