**5.1.1.1 Chloroform /methanol (2:1, v/v) method**

A known weight of each ground dried algal species (10 g dry weight) was mixed separately with the extraction solvent mixture; chloroform/methanol (100 ml, 2:1, v/v) for 20 min. using shaker, followed by the addition of mixture of chloroform/water (50 ml, 1:1, v/v) for 10 min. filter and the algal residue was extracted three times by 100 ml chloroform followed by filtration (Fig.1) according to Bligh and Dayer (1959).
