**High Affinity Maturated Human Antibodies from Naïve and Synthetic Antibody Repertoires and Synthetic Antibody Repertoires**

**High Affinity Maturated Human Antibodies from Naïve** 

DOI: 10.5772/intechopen.71664

Chia Chiu Lim, Yee Siew Choong and Theam Soon Lim Additional information is available at the end of the chapter

Additional information is available at the end of the chapter

Chia Chiu Lim, Yee Siew Choong and Theam Soon Lim

http://dx.doi.org/10.5772/intechopen.71664

#### **Abstract**

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DOI: 10.1038/ni920

16 Antibody Engineering

090740

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Recombinant human antibody technology has been the cornerstone of the uprising of biologics in the pharmaceutical industry. The introduction of various display technologies like phage, yeast, bacterial, ribosomal, mRNA, DNA display and mammalian cell surface display has allowed improved antibody generation programs. The ability to generate recombinant antibodies from available human antibody libraries by using *in vitro* display methods pave the way to select recombinant human antibodies against almost every antigen. The libraries are a close representation of the B-cell response elicited by the natural immune system. The introduction of various methods to fine tune the antibody affinities has made recombinant antibody technology highly sought after. The ability to engineer specific characteristics of each antibody by design is possible utilizing advanced *in vitro* strategies. This chapter will focus on the technologies commonly applied in antibody display technologies to engineer improved affinities.

**Keywords:** naïve- immune- and synthetic- antibody library, *in vitro* display systems, affinity maturation, *de novo* antibody gene synthesis, genome editing techniques
