**Meet the editor**

Thomas Böldicke received his PhD 1982 at the Max-Planck-Institut of Molecular Genetics, Berlin. He started his carrier as post doc at the German Research Centre for Biotechnology (GBF, Brunswick) in the Department of Genetics and Cell Biology by John Collins. Now he is senior scientist at the Helmholtz Centre for Infection Research (HZI, former GBF) and project leader

intrabodies. 2011 he qualified as a professor in molecular biology and cell biology at the Technical University of Braunschweig, Germany. He is an expert in generating mouse and human hybridomas and in selecting and modifying recombinant antibodies. In the last decade he focused on the construction and characterization of intracellular antibodies. He has published 35 manuscripts.

Contents

**Preface VII**

Oliver Backhaus

**Libraries 75**

**Secretion 119**

Atis Chakrabarti

Naito

Chapter 1 **Generation of Antibody Diversity 1**

Chapter 2 **High Affinity Maturated Human Antibodies from Naïve and**

Chia Chiu Lim, Yee Siew Choong and Theam Soon Lim

Lilian Rumi Tsuruta, Mariana Lopes dos and Ana Maria Moro

**Antibodies from Combinatorial Immune Phage Display**

**Synthetic Antibody Repertoires 17**

**Antibodies for Clinical Use 47**

Chapter 3 **Display Technologies for the Selection of Monoclonal**

Chapter 4 **Detailed Protocols for the Selection of Antiviral Human**

Chapter 5 **Colony Assay for Antibody Library Screening: Outlook and**

Chapter 6 **Construction and Characteristics of a Recombinant Single-Chain Antibody Fragment against Bacterial Type III**

Chapter 7 **Separation of Monoclonal Antibodies by Analytical Size**

**Exclusion Chromatography 133**

Teiji Sawa, Atsushi Kainuma, Kiyoshi Moriyama and Yoshifumi

Philipp Diebolder and Adalbert Krawczyk

**Comparison to Display Screening 103**

Mieko Kato and Yoshiro Hanyu
