**3. Equipment of the radio-isotope laboratory (IOCB)**

The author of this chapter is a member of the Radio-isotope laboratory, a service group of the IOCB CAS, working as a synthetic radiochemist in the production of radioactive molecular tracers. The laboratory is classified for handling of the open sources of ionizing radiation in quantities authorized for laboratories of II category according to Czech bylaw 307/2002 Sb for research and development and educational purposes. It is currently authorized to work with the main radioactive isotopes used in research, for example, 3 H, 14C, 32P, 33P, 35S, 51Cr, 54Mn, 55Fe, 99Tc and <sup>125</sup>I. The main purpose of the laboratory is to provide a series of highly specific facilities, equipment and services fully adapted to researchers' needs and maintained in optimum conditions, always in line with applicable legislation to ensure that all personnel is fully protected and ensuring the physical safety of the materials used and the environment. The laboratory has an equipment, stuff, and knowledge to cooperate with other chemists and biologists to provide them with custom synthesis of radio-labeled compounds, especially commercially unavailable compounds. Following time-depending stability of synthesized radio-labeled compounds is one of our basic services provided to biologists.

The key instrument of the laboratory is a glove box with tritiation manifold from RC-TRITEC AG (Teufen, Switzerland) suitable for handling 100–1000 Ci of carrier-free tritium gas (**Figure 15**). Tritiation manifold is based on U-Bed Technology to provide fresh,

**3. Equipment of the radio-isotope laboratory (IOCB)**

26 Phytohormones - Signaling Mechanisms and Crosstalk in Plant Development and Stress Responses

**Figure 14.** Synthetic pathway to 24-[14C]epiCS and 24-[14C]epiBL.

work with the main radioactive isotopes used in research, for example, 3

biologists.

The author of this chapter is a member of the Radio-isotope laboratory, a service group of the IOCB CAS, working as a synthetic radiochemist in the production of radioactive molecular tracers. The laboratory is classified for handling of the open sources of ionizing radiation in quantities authorized for laboratories of II category according to Czech bylaw 307/2002 Sb for research and development and educational purposes. It is currently authorized to

51Cr, 54Mn, 55Fe, 99Tc and <sup>125</sup>I. The main purpose of the laboratory is to provide a series of highly specific facilities, equipment and services fully adapted to researchers' needs and maintained in optimum conditions, always in line with applicable legislation to ensure that all personnel is fully protected and ensuring the physical safety of the materials used and the environment. The laboratory has an equipment, stuff, and knowledge to cooperate with other chemists and biologists to provide them with custom synthesis of radio-labeled compounds, especially commercially unavailable compounds. Following time-depending stability of synthesized radio-labeled compounds is one of our basic services provided to

H, 14C, 32P, 33P, 35S,

**Figure 15.** The equipment for safe handling of tritium gas; the tritium manifold (RC-TRITEC AG) placed in the glove box; a scrubber for decontamination placed behind the glove box.

3 He-free tritium for tritiation by simply heating the UT3 -bed, also allowing the recovery of surplus gas after completion of a reaction (**Figure 16**). During the operation of the manifold, the internal atmosphere of the glove box is continually decontaminated by a scrubber equipped with catalytic oxidation of gaseous tritium to tritiated water, which is trapped

**Figure 16.** The equipment for safe handling of tritium gas; the tritium manifold (RC-TRITEC AG) placed in the glove box.

on a molecular sieve. 3 H NMR measurements were performed on a Bruker Avance II 300 MHz in the laboratory. Liquid scintillation analyser Tri-Carb 2900TR (Perkin Elmer) was used for detecting small amounts of α, β, and γ radioactivity. Mobile contamination monitor CoMo 170 (GRAETZ Strahlungsmeßtechnik GmbH) was used for the high-sensitive and nuclide referred measurement of surfaces with regard to α-, β-, and γ-contaminations when handling ionizing radiation. Analytical -preparative radio-HPLC (pump Waters 600, UV detector Waters 2487, radio chromatogram detector Ramona with analytical cell (LSC) and solid scintillator preparative cell (Raytest, Germany), data management software Empower 2 from Waters). Basic radiation protection equipment and waste disposal management.
