5.1. Thermostats and temperature control

By its morphological features, L41 cell line is an even monolayer of distinct epithelium-like polygonal or roundish cells; there is a constant 4–6% share of giant cells. The cytoplasm is finegranular. The nuclei are roundish and contain 2–4 nucleoli. There are up to 6% abnormal mitosis forms. The share of cells with irregularly shaped nuclei is 8%. The monolayer was generated on day 3–5 from planting into a medium consisting of equal parts of Eagle medium and 199 medium with 10% bovine embryo serum. The cell maintenance medium contains

The culture is highly sensitive to poliomyelitis, Coxsackie B, ECHO-19, human adenovirus,

Vero cell line was derived from normal simian renal cells (those of an adult African green monkey). The number of generations and passages: over 120 passages before the test start. The line has been used in a laboratory research since 1962. The monolayer forms on days 3–5 from

The morphological features: epithelium-like cells, polygonal, with notable vacuolization, and

The karyological characteristics: the cells correspond to the monkey karyotype by their struc-

Species origin: monkey, confirmed karyologically. Data on contamination: no bacteria, fungi, or Mycoplasma detected. The cell line is maintained in the growth medium + 10% glycerin in liquid nitrogen. About 80–85% cells restore on defrosting. The culture is highly sensitive to

HLE-3 cell line was derived by Yekaterinburg Institute of Viral Infections (YRIVI) staff from normal human lung tissue. The technique for obtaining this cell line was developed in the cell line laboratory of YRIVI by Glinskikh et al. in 1980 from the lung of a 12-week human embryo from a healthy female whose genelogy was free from malignant or hereditary diseases.

The monolayer forms on days 4 and 5 from the planting moment. The morphological features:

The karyological characteristics: the cells correspond to the human karyotype by their struc-

Data on contamination: no bacteria, fungi, or Mycoplasma detected. The cell line is maintained in the growth medium +10% glycerin in liquid nitrogen. About 70% of the cells restore on

Sensitivity to viruses: the culture is highly sensitive to polioviruses 1, 2, and 3, Coxsackie B3,

ture. The modal class contains 87% cells with normal diploid human chromosome set.

Species origin: human, confirmed karyologically with an isoenzyme technique.

BArinsECHO 3, 6, 11, 13, 19, 20, 24, 28, RS viruses, and herpes simplex virus.

The number of generations and passages: 20 passages at most before the test start.

necessary amino acids, salts, and glucose.

distinctly oriented growth zones.

poliomyelitis viruses and arboviruses.

fibroblastic cells with distinct edges.

defrosting.

the planting moment. The multiplying factor is 6–7 on day 5.

ture—44 diploid cells, 3 hyperploid cells, and 53% hypoploid cells.

and measles viruses.

118 Optical Interferometry

Precise maintenance of the temperature in the medium for the cells plays an important role in the experiments. After several attempts to create a small thermal chamber to maintain the temperature in the small region, we decided on placing the entire optical system into a thermostat of suitable dimensions. We used three thermostats: (a) a self-made laboratory thermostat, (b) a liquid thermostat of ЗЦ-1125М type, and (c) an air bath of ТСЭ-200 type.

We used the laboratory thermostat in the cases when we had to place the substrate with cells in a horizontal position. The photo of the thermostat is shown in Figure 3.

Figure 3. Photograph of laboratory thermostat.

We used a dustproof chamber of a scanning atomic-force microscope. To heat the air inside the chamber, we used a liquid ultrathermostat U10 connected to the radiator with pipes which is common for Russia. The radiator was in the lower part of the chamber under the table for the microscope: it was blown over by six small ventilators. A metallographic microscope of Axio 40 MAT type that we used to generate speckle images of the cells was placed on the table.

The temperature of the cuvette in all the thermostats was determined by a temperature sensor of DS18В20 type of precision to ±0.1°С. The signals from the sensor entered the computer; the temperature values were displayed in the monitor or recorded into the computer memory in the preset time.

The laboratory thermostat maintained the temperature of the cuvette to ±0.1°С precision for several hours, and the liquid thermostat ЗЦ-1125М did so for several days. An air bath was used to study the cell reaction to temperature.
