**5.3 Integration site of BAC transgene**

Chandler and coworkers were also able to identify multiple integration sites in BAC reporters. This was achieved by employing the BAC transgene copy number assay described above and analyzing outcrossed F2 generations for the number of transgene copies (Chandler, Chandler et al. 2007). Since the level of reporter expression is dependent on transgene copy number, it is useful to identify founders that transmit a single integration site, ensuring consistent reporter expression in future progeny.
