**2.6 Solutions used in the present study**

*LB*: One litre of LB solution contains 10 g of Tryptone (BD Biosciences), 5 g of Yeast Extract (BD Biosciences), 5 g of NaCl and 4 ml of 1N NaOH. The solution is stored at room temperature after autoclave.

*Solution I for BAC purification*: This solution contains 50 mM glucose, 25 mM Tris-Cl (pH 8.0) and 10 mM EDTA (pH 8.0). The solution was stored at room temperature.

*Solution II for BAC purification*: This solution contains 1% sodium dodecyl sulphate (SDS) and 0.2N NaOH. To obtain a good amount and the purity of the BACs, the solution was freshly prepared from 10% SDS and 2N NaOH stock solutions stored at room temperature.

*Solution III for BAC purification*: 3M potassium acetate was dissolved into distilled water and the pH is adjusted with formic acid to 4.8. We store the solution at -20C.

*Phenol Chloroform mixture*: 10mM Tris-Cl saturated phenol solution was mixed with the 0.96 volume of chloroform and 0.04 volume of isoamyl alcohol. The mixture was stored at 4C.

*PBS*: One litre of 10x PBS stock contains 80 g of NaCl, 2 g of KCl, 11.5 g of Na2HPO4 and 2 g of KH2PO4. We sterilised the stock solution by autoclaving and stored it at room temperature.

*TAE*: One litre of 50x TAE solution contains 242 g of Tris-Cl, 57.1 ml of CH3COOH and 100 ml of 0.5M EDTA (pH8.0).

*TBE*: One litre of 10x TBE solution contains 108 g of Tris-Cl, 55 g of boric acid and 40 ml of 0.5M EDTA (pH8.0).

*Tyrode's solution*: Five litres of Tyrode's solution contains 40.0 g of NaCl, 1.0 g of KCl, 1.0 g of CaCl2, 1.05 g of MgCl2·6H2O, 0.285 g of NaH2PO4·2H2O, 5.0 g of NaHCO3 and 5.0 g of glucose. To avoid the possible precipitation of salts, we added these reagents in this order and sterilised by filtration and stored at 4C.
