**3. Application of VZVBAC with a luciferase marker**

Visualization markers are often inserted into the viral BAC genome to detect and quantify viral replication. Two methods frequently utilized are fluorescence imaging and bioluminescence imaging. Fluorescence-based imaging, such as through the use of a GFP reporter gene, is a common method to monitor *in vitro* infections and allows researchers to study the interaction of a given virus with its host (Tang, 2008). Visualization of *in vivo* infections on the other hand, can be established by the use of bioluminescence imaging (BLI).
