**4. Conclusions and overall perspectives**

Studies on BACs have demonstrated their importance in many research fields, from microbiology, virology, to human genetics, neuroscience, and proteomics (Narayanan 2008; Adamson, Jackson et al. 2011). The power to clone and handle large sized intact genome with high fidelity by BAC has enabled scientists to design and perform both mechanistic and functional studies in an ever expanding field.
