**2.4. The determination of cardiac and ventilation rates**

The heart and respiratory rates were measured by determining the number of heart frequencies, as well as by observing the respiratory thoracic movements, using the stethoscope for 1 min. These estimations were detected out by the same person prior to blood test for analysis, according to earlier article [20].

The lack of effect of solvents was determined in preliminary experiments [29, 32].
