**2.4. Determination of pH and free acidity of honey**

A honey sample of 10 g was accurately weighed in a 250 ml beaker on an electronic balance. The honey sample was then dissolved in 75 ml of carbon dioxide-free water (freshly produced de-ionised water) in a 250 ml beaker. The pH was recorded (Orion Star A215 Thermo Scientific). For the measurement of free acidity, the above solution was titrated with 0.1 M NaOH solution to pH 8.30 using the automatic titrator (Kern. Model: ABT-120-5DM).
