**5. Conclusion**

In conclusion, Nile tilapia sperm can be successfully cryopreserved in a glucose-Tris–based cryosolution containing 10% DMA with 0.5-mL straws. The applied protocol can be used in commercial hatcheries to facilitate artificial reproduction of Nile tilapia due to acceptable postthaw motility and fertility results obtained. On the other hand, additional researches are needed to examine the growth and survival of the larvae originated from cryopreserved sperm.
